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Serum IgA Antibodies Specific to M. leprae Antigens as Biomarkers for Leprosy Detection and Household Contact Tracking

Leprosy remains endemic in several developing countries, such as India and Brazil, in part due to delayed diagnosis that facilitates ongoing transmission. Although immunoglobulins against several Mycobacterium leprae antigens have been indicated for the early diagnosis, and IgA participates in the e...

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Autores principales: Silva, Kyssia Karen de Paiva e, de Oliveira, Erick Esteves, Elias, Carolina Martins Moreira, Pereira, Ingrid Estevam, Pinheiro, Roberta Olmo, Sarno, Euzenir Nunes, Duthie, Malcolm Scott, Teixeira, Henrique Couto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382955/
https://www.ncbi.nlm.nih.gov/pubmed/34447763
http://dx.doi.org/10.3389/fmed.2021.698495
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author Silva, Kyssia Karen de Paiva e
de Oliveira, Erick Esteves
Elias, Carolina Martins Moreira
Pereira, Ingrid Estevam
Pinheiro, Roberta Olmo
Sarno, Euzenir Nunes
Duthie, Malcolm Scott
Teixeira, Henrique Couto
author_facet Silva, Kyssia Karen de Paiva e
de Oliveira, Erick Esteves
Elias, Carolina Martins Moreira
Pereira, Ingrid Estevam
Pinheiro, Roberta Olmo
Sarno, Euzenir Nunes
Duthie, Malcolm Scott
Teixeira, Henrique Couto
author_sort Silva, Kyssia Karen de Paiva e
collection PubMed
description Leprosy remains endemic in several developing countries, such as India and Brazil, in part due to delayed diagnosis that facilitates ongoing transmission. Although immunoglobulins against several Mycobacterium leprae antigens have been indicated for the early diagnosis, and IgA participates in the early stages of leprosy and in subclinical infection, relatively little research has examined anti-M. leprae IgA responses. Here, we investigated serum IgA reactivity against NDO-HSA, LID-1 and NDO-LID, in paucibacillary (PB) and multibacillary (MB) patients and their household contacts, using enzyme-linked immunosorbent assay (ELISA). Diagnostic accuracy of each ELISA was evaluated by receiver operating characteristic (ROC) curve analysis. Our data reveal elevated IgA serum levels against the three M. leprae specific antigens in MB patients, whereas IgA reactivity in PB patients was increased only to NDO-HSA. Further, MB and PB household contacts displayed higher IgA reactivity to NDO-HSA than non-endemic controls. Our data suggest measurement of serum IgA against NDO-HSA as an additional tool in the diagnosis and classification of the disease, with potential utility for household contact follow-up.
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spelling pubmed-83829552021-08-25 Serum IgA Antibodies Specific to M. leprae Antigens as Biomarkers for Leprosy Detection and Household Contact Tracking Silva, Kyssia Karen de Paiva e de Oliveira, Erick Esteves Elias, Carolina Martins Moreira Pereira, Ingrid Estevam Pinheiro, Roberta Olmo Sarno, Euzenir Nunes Duthie, Malcolm Scott Teixeira, Henrique Couto Front Med (Lausanne) Medicine Leprosy remains endemic in several developing countries, such as India and Brazil, in part due to delayed diagnosis that facilitates ongoing transmission. Although immunoglobulins against several Mycobacterium leprae antigens have been indicated for the early diagnosis, and IgA participates in the early stages of leprosy and in subclinical infection, relatively little research has examined anti-M. leprae IgA responses. Here, we investigated serum IgA reactivity against NDO-HSA, LID-1 and NDO-LID, in paucibacillary (PB) and multibacillary (MB) patients and their household contacts, using enzyme-linked immunosorbent assay (ELISA). Diagnostic accuracy of each ELISA was evaluated by receiver operating characteristic (ROC) curve analysis. Our data reveal elevated IgA serum levels against the three M. leprae specific antigens in MB patients, whereas IgA reactivity in PB patients was increased only to NDO-HSA. Further, MB and PB household contacts displayed higher IgA reactivity to NDO-HSA than non-endemic controls. Our data suggest measurement of serum IgA against NDO-HSA as an additional tool in the diagnosis and classification of the disease, with potential utility for household contact follow-up. Frontiers Media S.A. 2021-08-10 /pmc/articles/PMC8382955/ /pubmed/34447763 http://dx.doi.org/10.3389/fmed.2021.698495 Text en Copyright © 2021 Silva, Oliveira, Elias, Pereira, Pinheiro, Sarno, Duthie and Teixeira. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Silva, Kyssia Karen de Paiva e
de Oliveira, Erick Esteves
Elias, Carolina Martins Moreira
Pereira, Ingrid Estevam
Pinheiro, Roberta Olmo
Sarno, Euzenir Nunes
Duthie, Malcolm Scott
Teixeira, Henrique Couto
Serum IgA Antibodies Specific to M. leprae Antigens as Biomarkers for Leprosy Detection and Household Contact Tracking
title Serum IgA Antibodies Specific to M. leprae Antigens as Biomarkers for Leprosy Detection and Household Contact Tracking
title_full Serum IgA Antibodies Specific to M. leprae Antigens as Biomarkers for Leprosy Detection and Household Contact Tracking
title_fullStr Serum IgA Antibodies Specific to M. leprae Antigens as Biomarkers for Leprosy Detection and Household Contact Tracking
title_full_unstemmed Serum IgA Antibodies Specific to M. leprae Antigens as Biomarkers for Leprosy Detection and Household Contact Tracking
title_short Serum IgA Antibodies Specific to M. leprae Antigens as Biomarkers for Leprosy Detection and Household Contact Tracking
title_sort serum iga antibodies specific to m. leprae antigens as biomarkers for leprosy detection and household contact tracking
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382955/
https://www.ncbi.nlm.nih.gov/pubmed/34447763
http://dx.doi.org/10.3389/fmed.2021.698495
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