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Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles
OBJECTIVES: The present study evaluated the antimicrobial effects of fissure sealants containing chitosan nanoparticles. MATERIALS AND METHODS: Antibacterial effect of Master Dent fissure sealant alone and after incorporating chitosan nanoparticles was evaluated on Streptococcus mutans, sanguis, and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384512/ https://www.ncbi.nlm.nih.gov/pubmed/34447437 http://dx.doi.org/10.1155/2021/8975948 |
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author | Hashemi kamangar, Sedighe Sadat Zareian, Houtan Bahador, Abbas Pourhajibagher, Maryam Bashareh, Zahra Valizadeh, Sara |
author_facet | Hashemi kamangar, Sedighe Sadat Zareian, Houtan Bahador, Abbas Pourhajibagher, Maryam Bashareh, Zahra Valizadeh, Sara |
author_sort | Hashemi kamangar, Sedighe Sadat |
collection | PubMed |
description | OBJECTIVES: The present study evaluated the antimicrobial effects of fissure sealants containing chitosan nanoparticles. MATERIALS AND METHODS: Antibacterial effect of Master Dent fissure sealant alone and after incorporating chitosan nanoparticles was evaluated on Streptococcus mutans, sanguis, and Lactobacillus acidophilus. Biofilm growth was evaluated by determining colony counts. Antimicrobial effect was determined on days 3, 15, and 30 by counting microbial colonies using eluted components test. One-way ANOVA, Tukey HSD tests, t test, and two-way ANOVA were used for statistical analyses (α = 0.05). RESULTS: Biofilm inhibition test showed that fissure sealant containing 1 wt.% chitosan decreased colony counts significantly (P < 0.05). Eluted components test with S. mutans and sanguis showed significant decrease in colony counts during the first 15 days in chitosan containing group; however, from day 30, antimicrobial activity decreased noticeably, with no significant difference from control group (P > 0.05). Antimicrobial activity against L. acidophilus was maintained in chitosan group up to 30 days, and decrease in colony counts was significant (P < 0.05). CONCLUSION: According to the results of this study, incorporation of 1 wt.% chitosan into fissure sealant induced an antimicrobial activity. Antibacterial effect on L. acidophilus persisted for longer time (30 days) compared to the two other bacterial species (15 days). |
format | Online Article Text |
id | pubmed-8384512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-83845122021-08-25 Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles Hashemi kamangar, Sedighe Sadat Zareian, Houtan Bahador, Abbas Pourhajibagher, Maryam Bashareh, Zahra Valizadeh, Sara Int J Dent Research Article OBJECTIVES: The present study evaluated the antimicrobial effects of fissure sealants containing chitosan nanoparticles. MATERIALS AND METHODS: Antibacterial effect of Master Dent fissure sealant alone and after incorporating chitosan nanoparticles was evaluated on Streptococcus mutans, sanguis, and Lactobacillus acidophilus. Biofilm growth was evaluated by determining colony counts. Antimicrobial effect was determined on days 3, 15, and 30 by counting microbial colonies using eluted components test. One-way ANOVA, Tukey HSD tests, t test, and two-way ANOVA were used for statistical analyses (α = 0.05). RESULTS: Biofilm inhibition test showed that fissure sealant containing 1 wt.% chitosan decreased colony counts significantly (P < 0.05). Eluted components test with S. mutans and sanguis showed significant decrease in colony counts during the first 15 days in chitosan containing group; however, from day 30, antimicrobial activity decreased noticeably, with no significant difference from control group (P > 0.05). Antimicrobial activity against L. acidophilus was maintained in chitosan group up to 30 days, and decrease in colony counts was significant (P < 0.05). CONCLUSION: According to the results of this study, incorporation of 1 wt.% chitosan into fissure sealant induced an antimicrobial activity. Antibacterial effect on L. acidophilus persisted for longer time (30 days) compared to the two other bacterial species (15 days). Hindawi 2021-08-16 /pmc/articles/PMC8384512/ /pubmed/34447437 http://dx.doi.org/10.1155/2021/8975948 Text en Copyright © 2021 Sedighe Sadat Hashemi kamangar et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hashemi kamangar, Sedighe Sadat Zareian, Houtan Bahador, Abbas Pourhajibagher, Maryam Bashareh, Zahra Valizadeh, Sara Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
title | Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
title_full | Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
title_fullStr | Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
title_full_unstemmed | Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
title_short | Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
title_sort | evaluation of antibacterial effects of fissure sealants containing chitosan nanoparticles |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384512/ https://www.ncbi.nlm.nih.gov/pubmed/34447437 http://dx.doi.org/10.1155/2021/8975948 |
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