Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag

Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury. Here, we have adapted the Cleavage Under Target and Tagmentation technology to the mapping of the chromatin landscape and transcription factor binding in 50...

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Detalles Bibliográficos
Autores principales: Li, Yuefeng, Nakka, Kiran, Olender, Thomas, Gingras-Gelinas, Philippe, Wong, Matthew Man-Kin, Robinson, Daniel C.L., Bandukwala, Hina, Palii, Carmen G., Neyret, Odile, Brand, Marjorie, Blais, Alexandre, Dilworth, F. Jeffrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384913/
https://www.ncbi.nlm.nih.gov/pubmed/34467227
http://dx.doi.org/10.1016/j.xpro.2021.100751
Descripción
Sumario:Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury. Here, we have adapted the Cleavage Under Target and Tagmentation technology to the mapping of the chromatin landscape and transcription factor binding in 50,000 activated MuSCs isolated from injured mouse hindlimb muscles. We have applied this same approach to human CD34(+) hematopoietic stem and progenitor cells. This protocol could be adapted to any rare stem cell population. For complete details on the use and execution of this protocol, please refer to Robinson et al. (2021).

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