Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag

Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury. Here, we have adapted the Cleavage Under Target and Tagmentation technology to the mapping of the chromatin landscape and transcription factor binding in 50...

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Autores principales: Li, Yuefeng, Nakka, Kiran, Olender, Thomas, Gingras-Gelinas, Philippe, Wong, Matthew Man-Kin, Robinson, Daniel C.L., Bandukwala, Hina, Palii, Carmen G., Neyret, Odile, Brand, Marjorie, Blais, Alexandre, Dilworth, F. Jeffrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384913/
https://www.ncbi.nlm.nih.gov/pubmed/34467227
http://dx.doi.org/10.1016/j.xpro.2021.100751
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author Li, Yuefeng
Nakka, Kiran
Olender, Thomas
Gingras-Gelinas, Philippe
Wong, Matthew Man-Kin
Robinson, Daniel C.L.
Bandukwala, Hina
Palii, Carmen G.
Neyret, Odile
Brand, Marjorie
Blais, Alexandre
Dilworth, F. Jeffrey
author_facet Li, Yuefeng
Nakka, Kiran
Olender, Thomas
Gingras-Gelinas, Philippe
Wong, Matthew Man-Kin
Robinson, Daniel C.L.
Bandukwala, Hina
Palii, Carmen G.
Neyret, Odile
Brand, Marjorie
Blais, Alexandre
Dilworth, F. Jeffrey
author_sort Li, Yuefeng
collection PubMed
description Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury. Here, we have adapted the Cleavage Under Target and Tagmentation technology to the mapping of the chromatin landscape and transcription factor binding in 50,000 activated MuSCs isolated from injured mouse hindlimb muscles. We have applied this same approach to human CD34(+) hematopoietic stem and progenitor cells. This protocol could be adapted to any rare stem cell population. For complete details on the use and execution of this protocol, please refer to Robinson et al. (2021).
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spelling pubmed-83849132021-08-30 Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag Li, Yuefeng Nakka, Kiran Olender, Thomas Gingras-Gelinas, Philippe Wong, Matthew Man-Kin Robinson, Daniel C.L. Bandukwala, Hina Palii, Carmen G. Neyret, Odile Brand, Marjorie Blais, Alexandre Dilworth, F. Jeffrey STAR Protoc Protocol Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury. Here, we have adapted the Cleavage Under Target and Tagmentation technology to the mapping of the chromatin landscape and transcription factor binding in 50,000 activated MuSCs isolated from injured mouse hindlimb muscles. We have applied this same approach to human CD34(+) hematopoietic stem and progenitor cells. This protocol could be adapted to any rare stem cell population. For complete details on the use and execution of this protocol, please refer to Robinson et al. (2021). Elsevier 2021-08-19 /pmc/articles/PMC8384913/ /pubmed/34467227 http://dx.doi.org/10.1016/j.xpro.2021.100751 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Li, Yuefeng
Nakka, Kiran
Olender, Thomas
Gingras-Gelinas, Philippe
Wong, Matthew Man-Kin
Robinson, Daniel C.L.
Bandukwala, Hina
Palii, Carmen G.
Neyret, Odile
Brand, Marjorie
Blais, Alexandre
Dilworth, F. Jeffrey
Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag
title Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag
title_full Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag
title_fullStr Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag
title_full_unstemmed Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag
title_short Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag
title_sort chromatin and transcription factor profiling in rare stem cell populations using cut&tag
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384913/
https://www.ncbi.nlm.nih.gov/pubmed/34467227
http://dx.doi.org/10.1016/j.xpro.2021.100751
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