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Quantification of adipocyte numbers in transgenic mice via the Cre-LoxP recombination sites

This protocol describes a method to assess adipocyte numbers within a specific depot based on their inducible genomic label. By extracting DNA from a complete adipose tissue depot stemming from two transgenic mouse lines (Adipoq-CreERT2 x ROSA26-tdRFP and Ucp1-CreERT2 x ROSA26-tdRFP), the number of...

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Detalles Bibliográficos
Autores principales: Moser, Caroline, Straub, Leon G., Rachamin, Yael, Wolfrum, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385114/
https://www.ncbi.nlm.nih.gov/pubmed/34467230
http://dx.doi.org/10.1016/j.xpro.2021.100761