Cargando…
The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus
The vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the production of infectious new progeny virus particles....
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384211/ https://www.ncbi.nlm.nih.gov/pubmed/34428224 http://dx.doi.org/10.1371/journal.pcbi.1009306 |
_version_ | 1783741872865280000 |
---|---|
author | Hill, Sam R. Twarock, Reidun Dykeman, Eric C. |
author_facet | Hill, Sam R. Twarock, Reidun Dykeman, Eric C. |
author_sort | Hill, Sam R. |
collection | PubMed |
description | The vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the production of infectious new progeny virus particles. In the class of single-stranded RNA (ssRNA) viruses, the assembly of the capsid takes place in tandem with packaging of the ssRNA genome in a highly cooperative co-assembly process. In simple ssRNA viruses such as the bacteriophage MS2 and small RNA plant viruses such as STNV, this cooperative process results from multiple interactions between the protein shell and sites in the RNA genome which have been termed packaging signals. Using a stochastic assembly algorithm which includes cooperative interactions between the protein shell and packaging signals in the RNA genome, we demonstrate that highly efficient assembly of STNV capsids arises from a set of simple local rules. Altering the local assembly rules results in different nucleation scenarios with varying assembly efficiencies, which in some cases depend strongly on interactions with RNA packaging signals. Our results provide a potential simple explanation based on local assembly rules for the ability of some ssRNA viruses to spontaneously assemble around charged polymers and other non-viral RNAs in vitro. |
format | Online Article Text |
id | pubmed-8384211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-83842112021-08-25 The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus Hill, Sam R. Twarock, Reidun Dykeman, Eric C. PLoS Comput Biol Research Article The vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the production of infectious new progeny virus particles. In the class of single-stranded RNA (ssRNA) viruses, the assembly of the capsid takes place in tandem with packaging of the ssRNA genome in a highly cooperative co-assembly process. In simple ssRNA viruses such as the bacteriophage MS2 and small RNA plant viruses such as STNV, this cooperative process results from multiple interactions between the protein shell and sites in the RNA genome which have been termed packaging signals. Using a stochastic assembly algorithm which includes cooperative interactions between the protein shell and packaging signals in the RNA genome, we demonstrate that highly efficient assembly of STNV capsids arises from a set of simple local rules. Altering the local assembly rules results in different nucleation scenarios with varying assembly efficiencies, which in some cases depend strongly on interactions with RNA packaging signals. Our results provide a potential simple explanation based on local assembly rules for the ability of some ssRNA viruses to spontaneously assemble around charged polymers and other non-viral RNAs in vitro. Public Library of Science 2021-08-24 /pmc/articles/PMC8384211/ /pubmed/34428224 http://dx.doi.org/10.1371/journal.pcbi.1009306 Text en © 2021 Hill et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hill, Sam R. Twarock, Reidun Dykeman, Eric C. The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus |
title | The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus |
title_full | The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus |
title_fullStr | The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus |
title_full_unstemmed | The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus |
title_short | The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus |
title_sort | impact of local assembly rules on rna packaging in a t = 1 satellite plant virus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384211/ https://www.ncbi.nlm.nih.gov/pubmed/34428224 http://dx.doi.org/10.1371/journal.pcbi.1009306 |
work_keys_str_mv | AT hillsamr theimpactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus AT twarockreidun theimpactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus AT dykemanericc theimpactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus AT hillsamr impactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus AT twarockreidun impactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus AT dykemanericc impactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus |