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Localization of enteroviral RNA within the pancreas in donors with T1D and T1D-associated autoantibodies
Enteroviral infections have been associated with autoimmunity and type 1 diabetes (T1D), but reliable methods to ascertain localization of single infected cells in the pancreas were missing. Using a single-molecule-based fluorescent in situ hybridization (smFISH) method, we detected increased virus...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385321/ https://www.ncbi.nlm.nih.gov/pubmed/34467248 http://dx.doi.org/10.1016/j.xcrm.2021.100371 |
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author | Geravandi, Shirin Richardson, Sarah Pugliese, Alberto Maedler, Kathrin |
author_facet | Geravandi, Shirin Richardson, Sarah Pugliese, Alberto Maedler, Kathrin |
author_sort | Geravandi, Shirin |
collection | PubMed |
description | Enteroviral infections have been associated with autoimmunity and type 1 diabetes (T1D), but reliable methods to ascertain localization of single infected cells in the pancreas were missing. Using a single-molecule-based fluorescent in situ hybridization (smFISH) method, we detected increased virus infection in pancreases from organ donors with T1D and with disease-associated autoantibodies (AAb(+)). Although virus-positive β cells are found at higher frequency in T1D pancreases, compared to control donors, but are scarce, most virus-positive cells are scattered in the exocrine pancreas. Augmented CD45(+) lymphocytes in T1D pancreases show virus positivity or localization in close proximity to virus-positive cells. Many more infected cells were also found in spleens from T1D donors. The overall increased proportion of virus-positive cells in the pancreas of AAb(+) and T1D organ donors suggests that enteroviruses are associated with immune cell infiltration, autoimmunity, and β cell destruction in both preclinical and diagnosed T1D. |
format | Online Article Text |
id | pubmed-8385321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-83853212021-08-30 Localization of enteroviral RNA within the pancreas in donors with T1D and T1D-associated autoantibodies Geravandi, Shirin Richardson, Sarah Pugliese, Alberto Maedler, Kathrin Cell Rep Med Report Enteroviral infections have been associated with autoimmunity and type 1 diabetes (T1D), but reliable methods to ascertain localization of single infected cells in the pancreas were missing. Using a single-molecule-based fluorescent in situ hybridization (smFISH) method, we detected increased virus infection in pancreases from organ donors with T1D and with disease-associated autoantibodies (AAb(+)). Although virus-positive β cells are found at higher frequency in T1D pancreases, compared to control donors, but are scarce, most virus-positive cells are scattered in the exocrine pancreas. Augmented CD45(+) lymphocytes in T1D pancreases show virus positivity or localization in close proximity to virus-positive cells. Many more infected cells were also found in spleens from T1D donors. The overall increased proportion of virus-positive cells in the pancreas of AAb(+) and T1D organ donors suggests that enteroviruses are associated with immune cell infiltration, autoimmunity, and β cell destruction in both preclinical and diagnosed T1D. Elsevier 2021-08-17 /pmc/articles/PMC8385321/ /pubmed/34467248 http://dx.doi.org/10.1016/j.xcrm.2021.100371 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Report Geravandi, Shirin Richardson, Sarah Pugliese, Alberto Maedler, Kathrin Localization of enteroviral RNA within the pancreas in donors with T1D and T1D-associated autoantibodies |
title | Localization of enteroviral RNA within the pancreas in donors with T1D and T1D-associated autoantibodies |
title_full | Localization of enteroviral RNA within the pancreas in donors with T1D and T1D-associated autoantibodies |
title_fullStr | Localization of enteroviral RNA within the pancreas in donors with T1D and T1D-associated autoantibodies |
title_full_unstemmed | Localization of enteroviral RNA within the pancreas in donors with T1D and T1D-associated autoantibodies |
title_short | Localization of enteroviral RNA within the pancreas in donors with T1D and T1D-associated autoantibodies |
title_sort | localization of enteroviral rna within the pancreas in donors with t1d and t1d-associated autoantibodies |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385321/ https://www.ncbi.nlm.nih.gov/pubmed/34467248 http://dx.doi.org/10.1016/j.xcrm.2021.100371 |
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