Implementing fluorescence enhancement, quenching, and FRET for investigating flap endonuclease 1 enzymatic reaction at the single-molecule level

Flap endonuclease 1 (FEN1) is an important component of the intricate molecular machinery for DNA replication and repair. FEN1 is a structure-specific 5′ nuclease that cleaves nascent single-stranded 5′ flaps during the maturation of Okazaki fragments. Here, we review our research primarily applying...

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Detalles Bibliográficos
Autores principales: Sobhy, Mohamed A., Tehseen, Muhammad, Takahashi, Masateru, Bralić, Amer, De Biasio, Alfredo, Hamdan, Samir M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385120/
https://www.ncbi.nlm.nih.gov/pubmed/34471492
http://dx.doi.org/10.1016/j.csbj.2021.07.029

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