Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube

Cryopreservation of whole blood is useful for DNA collection, and clinical and basic research. Blood samples in ethylenediaminetetraacetic acid disodium salt (EDTA) tubes stored at − 80 °C are suitable for DNA extraction, but not for high-quality RNA extraction. Herein, a new methodology for high-qu...

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Detalles Bibliográficos
Autores principales: Yamagata, Hirotaka, Kobayashi, Ayumi, Tsunedomi, Ryouichi, Seki, Tomoe, Kobayashi, Masaaki, Hagiwara, Kosuke, Chen, Chong, Uchida, Shusaku, Okada, Go, Fuchikami, Manabu, Kamishikiryo, Toshiharu, Iga, Jun-ichi, Numata, Shusuke, Kinoshita, Makoto, Kato, Takahiro A., Hashimoto, Ryota, Nagano, Hiroaki, Okamoto, Yasumasa, Ueno, Shuichi, Ohmori, Tetsuro, Nakagawa, Shin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382694/
https://www.ncbi.nlm.nih.gov/pubmed/34426633
http://dx.doi.org/10.1038/s41598-021-96567-2

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