Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China

Chicken infectious anemia virus (CIAV) mainly infects chickens and causes immunosuppression. In this study, a CIAV isolate, designated as Pigeon-CIAV-1906, was efficiently isolated from two sick pigeons by inoculating the samples into MSB1 cells. The genome of Pigeon-CIAV-1906 was amplified by PCR a...

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Autores principales: Shao, Hongxia, Li, Jinzhi, Yuan, Huisha, Ji, Lifei, Zhang, Jun, Jin, Wenjie, Qian, Kun, Ye, Jianqiang, Qin, Aijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Veterinary Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385301/
https://www.ncbi.nlm.nih.gov/pubmed/34458350
http://dx.doi.org/10.3389/fvets.2021.669154
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author Shao, Hongxia
Li, Jinzhi
Yuan, Huisha
Ji, Lifei
Zhang, Jun
Jin, Wenjie
Qian, Kun
Ye, Jianqiang
Qin, Aijian
author_facet Shao, Hongxia
Li, Jinzhi
Yuan, Huisha
Ji, Lifei
Zhang, Jun
Jin, Wenjie
Qian, Kun
Ye, Jianqiang
Qin, Aijian
author_sort Shao, Hongxia
collection PubMed
description Chicken infectious anemia virus (CIAV) mainly infects chickens and causes immunosuppression. In this study, a CIAV isolate, designated as Pigeon-CIAV-1906, was efficiently isolated from two sick pigeons by inoculating the samples into MSB1 cells. The genome of Pigeon-CIAV-1906 was amplified by PCR and analyzed. The genome size of Pigeon-CIAV-1906 was 2,298 bp with the highest homology (99.5%) to Jilin strain (JL14023) and the lowest homology (91.5%) to Brazil strain (KY024579), which phylogenetically clustered into Group A. Notably, several amino acids such as 139K and 394Q related with high virulence were found in the VP1 of Pigeon-CIAV-1906. The isolation of Pigeon-CIAV-1906 and its molecular characteristics provide evidence for the cross-transmission of CIAV from chicken to pigeon and give novel insights into the molecular epidemiology of CIAV.
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spelling pubmed-83853012021-08-26 Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China Shao, Hongxia Li, Jinzhi Yuan, Huisha Ji, Lifei Zhang, Jun Jin, Wenjie Qian, Kun Ye, Jianqiang Qin, Aijian Front Vet Sci Veterinary Science Chicken infectious anemia virus (CIAV) mainly infects chickens and causes immunosuppression. In this study, a CIAV isolate, designated as Pigeon-CIAV-1906, was efficiently isolated from two sick pigeons by inoculating the samples into MSB1 cells. The genome of Pigeon-CIAV-1906 was amplified by PCR and analyzed. The genome size of Pigeon-CIAV-1906 was 2,298 bp with the highest homology (99.5%) to Jilin strain (JL14023) and the lowest homology (91.5%) to Brazil strain (KY024579), which phylogenetically clustered into Group A. Notably, several amino acids such as 139K and 394Q related with high virulence were found in the VP1 of Pigeon-CIAV-1906. The isolation of Pigeon-CIAV-1906 and its molecular characteristics provide evidence for the cross-transmission of CIAV from chicken to pigeon and give novel insights into the molecular epidemiology of CIAV. Frontiers Media S.A. 2021-08-11 /pmc/articles/PMC8385301/ /pubmed/34458350 http://dx.doi.org/10.3389/fvets.2021.669154 Text en Copyright © 2021 Shao, Li, Yuan, Ji, Zhang, Jin, Qian, Ye and Qin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Shao, Hongxia
Li, Jinzhi
Yuan, Huisha
Ji, Lifei
Zhang, Jun
Jin, Wenjie
Qian, Kun
Ye, Jianqiang
Qin, Aijian
Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China
title Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China
title_full Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China
title_fullStr Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China
title_full_unstemmed Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China
title_short Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China
title_sort isolation and molecular characteristics of a ciav isolate from pigeons, china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385301/
https://www.ncbi.nlm.nih.gov/pubmed/34458350
http://dx.doi.org/10.3389/fvets.2021.669154
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