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Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3

Choriocarcinoma cells BeWo b30 are used to model human placental trophoblast hypoxia using cobalt (II) chloride and hydroxyquinoline derivative (HD) as chemical inducers of hypoxia-inducible factor (HIF). In this study, it was shown that both substances activate the hypoxic pathway and the epithelia...

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Autores principales: Knyazev, E. N., Paul, S. Yu., Tonevitsky, A. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382627/
https://www.ncbi.nlm.nih.gov/pubmed/34426922
http://dx.doi.org/10.1134/S1607672921040104
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author Knyazev, E. N.
Paul, S. Yu.
Tonevitsky, A. G.
author_facet Knyazev, E. N.
Paul, S. Yu.
Tonevitsky, A. G.
author_sort Knyazev, E. N.
collection PubMed
description Choriocarcinoma cells BeWo b30 are used to model human placental trophoblast hypoxia using cobalt (II) chloride and hydroxyquinoline derivative (HD) as chemical inducers of hypoxia-inducible factor (HIF). In this study, it was shown that both substances activate the hypoxic pathway and the epithelial–mesenchymal transition and inhibit the pathways of cell proliferation. However, CoCl(2) caused activation of the apoptosis pathway, increased the activity of effector caspases 3 and 7, and increased the expression of the unfolded protein response target DDIT3. The mTORC1 pathway was activated upon exposition to CoCl(2), while HD suppressed this pathway, as it happens during real trophoblast hypoxia. Thus, effect of CoCl(2) on BeWo cells can be a model of severe hypoxia with activation of apoptosis, while HD mimics moderate hypoxia.
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spelling pubmed-83826272021-09-09 Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3 Knyazev, E. N. Paul, S. Yu. Tonevitsky, A. G. Dokl Biochem Biophys Biochemistry, Biophysics, and Molecular Biology Choriocarcinoma cells BeWo b30 are used to model human placental trophoblast hypoxia using cobalt (II) chloride and hydroxyquinoline derivative (HD) as chemical inducers of hypoxia-inducible factor (HIF). In this study, it was shown that both substances activate the hypoxic pathway and the epithelial–mesenchymal transition and inhibit the pathways of cell proliferation. However, CoCl(2) caused activation of the apoptosis pathway, increased the activity of effector caspases 3 and 7, and increased the expression of the unfolded protein response target DDIT3. The mTORC1 pathway was activated upon exposition to CoCl(2), while HD suppressed this pathway, as it happens during real trophoblast hypoxia. Thus, effect of CoCl(2) on BeWo cells can be a model of severe hypoxia with activation of apoptosis, while HD mimics moderate hypoxia. Pleiades Publishing 2021-08-23 2021 /pmc/articles/PMC8382627/ /pubmed/34426922 http://dx.doi.org/10.1134/S1607672921040104 Text en © The Author(s) 2021, ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2021, Vol. 499, pp. 251–256. © The Author(s), 2021. This article is an open access publication.Russian Text © The Author(s), 2021, published in Doklady Rossiiskoi Akademii Nauk. Nauki o Zhizni, 2021, Vol. 499, pp. 375–380. https://creativecommons.org/licenses/by/4.0/Open Access.This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biochemistry, Biophysics, and Molecular Biology
Knyazev, E. N.
Paul, S. Yu.
Tonevitsky, A. G.
Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3
title Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3
title_full Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3
title_fullStr Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3
title_full_unstemmed Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3
title_short Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3
title_sort chemical induction of trophoblast hypoxia by cobalt chloride leads to increased expression of ddit3
topic Biochemistry, Biophysics, and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382627/
https://www.ncbi.nlm.nih.gov/pubmed/34426922
http://dx.doi.org/10.1134/S1607672921040104
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