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Evaluation of a mechanical lung model to test small animal whole body plethysmography
Whole-body plethysmography (WBP) is an established method to determine physiological parameters and pathophysiological alteration of breathing in animals and animal models of a variety of diseases. Although frequently used, there is ongoing debate about what exactly is measured by whole-body-plethys...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384843/ https://www.ncbi.nlm.nih.gov/pubmed/34429449 http://dx.doi.org/10.1038/s41598-021-96355-y |
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author | Hülsmann, Swen Khan, Amara Hagos, Liya Hindermann, Martin Nägel, Torsten Dullin, Christian |
author_facet | Hülsmann, Swen Khan, Amara Hagos, Liya Hindermann, Martin Nägel, Torsten Dullin, Christian |
author_sort | Hülsmann, Swen |
collection | PubMed |
description | Whole-body plethysmography (WBP) is an established method to determine physiological parameters and pathophysiological alteration of breathing in animals and animal models of a variety of diseases. Although frequently used, there is ongoing debate about what exactly is measured by whole-body-plethysmography and how reliable the data derived from this method are. Here, we designed an artificial lung model that enables a thorough evaluation of different predictions about and around whole-body plethysmography. Using our lung model, we confirmed that during WBP two components contribute to the pressure changes detected in the chamber: (1) the increase in the pressure due to heating and moistening of the air during inspiration, termed conditioning; (2) changes in the chamber pressure that depend on airway resistance. Both components overlap and contribute to the temporal pressure-profile measured in the chamber or across the wall of the chamber, respectively. Our data showed that a precise measurement of the breathing volume appears to be hindered by at least two factors: (1) the unknown relative contribution of each of these two components; (2) not only the air in the inspired volume is conditioned during inspiration, but also air within the residual volume and dead space that is recruited during inspiration. Moreover, our data suggest that the expiratory negative pressure peak that is used to determine the enhanced pause (Penh) parameter is not a measure for airway resistance as such but rather a consequence of the animal’s response to the airway resistance, using forced or active expiration to overcome the resistance by a higher thoracic pressure. |
format | Online Article Text |
id | pubmed-8384843 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83848432021-09-01 Evaluation of a mechanical lung model to test small animal whole body plethysmography Hülsmann, Swen Khan, Amara Hagos, Liya Hindermann, Martin Nägel, Torsten Dullin, Christian Sci Rep Article Whole-body plethysmography (WBP) is an established method to determine physiological parameters and pathophysiological alteration of breathing in animals and animal models of a variety of diseases. Although frequently used, there is ongoing debate about what exactly is measured by whole-body-plethysmography and how reliable the data derived from this method are. Here, we designed an artificial lung model that enables a thorough evaluation of different predictions about and around whole-body plethysmography. Using our lung model, we confirmed that during WBP two components contribute to the pressure changes detected in the chamber: (1) the increase in the pressure due to heating and moistening of the air during inspiration, termed conditioning; (2) changes in the chamber pressure that depend on airway resistance. Both components overlap and contribute to the temporal pressure-profile measured in the chamber or across the wall of the chamber, respectively. Our data showed that a precise measurement of the breathing volume appears to be hindered by at least two factors: (1) the unknown relative contribution of each of these two components; (2) not only the air in the inspired volume is conditioned during inspiration, but also air within the residual volume and dead space that is recruited during inspiration. Moreover, our data suggest that the expiratory negative pressure peak that is used to determine the enhanced pause (Penh) parameter is not a measure for airway resistance as such but rather a consequence of the animal’s response to the airway resistance, using forced or active expiration to overcome the resistance by a higher thoracic pressure. Nature Publishing Group UK 2021-08-24 /pmc/articles/PMC8384843/ /pubmed/34429449 http://dx.doi.org/10.1038/s41598-021-96355-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hülsmann, Swen Khan, Amara Hagos, Liya Hindermann, Martin Nägel, Torsten Dullin, Christian Evaluation of a mechanical lung model to test small animal whole body plethysmography |
title | Evaluation of a mechanical lung model to test small animal whole body plethysmography |
title_full | Evaluation of a mechanical lung model to test small animal whole body plethysmography |
title_fullStr | Evaluation of a mechanical lung model to test small animal whole body plethysmography |
title_full_unstemmed | Evaluation of a mechanical lung model to test small animal whole body plethysmography |
title_short | Evaluation of a mechanical lung model to test small animal whole body plethysmography |
title_sort | evaluation of a mechanical lung model to test small animal whole body plethysmography |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384843/ https://www.ncbi.nlm.nih.gov/pubmed/34429449 http://dx.doi.org/10.1038/s41598-021-96355-y |
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