Cargando…
Taohong Siwu Decoction Regulates Cell Necrosis and Neuroinflammation in the Rat Middle Cerebral Artery Occlusion Model
Cell necrosis and neuroinflammation play an important role in brain injury induced by ischemic stroke. Previous studies reported that Taohong Siwu decoction (THSWD)can reduce heart muscle cell necrosis and has anti-inflammatory properties. In this study, we investigated the effects of THSWD on cell...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384077/ https://www.ncbi.nlm.nih.gov/pubmed/34447315 http://dx.doi.org/10.3389/fphar.2021.732358 |
_version_ | 1783741847939579904 |
---|---|
author | Wang, Ni Fei, Changyi Chu, Furui Huang, Shi Pan, Lingyu Peng, Daiyin Duan, Xianchun |
author_facet | Wang, Ni Fei, Changyi Chu, Furui Huang, Shi Pan, Lingyu Peng, Daiyin Duan, Xianchun |
author_sort | Wang, Ni |
collection | PubMed |
description | Cell necrosis and neuroinflammation play an important role in brain injury induced by ischemic stroke. Previous studies reported that Taohong Siwu decoction (THSWD)can reduce heart muscle cell necrosis and has anti-inflammatory properties. In this study, we investigated the effects of THSWD on cell necrosis and neuroinflammation in a rat model of middle cerebral artery occlusion (MCAO). Thirty-six male Sprague-Dawley (SD) rats were randomly divided into three groups with 12 rats in each group. They were the sham operation group, MCAO model group, and MCAO + THSWD group. We used ELISA to determine the levels of TNF-α, Mcp-1, and IL-1β inflammatory factors in rat serum, qRT‐PCR to detect the expression of TNF‐α, Mcp‐1 and IL‐1β mRNA in rat brain, and immunohistochemistry to detect the number of microglia and neutrophils in rat brain. qRT-PCR and Western blot were used to detect the mRNA and protein expression levels of IBA-1 and MPO inflammatory factors and the TNF-α/RIP1/RIP3/MLKL pathway in the rat brain and protein expression levels. Compared with the sham operation group, the expression of MCP-1, IL-1β, IBA-1, and MPO inflammatory factors and the TNF-α/RIP1/RIP3/MLKL pathway were significantly upregulated in the MCAO group. Compared with the MCAO group, the expressions of MCP-1, IL-1β, IBA-1, and MPO inflammatory factors and the TNF-α/RIP1/RIP3/MLKL pathway were significantly downregulated in the MCAO + THSWD group. THSWD can reduce the expression levels of MCP-1, IL-1β, IBA-1, and MPO inflammatory factors as well as the TNF-α/RIP1/RIP3/MLKL pathway. Meanwhile, it can reduce the necrosis and inflammation of brain cells after cerebral ischemia, so as to protect the brain tissue of rats. |
format | Online Article Text |
id | pubmed-8384077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83840772021-08-25 Taohong Siwu Decoction Regulates Cell Necrosis and Neuroinflammation in the Rat Middle Cerebral Artery Occlusion Model Wang, Ni Fei, Changyi Chu, Furui Huang, Shi Pan, Lingyu Peng, Daiyin Duan, Xianchun Front Pharmacol Pharmacology Cell necrosis and neuroinflammation play an important role in brain injury induced by ischemic stroke. Previous studies reported that Taohong Siwu decoction (THSWD)can reduce heart muscle cell necrosis and has anti-inflammatory properties. In this study, we investigated the effects of THSWD on cell necrosis and neuroinflammation in a rat model of middle cerebral artery occlusion (MCAO). Thirty-six male Sprague-Dawley (SD) rats were randomly divided into three groups with 12 rats in each group. They were the sham operation group, MCAO model group, and MCAO + THSWD group. We used ELISA to determine the levels of TNF-α, Mcp-1, and IL-1β inflammatory factors in rat serum, qRT‐PCR to detect the expression of TNF‐α, Mcp‐1 and IL‐1β mRNA in rat brain, and immunohistochemistry to detect the number of microglia and neutrophils in rat brain. qRT-PCR and Western blot were used to detect the mRNA and protein expression levels of IBA-1 and MPO inflammatory factors and the TNF-α/RIP1/RIP3/MLKL pathway in the rat brain and protein expression levels. Compared with the sham operation group, the expression of MCP-1, IL-1β, IBA-1, and MPO inflammatory factors and the TNF-α/RIP1/RIP3/MLKL pathway were significantly upregulated in the MCAO group. Compared with the MCAO group, the expressions of MCP-1, IL-1β, IBA-1, and MPO inflammatory factors and the TNF-α/RIP1/RIP3/MLKL pathway were significantly downregulated in the MCAO + THSWD group. THSWD can reduce the expression levels of MCP-1, IL-1β, IBA-1, and MPO inflammatory factors as well as the TNF-α/RIP1/RIP3/MLKL pathway. Meanwhile, it can reduce the necrosis and inflammation of brain cells after cerebral ischemia, so as to protect the brain tissue of rats. Frontiers Media S.A. 2021-08-10 /pmc/articles/PMC8384077/ /pubmed/34447315 http://dx.doi.org/10.3389/fphar.2021.732358 Text en Copyright © 2021 Wang, Fei, Chu, Huang, Pan, Peng and Duan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Wang, Ni Fei, Changyi Chu, Furui Huang, Shi Pan, Lingyu Peng, Daiyin Duan, Xianchun Taohong Siwu Decoction Regulates Cell Necrosis and Neuroinflammation in the Rat Middle Cerebral Artery Occlusion Model |
title | Taohong Siwu Decoction Regulates Cell Necrosis and Neuroinflammation in the Rat Middle Cerebral Artery Occlusion Model |
title_full | Taohong Siwu Decoction Regulates Cell Necrosis and Neuroinflammation in the Rat Middle Cerebral Artery Occlusion Model |
title_fullStr | Taohong Siwu Decoction Regulates Cell Necrosis and Neuroinflammation in the Rat Middle Cerebral Artery Occlusion Model |
title_full_unstemmed | Taohong Siwu Decoction Regulates Cell Necrosis and Neuroinflammation in the Rat Middle Cerebral Artery Occlusion Model |
title_short | Taohong Siwu Decoction Regulates Cell Necrosis and Neuroinflammation in the Rat Middle Cerebral Artery Occlusion Model |
title_sort | taohong siwu decoction regulates cell necrosis and neuroinflammation in the rat middle cerebral artery occlusion model |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384077/ https://www.ncbi.nlm.nih.gov/pubmed/34447315 http://dx.doi.org/10.3389/fphar.2021.732358 |
work_keys_str_mv | AT wangni taohongsiwudecoctionregulatescellnecrosisandneuroinflammationintheratmiddlecerebralarteryocclusionmodel AT feichangyi taohongsiwudecoctionregulatescellnecrosisandneuroinflammationintheratmiddlecerebralarteryocclusionmodel AT chufurui taohongsiwudecoctionregulatescellnecrosisandneuroinflammationintheratmiddlecerebralarteryocclusionmodel AT huangshi taohongsiwudecoctionregulatescellnecrosisandneuroinflammationintheratmiddlecerebralarteryocclusionmodel AT panlingyu taohongsiwudecoctionregulatescellnecrosisandneuroinflammationintheratmiddlecerebralarteryocclusionmodel AT pengdaiyin taohongsiwudecoctionregulatescellnecrosisandneuroinflammationintheratmiddlecerebralarteryocclusionmodel AT duanxianchun taohongsiwudecoctionregulatescellnecrosisandneuroinflammationintheratmiddlecerebralarteryocclusionmodel |