Cargando…
Evaluation of P22 ELISA for the Detection of Mycobacterium bovis-Specific Antibody in the Oral Fluid of Goats
The ante-mortem diagnosis of tuberculosis (TB) in ruminants is based mainly on the intradermal tuberculin test and the IFN-γ assay. Antibody (Ab)-based tests have emerged as potential tools for the detection of TB infected animals using serum, plasma, or even milk samples. Oral fluids have also been...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385241/ https://www.ncbi.nlm.nih.gov/pubmed/34458351 http://dx.doi.org/10.3389/fvets.2021.674636 |
_version_ | 1783742052734861312 |
---|---|
author | Ortega, Javier Infantes-Lorenzo, José A. Bezos, Javier Roy, Álvaro de Juan, Lucia Romero, Beatriz Moreno, Inmaculada Gómez-Buendía, Alberto Agulló-Ros, Irene Domínguez, Lucas Domínguez, Mercedes |
author_facet | Ortega, Javier Infantes-Lorenzo, José A. Bezos, Javier Roy, Álvaro de Juan, Lucia Romero, Beatriz Moreno, Inmaculada Gómez-Buendía, Alberto Agulló-Ros, Irene Domínguez, Lucas Domínguez, Mercedes |
author_sort | Ortega, Javier |
collection | PubMed |
description | The ante-mortem diagnosis of tuberculosis (TB) in ruminants is based mainly on the intradermal tuberculin test and the IFN-γ assay. Antibody (Ab)-based tests have emerged as potential tools for the detection of TB infected animals using serum, plasma, or even milk samples. Oral fluids have also been evaluated as alternative samples with which to detect specific Abs against Mycobacterium bovis in pigs or wild boars, but not in ruminants. The objective of this study was, therefore, to evaluate the performance of an in house-ELISA for TB diagnosis (P22 ELISA) in goats as an experimental model for the diagnosis of TB using oral fluid samples. Oral fluid samples from 64 goats from a TB-infected herd (n = 197) and all the animals from a TB-free herd (n = 113) were analyzed using the P22 ELISA. The estimated sensitivity (Se) and specificity (Sp) were 34.4% (95% CI: 22.4–45.6) and 100% (95% CI: 97.4–100), respectively. The optimal cut-off point was set at 100% according to the ROC analysis. Those animals with a higher level of Abs in their oral fluid attained a higher lesion score (p = 0.018). In fact, when taking into account only the setting of the animals with severe lesions (n = 16), the ELISA showed a Se of 75% (95% CI: 53.7–96.2). Results of the present study suggest that the P22 ELISA is highly specific but has a limited value detecting infected animals in oral fluid samples. Nevertheless, its performance is significantly higher in the presence of severe lesions. |
format | Online Article Text |
id | pubmed-8385241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83852412021-08-26 Evaluation of P22 ELISA for the Detection of Mycobacterium bovis-Specific Antibody in the Oral Fluid of Goats Ortega, Javier Infantes-Lorenzo, José A. Bezos, Javier Roy, Álvaro de Juan, Lucia Romero, Beatriz Moreno, Inmaculada Gómez-Buendía, Alberto Agulló-Ros, Irene Domínguez, Lucas Domínguez, Mercedes Front Vet Sci Veterinary Science The ante-mortem diagnosis of tuberculosis (TB) in ruminants is based mainly on the intradermal tuberculin test and the IFN-γ assay. Antibody (Ab)-based tests have emerged as potential tools for the detection of TB infected animals using serum, plasma, or even milk samples. Oral fluids have also been evaluated as alternative samples with which to detect specific Abs against Mycobacterium bovis in pigs or wild boars, but not in ruminants. The objective of this study was, therefore, to evaluate the performance of an in house-ELISA for TB diagnosis (P22 ELISA) in goats as an experimental model for the diagnosis of TB using oral fluid samples. Oral fluid samples from 64 goats from a TB-infected herd (n = 197) and all the animals from a TB-free herd (n = 113) were analyzed using the P22 ELISA. The estimated sensitivity (Se) and specificity (Sp) were 34.4% (95% CI: 22.4–45.6) and 100% (95% CI: 97.4–100), respectively. The optimal cut-off point was set at 100% according to the ROC analysis. Those animals with a higher level of Abs in their oral fluid attained a higher lesion score (p = 0.018). In fact, when taking into account only the setting of the animals with severe lesions (n = 16), the ELISA showed a Se of 75% (95% CI: 53.7–96.2). Results of the present study suggest that the P22 ELISA is highly specific but has a limited value detecting infected animals in oral fluid samples. Nevertheless, its performance is significantly higher in the presence of severe lesions. Frontiers Media S.A. 2021-08-11 /pmc/articles/PMC8385241/ /pubmed/34458351 http://dx.doi.org/10.3389/fvets.2021.674636 Text en Copyright © 2021 Ortega, Infantes-Lorenzo, Bezos, Roy, de Juan, Romero, Moreno, Gómez-Buendía, Agulló-Ros, Domínguez and Domínguez. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Ortega, Javier Infantes-Lorenzo, José A. Bezos, Javier Roy, Álvaro de Juan, Lucia Romero, Beatriz Moreno, Inmaculada Gómez-Buendía, Alberto Agulló-Ros, Irene Domínguez, Lucas Domínguez, Mercedes Evaluation of P22 ELISA for the Detection of Mycobacterium bovis-Specific Antibody in the Oral Fluid of Goats |
title | Evaluation of P22 ELISA for the Detection of Mycobacterium bovis-Specific Antibody in the Oral Fluid of Goats |
title_full | Evaluation of P22 ELISA for the Detection of Mycobacterium bovis-Specific Antibody in the Oral Fluid of Goats |
title_fullStr | Evaluation of P22 ELISA for the Detection of Mycobacterium bovis-Specific Antibody in the Oral Fluid of Goats |
title_full_unstemmed | Evaluation of P22 ELISA for the Detection of Mycobacterium bovis-Specific Antibody in the Oral Fluid of Goats |
title_short | Evaluation of P22 ELISA for the Detection of Mycobacterium bovis-Specific Antibody in the Oral Fluid of Goats |
title_sort | evaluation of p22 elisa for the detection of mycobacterium bovis-specific antibody in the oral fluid of goats |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385241/ https://www.ncbi.nlm.nih.gov/pubmed/34458351 http://dx.doi.org/10.3389/fvets.2021.674636 |
work_keys_str_mv | AT ortegajavier evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats AT infanteslorenzojosea evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats AT bezosjavier evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats AT royalvaro evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats AT dejuanlucia evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats AT romerobeatriz evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats AT morenoinmaculada evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats AT gomezbuendiaalberto evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats AT agullorosirene evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats AT dominguezlucas evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats AT dominguezmercedes evaluationofp22elisaforthedetectionofmycobacteriumbovisspecificantibodyintheoralfluidofgoats |