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Comparative phylogenetic analysis of CBL reveals the gene family evolution and functional divergence in Saccharum spontaneum

BACKGROUND: The identification and functional analysis of genes that improve tolerance to low potassium stress in S. spontaneum is crucial for breeding sugarcane cultivars with efficient potassium utilization. Calcineurin B-like (CBL) protein is a calcium sensor that interacts with specific CBL-inte...

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Autores principales: Feng, Xiaomin, Wang, Yongjun, Zhang, Nannan, Gao, Shuai, Wu, Jiayun, Liu, Rui, Huang, Yonghong, Zhang, Jisen, Qi, Yongwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383383/
https://www.ncbi.nlm.nih.gov/pubmed/34425748
http://dx.doi.org/10.1186/s12870-021-03175-3
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author Feng, Xiaomin
Wang, Yongjun
Zhang, Nannan
Gao, Shuai
Wu, Jiayun
Liu, Rui
Huang, Yonghong
Zhang, Jisen
Qi, Yongwen
author_facet Feng, Xiaomin
Wang, Yongjun
Zhang, Nannan
Gao, Shuai
Wu, Jiayun
Liu, Rui
Huang, Yonghong
Zhang, Jisen
Qi, Yongwen
author_sort Feng, Xiaomin
collection PubMed
description BACKGROUND: The identification and functional analysis of genes that improve tolerance to low potassium stress in S. spontaneum is crucial for breeding sugarcane cultivars with efficient potassium utilization. Calcineurin B-like (CBL) protein is a calcium sensor that interacts with specific CBL-interacting protein kinases (CIPKs) upon plants’ exposure to various abiotic stresses. RESULTS: In this study, nine CBL genes were identified from S. spontaneum. Phylogenetic analysis of 113 CBLs from 13 representative plants showed gene expansion and strong purifying selection in the CBL family. Analysis of CBL expression patterns revealed that SsCBL01 was the most commonly expressed gene in various tissues at different developmental stages. Expression analysis of SsCBLs under low K(+) stress indicated that potassium deficiency moderately altered the transcription of SsCBLs. Subcellular localization showed that SsCBL01 is a plasma membrane protein and heterologous expression in yeast suggested that, while SsCBL01 alone could not absorb K(+), it positively regulated K(+) absorption mediated by the potassium transporter SsHAK1. CONCLUSIONS: This study provided insights into the evolution of the CBL gene family and preliminarily demonstrated that the plasma membrane protein SsCBL01 was involved in the response to low K(+) stress in S. spontaneum. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03175-3.
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spelling pubmed-83833832021-08-25 Comparative phylogenetic analysis of CBL reveals the gene family evolution and functional divergence in Saccharum spontaneum Feng, Xiaomin Wang, Yongjun Zhang, Nannan Gao, Shuai Wu, Jiayun Liu, Rui Huang, Yonghong Zhang, Jisen Qi, Yongwen BMC Plant Biol Research BACKGROUND: The identification and functional analysis of genes that improve tolerance to low potassium stress in S. spontaneum is crucial for breeding sugarcane cultivars with efficient potassium utilization. Calcineurin B-like (CBL) protein is a calcium sensor that interacts with specific CBL-interacting protein kinases (CIPKs) upon plants’ exposure to various abiotic stresses. RESULTS: In this study, nine CBL genes were identified from S. spontaneum. Phylogenetic analysis of 113 CBLs from 13 representative plants showed gene expansion and strong purifying selection in the CBL family. Analysis of CBL expression patterns revealed that SsCBL01 was the most commonly expressed gene in various tissues at different developmental stages. Expression analysis of SsCBLs under low K(+) stress indicated that potassium deficiency moderately altered the transcription of SsCBLs. Subcellular localization showed that SsCBL01 is a plasma membrane protein and heterologous expression in yeast suggested that, while SsCBL01 alone could not absorb K(+), it positively regulated K(+) absorption mediated by the potassium transporter SsHAK1. CONCLUSIONS: This study provided insights into the evolution of the CBL gene family and preliminarily demonstrated that the plasma membrane protein SsCBL01 was involved in the response to low K(+) stress in S. spontaneum. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03175-3. BioMed Central 2021-08-23 /pmc/articles/PMC8383383/ /pubmed/34425748 http://dx.doi.org/10.1186/s12870-021-03175-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Feng, Xiaomin
Wang, Yongjun
Zhang, Nannan
Gao, Shuai
Wu, Jiayun
Liu, Rui
Huang, Yonghong
Zhang, Jisen
Qi, Yongwen
Comparative phylogenetic analysis of CBL reveals the gene family evolution and functional divergence in Saccharum spontaneum
title Comparative phylogenetic analysis of CBL reveals the gene family evolution and functional divergence in Saccharum spontaneum
title_full Comparative phylogenetic analysis of CBL reveals the gene family evolution and functional divergence in Saccharum spontaneum
title_fullStr Comparative phylogenetic analysis of CBL reveals the gene family evolution and functional divergence in Saccharum spontaneum
title_full_unstemmed Comparative phylogenetic analysis of CBL reveals the gene family evolution and functional divergence in Saccharum spontaneum
title_short Comparative phylogenetic analysis of CBL reveals the gene family evolution and functional divergence in Saccharum spontaneum
title_sort comparative phylogenetic analysis of cbl reveals the gene family evolution and functional divergence in saccharum spontaneum
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383383/
https://www.ncbi.nlm.nih.gov/pubmed/34425748
http://dx.doi.org/10.1186/s12870-021-03175-3
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