Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages

Dynamic changes in histone modifications mediated by Polycomb group proteins can be indicative of the transition of gene repression mode during development. Here, we present methods for the isolation of mouse neocortical neural progenitor-stem cells (NPCs) and their culture, followed by chromatin im...

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Autores principales: Tsuboi, Masafumi, Gotoh, Yukiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385115/
https://www.ncbi.nlm.nih.gov/pubmed/34467231
http://dx.doi.org/10.1016/j.xpro.2021.100763
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author Tsuboi, Masafumi
Gotoh, Yukiko
author_facet Tsuboi, Masafumi
Gotoh, Yukiko
author_sort Tsuboi, Masafumi
collection PubMed
description Dynamic changes in histone modifications mediated by Polycomb group proteins can be indicative of the transition of gene repression mode during development. Here, we present methods for the isolation of mouse neocortical neural progenitor-stem cells (NPCs) and their culture, followed by chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) techniques to examine changes in histone H2A ubiquitination patterns at various developmental stages. This protocol can be applied for both in vitro NPCs and NPCs directly isolated from mouse neocortices. For complete details on the use and execution of this protocol, please refer to (Tsuboi et al., 2018).
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spelling pubmed-83851152021-08-30 Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages Tsuboi, Masafumi Gotoh, Yukiko STAR Protoc Protocol Dynamic changes in histone modifications mediated by Polycomb group proteins can be indicative of the transition of gene repression mode during development. Here, we present methods for the isolation of mouse neocortical neural progenitor-stem cells (NPCs) and their culture, followed by chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) techniques to examine changes in histone H2A ubiquitination patterns at various developmental stages. This protocol can be applied for both in vitro NPCs and NPCs directly isolated from mouse neocortices. For complete details on the use and execution of this protocol, please refer to (Tsuboi et al., 2018). Elsevier 2021-08-19 /pmc/articles/PMC8385115/ /pubmed/34467231 http://dx.doi.org/10.1016/j.xpro.2021.100763 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Tsuboi, Masafumi
Gotoh, Yukiko
Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages
title Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages
title_full Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages
title_fullStr Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages
title_full_unstemmed Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages
title_short Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages
title_sort analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385115/
https://www.ncbi.nlm.nih.gov/pubmed/34467231
http://dx.doi.org/10.1016/j.xpro.2021.100763
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