High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

Significant technical challenges have limited the study of extremophile cell biology. Here we describe a system for imaging samples at 75°C using high numerical aperture, oil-immersion lenses. With this system we observed and quantified the dynamics of cell division in the model thermoacidophilic cr...

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Autores principales: Charles-Orszag, Arthur, Lord, Samuel J., Mullins, R. Dyche
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383144/
https://www.ncbi.nlm.nih.gov/pubmed/34447359
http://dx.doi.org/10.3389/fmicb.2021.707124
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author Charles-Orszag, Arthur
Lord, Samuel J.
Mullins, R. Dyche
author_facet Charles-Orszag, Arthur
Lord, Samuel J.
Mullins, R. Dyche
author_sort Charles-Orszag, Arthur
collection PubMed
description Significant technical challenges have limited the study of extremophile cell biology. Here we describe a system for imaging samples at 75°C using high numerical aperture, oil-immersion lenses. With this system we observed and quantified the dynamics of cell division in the model thermoacidophilic crenarchaeon Sulfolobus acidocaldarius with unprecedented resolution. In addition, we observed previously undescribed dynamic cell shape changes, cell motility, and cell-cell interactions, shedding significant new light on the high-temperature lifestyle of this organism.
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spelling pubmed-83831442021-08-25 High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius Charles-Orszag, Arthur Lord, Samuel J. Mullins, R. Dyche Front Microbiol Microbiology Significant technical challenges have limited the study of extremophile cell biology. Here we describe a system for imaging samples at 75°C using high numerical aperture, oil-immersion lenses. With this system we observed and quantified the dynamics of cell division in the model thermoacidophilic crenarchaeon Sulfolobus acidocaldarius with unprecedented resolution. In addition, we observed previously undescribed dynamic cell shape changes, cell motility, and cell-cell interactions, shedding significant new light on the high-temperature lifestyle of this organism. Frontiers Media S.A. 2021-08-10 /pmc/articles/PMC8383144/ /pubmed/34447359 http://dx.doi.org/10.3389/fmicb.2021.707124 Text en Copyright © 2021 Charles-Orszag, Lord and Mullins. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Charles-Orszag, Arthur
Lord, Samuel J.
Mullins, R. Dyche
High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius
title High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius
title_full High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius
title_fullStr High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius
title_full_unstemmed High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius
title_short High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius
title_sort high-temperature live-cell imaging of cytokinesis, cell motility, and cell-cell interactions in the thermoacidophilic crenarchaeon sulfolobus acidocaldarius
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383144/
https://www.ncbi.nlm.nih.gov/pubmed/34447359
http://dx.doi.org/10.3389/fmicb.2021.707124
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