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Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants

Iodine deficiency represents a public health problem worldwide. To increase the amount of iodine in the diet, biofortification strategies of plants have been tried. They rely on the exogenous administration of iodine to increase its absorption and accumulation. However, iodine is not stable in plant...

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Autores principales: Carlessi, Martina, Mariotti, Lorenzo, Giaume, Francesca, Fornara, Fabio, Perata, Pierdomenico, Gonzali, Silvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382704/
https://www.ncbi.nlm.nih.gov/pubmed/34426588
http://dx.doi.org/10.1038/s41598-021-95198-x
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author Carlessi, Martina
Mariotti, Lorenzo
Giaume, Francesca
Fornara, Fabio
Perata, Pierdomenico
Gonzali, Silvia
author_facet Carlessi, Martina
Mariotti, Lorenzo
Giaume, Francesca
Fornara, Fabio
Perata, Pierdomenico
Gonzali, Silvia
author_sort Carlessi, Martina
collection PubMed
description Iodine deficiency represents a public health problem worldwide. To increase the amount of iodine in the diet, biofortification strategies of plants have been tried. They rely on the exogenous administration of iodine to increase its absorption and accumulation. However, iodine is not stable in plants and can be volatilized as methyl iodide through the action of specific methyltransferases encoded by the HARMLESS TO OZONE LAYER (HOL) genes. The release of methyl iodide in the atmosphere represents a threat for the environment due to its ozone depletion potential. Rice paddies are among the strongest producers of methyl iodide. Thus, the agronomic approach of iodine biofortification is not appropriate for this crop, leading to further increases of iodine emissions. In this work, we used the genome editing CRISPR/Cas9 technology to knockout the rice HOL genes and investigate their function. OsHOL1 resulted a major player in methyl iodide production, since its knockout abolished the process. Moreover, its overexpression reinforced it. Conversely, knockout of OsHOL2 did not produce effects. Our experiments helped elucidating the function of the rice HOL genes, providing tools to develop new rice varieties with reduced iodine emissions and thus more suitable for biofortification programs without further impacting on the environment.
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spelling pubmed-83827042021-09-01 Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants Carlessi, Martina Mariotti, Lorenzo Giaume, Francesca Fornara, Fabio Perata, Pierdomenico Gonzali, Silvia Sci Rep Article Iodine deficiency represents a public health problem worldwide. To increase the amount of iodine in the diet, biofortification strategies of plants have been tried. They rely on the exogenous administration of iodine to increase its absorption and accumulation. However, iodine is not stable in plants and can be volatilized as methyl iodide through the action of specific methyltransferases encoded by the HARMLESS TO OZONE LAYER (HOL) genes. The release of methyl iodide in the atmosphere represents a threat for the environment due to its ozone depletion potential. Rice paddies are among the strongest producers of methyl iodide. Thus, the agronomic approach of iodine biofortification is not appropriate for this crop, leading to further increases of iodine emissions. In this work, we used the genome editing CRISPR/Cas9 technology to knockout the rice HOL genes and investigate their function. OsHOL1 resulted a major player in methyl iodide production, since its knockout abolished the process. Moreover, its overexpression reinforced it. Conversely, knockout of OsHOL2 did not produce effects. Our experiments helped elucidating the function of the rice HOL genes, providing tools to develop new rice varieties with reduced iodine emissions and thus more suitable for biofortification programs without further impacting on the environment. Nature Publishing Group UK 2021-08-23 /pmc/articles/PMC8382704/ /pubmed/34426588 http://dx.doi.org/10.1038/s41598-021-95198-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Carlessi, Martina
Mariotti, Lorenzo
Giaume, Francesca
Fornara, Fabio
Perata, Pierdomenico
Gonzali, Silvia
Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants
title Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants
title_full Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants
title_fullStr Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants
title_full_unstemmed Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants
title_short Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants
title_sort targeted knockout of the gene oshol1 removes methyl iodide emissions from rice plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382704/
https://www.ncbi.nlm.nih.gov/pubmed/34426588
http://dx.doi.org/10.1038/s41598-021-95198-x
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