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A Missense Mutation in the MYBPH Gene Is Associated With Abdominal Fat Traits in Meat-Type Chickens

Chicken is an important source of protein for human nutrition and a model system for growth and developmental biology. Although the genetic architecture of quantitative traits in meat-type chickens has been the subject of ongoing investigation, the identification of mutations associated with carcass...

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Autores principales: Trevisoli, Priscila Anchieta, Moreira, Gabriel Costa Monteiro, Boschiero, Clarissa, Cesar, Aline Silva Mello, Petrini, Juliana, Margarido, Gabriel Rodrigues Alves, Ledur, Mônica Corrêa, Mourão, Gerson Barreto, Garrick, Dorian, Coutinho, Luiz Lehmann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386115/
https://www.ncbi.nlm.nih.gov/pubmed/34456973
http://dx.doi.org/10.3389/fgene.2021.698163
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author Trevisoli, Priscila Anchieta
Moreira, Gabriel Costa Monteiro
Boschiero, Clarissa
Cesar, Aline Silva Mello
Petrini, Juliana
Margarido, Gabriel Rodrigues Alves
Ledur, Mônica Corrêa
Mourão, Gerson Barreto
Garrick, Dorian
Coutinho, Luiz Lehmann
author_facet Trevisoli, Priscila Anchieta
Moreira, Gabriel Costa Monteiro
Boschiero, Clarissa
Cesar, Aline Silva Mello
Petrini, Juliana
Margarido, Gabriel Rodrigues Alves
Ledur, Mônica Corrêa
Mourão, Gerson Barreto
Garrick, Dorian
Coutinho, Luiz Lehmann
author_sort Trevisoli, Priscila Anchieta
collection PubMed
description Chicken is an important source of protein for human nutrition and a model system for growth and developmental biology. Although the genetic architecture of quantitative traits in meat-type chickens has been the subject of ongoing investigation, the identification of mutations associated with carcass traits of economic interest remains challenging. Therefore, our aim was to identify predicted deleterious mutation, which potentially affects protein function, and test if they were associated with carcass traits in chickens. For that, we performed a genome-wide association analysis (GWAS) for breast, thigh and drumstick traits in meat-type chickens and detected 19 unique quantitative trait loci (QTL). We then used: (1) the identified windows; (2) QTL for abdominal fat detected in a previous study with the same population and (3) previously obtained whole genome sequence data, to identify 18 predicted deleterious single nucleotide polymorphisms (SNPs) in those QTL for further association with breast, thigh, drumstick and abdominal fat traits. Using the additive model, a predicted deleterious SNP c.482C > T (SIFT score of 0.4) was associated (p-value < 0.05) with abdominal fat weight and percentage. This SNP is in the second exon of the MYBPH gene, and its allele frequency deviates from Hardy–Weinberg equilibrium. In conclusion, our study provides evidence that the c.482C > T SNP in the MYBPH gene is a putative causal mutation for fat deposition in meat-type chickens.
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spelling pubmed-83861152021-08-26 A Missense Mutation in the MYBPH Gene Is Associated With Abdominal Fat Traits in Meat-Type Chickens Trevisoli, Priscila Anchieta Moreira, Gabriel Costa Monteiro Boschiero, Clarissa Cesar, Aline Silva Mello Petrini, Juliana Margarido, Gabriel Rodrigues Alves Ledur, Mônica Corrêa Mourão, Gerson Barreto Garrick, Dorian Coutinho, Luiz Lehmann Front Genet Genetics Chicken is an important source of protein for human nutrition and a model system for growth and developmental biology. Although the genetic architecture of quantitative traits in meat-type chickens has been the subject of ongoing investigation, the identification of mutations associated with carcass traits of economic interest remains challenging. Therefore, our aim was to identify predicted deleterious mutation, which potentially affects protein function, and test if they were associated with carcass traits in chickens. For that, we performed a genome-wide association analysis (GWAS) for breast, thigh and drumstick traits in meat-type chickens and detected 19 unique quantitative trait loci (QTL). We then used: (1) the identified windows; (2) QTL for abdominal fat detected in a previous study with the same population and (3) previously obtained whole genome sequence data, to identify 18 predicted deleterious single nucleotide polymorphisms (SNPs) in those QTL for further association with breast, thigh, drumstick and abdominal fat traits. Using the additive model, a predicted deleterious SNP c.482C > T (SIFT score of 0.4) was associated (p-value < 0.05) with abdominal fat weight and percentage. This SNP is in the second exon of the MYBPH gene, and its allele frequency deviates from Hardy–Weinberg equilibrium. In conclusion, our study provides evidence that the c.482C > T SNP in the MYBPH gene is a putative causal mutation for fat deposition in meat-type chickens. Frontiers Media S.A. 2021-08-11 /pmc/articles/PMC8386115/ /pubmed/34456973 http://dx.doi.org/10.3389/fgene.2021.698163 Text en Copyright © 2021 Trevisoli, Moreira, Boschiero, Cesar, Petrini, Margarido, Ledur, Mourão, Garrick and Coutinho. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Trevisoli, Priscila Anchieta
Moreira, Gabriel Costa Monteiro
Boschiero, Clarissa
Cesar, Aline Silva Mello
Petrini, Juliana
Margarido, Gabriel Rodrigues Alves
Ledur, Mônica Corrêa
Mourão, Gerson Barreto
Garrick, Dorian
Coutinho, Luiz Lehmann
A Missense Mutation in the MYBPH Gene Is Associated With Abdominal Fat Traits in Meat-Type Chickens
title A Missense Mutation in the MYBPH Gene Is Associated With Abdominal Fat Traits in Meat-Type Chickens
title_full A Missense Mutation in the MYBPH Gene Is Associated With Abdominal Fat Traits in Meat-Type Chickens
title_fullStr A Missense Mutation in the MYBPH Gene Is Associated With Abdominal Fat Traits in Meat-Type Chickens
title_full_unstemmed A Missense Mutation in the MYBPH Gene Is Associated With Abdominal Fat Traits in Meat-Type Chickens
title_short A Missense Mutation in the MYBPH Gene Is Associated With Abdominal Fat Traits in Meat-Type Chickens
title_sort missense mutation in the mybph gene is associated with abdominal fat traits in meat-type chickens
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386115/
https://www.ncbi.nlm.nih.gov/pubmed/34456973
http://dx.doi.org/10.3389/fgene.2021.698163
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