Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR(46) azole resistance gene

Infections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp51A gene....

Descripción completa

Detalles Bibliográficos
Autores principales: Trabasso, Plinio, Matsuzawa, Tetsuhiro, Arai, Teppei, Hagiwara, Daisuke, Mikami, Yuzuru, Moretti, Maria Luiza, Watanabe, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384855/
https://www.ncbi.nlm.nih.gov/pubmed/34429488
http://dx.doi.org/10.1038/s41598-021-96651-7
_version_ 1783741983209029632
author Trabasso, Plinio
Matsuzawa, Tetsuhiro
Arai, Teppei
Hagiwara, Daisuke
Mikami, Yuzuru
Moretti, Maria Luiza
Watanabe, Akira
author_facet Trabasso, Plinio
Matsuzawa, Tetsuhiro
Arai, Teppei
Hagiwara, Daisuke
Mikami, Yuzuru
Moretti, Maria Luiza
Watanabe, Akira
author_sort Trabasso, Plinio
collection PubMed
description Infections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp51A gene. Loop-mediated isothermal amplification (LAMP) is a widely used nucleic acid amplification system that is fast and specific. Here we describe a LAMP assay method to detect the 46 bp tandem repeat insertion in the cyp51A gene promoter region based on novel LAMP primer sets. It also differentiated strains with TR(46) tandem repeats from those with TR(34) tandem repeats. These results showed this TR(46)-LAMP method is specific, rapid, and provides crucial insights to develop novel antifungal therapeutic strategies against severe fungal infections due to A. fumigatus with TR(46) tandem repeats.
format Online
Article
Text
id pubmed-8384855
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-83848552021-09-01 Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR(46) azole resistance gene Trabasso, Plinio Matsuzawa, Tetsuhiro Arai, Teppei Hagiwara, Daisuke Mikami, Yuzuru Moretti, Maria Luiza Watanabe, Akira Sci Rep Article Infections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp51A gene. Loop-mediated isothermal amplification (LAMP) is a widely used nucleic acid amplification system that is fast and specific. Here we describe a LAMP assay method to detect the 46 bp tandem repeat insertion in the cyp51A gene promoter region based on novel LAMP primer sets. It also differentiated strains with TR(46) tandem repeats from those with TR(34) tandem repeats. These results showed this TR(46)-LAMP method is specific, rapid, and provides crucial insights to develop novel antifungal therapeutic strategies against severe fungal infections due to A. fumigatus with TR(46) tandem repeats. Nature Publishing Group UK 2021-08-24 /pmc/articles/PMC8384855/ /pubmed/34429488 http://dx.doi.org/10.1038/s41598-021-96651-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Trabasso, Plinio
Matsuzawa, Tetsuhiro
Arai, Teppei
Hagiwara, Daisuke
Mikami, Yuzuru
Moretti, Maria Luiza
Watanabe, Akira
Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR(46) azole resistance gene
title Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR(46) azole resistance gene
title_full Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR(46) azole resistance gene
title_fullStr Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR(46) azole resistance gene
title_full_unstemmed Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR(46) azole resistance gene
title_short Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR(46) azole resistance gene
title_sort development and validation of lamp primer sets for rapid identification of aspergillus fumigatus carrying the cyp51a tr(46) azole resistance gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384855/
https://www.ncbi.nlm.nih.gov/pubmed/34429488
http://dx.doi.org/10.1038/s41598-021-96651-7
work_keys_str_mv AT trabassoplinio developmentandvalidationoflampprimersetsforrapididentificationofaspergillusfumigatuscarryingthecyp51atr46azoleresistancegene
AT matsuzawatetsuhiro developmentandvalidationoflampprimersetsforrapididentificationofaspergillusfumigatuscarryingthecyp51atr46azoleresistancegene
AT araiteppei developmentandvalidationoflampprimersetsforrapididentificationofaspergillusfumigatuscarryingthecyp51atr46azoleresistancegene
AT hagiwaradaisuke developmentandvalidationoflampprimersetsforrapididentificationofaspergillusfumigatuscarryingthecyp51atr46azoleresistancegene
AT mikamiyuzuru developmentandvalidationoflampprimersetsforrapididentificationofaspergillusfumigatuscarryingthecyp51atr46azoleresistancegene
AT morettimarialuiza developmentandvalidationoflampprimersetsforrapididentificationofaspergillusfumigatuscarryingthecyp51atr46azoleresistancegene
AT watanabeakira developmentandvalidationoflampprimersetsforrapididentificationofaspergillusfumigatuscarryingthecyp51atr46azoleresistancegene

Ejemplares similares