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Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells

Neoadjuvant chemoradiotherapy has been widely used in the treatment of locally advanced rectal cancer due to the excellent advantages of irradiation in cancer therapy. Unfortunately, not every patient can benefit from this treatment, therefore, it is of great significance to explore biomarkers that...

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Autores principales: Wu, Fengpeng, Wu, Bingyue, Zhang, Xiaoxiao, Yang, Congrong, Zhou, Chaoxi, Ren, Shuguang, Wang, Jun, Yang, Yafan, Wang, Guiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386172/
https://www.ncbi.nlm.nih.gov/pubmed/34458143
http://dx.doi.org/10.3389/fonc.2021.699475
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author Wu, Fengpeng
Wu, Bingyue
Zhang, Xiaoxiao
Yang, Congrong
Zhou, Chaoxi
Ren, Shuguang
Wang, Jun
Yang, Yafan
Wang, Guiying
author_facet Wu, Fengpeng
Wu, Bingyue
Zhang, Xiaoxiao
Yang, Congrong
Zhou, Chaoxi
Ren, Shuguang
Wang, Jun
Yang, Yafan
Wang, Guiying
author_sort Wu, Fengpeng
collection PubMed
description Neoadjuvant chemoradiotherapy has been widely used in the treatment of locally advanced rectal cancer due to the excellent advantages of irradiation in cancer therapy. Unfortunately, not every patient can benefit from this treatment, therefore, it is of great significance to explore biomarkers that can predict irradiation sensitivity. In this study, we screened microRNAs (miRNAs) which were positively correlated with irradiation resistance and found that miRNA-552 and miRNA-183 families were positively correlated with the irradiation resistance of rectal cancer, and found that high expression of miRNA-96-5p enhanced the irradiation resistance of rectal cancer cells through direct regulation of the GPC3 gene and abnormal activation of the canonical Wnt signal transduction pathway. Based on the radioreactivity results of patient-derived xenograft models, this is the first screening report for radio-resistant biomarkers in rectal cancer. Our results suggest that miRNA-96-5p expression is an important factor affecting the radiation response of colorectal cancer cells.
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spelling pubmed-83861722021-08-26 Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells Wu, Fengpeng Wu, Bingyue Zhang, Xiaoxiao Yang, Congrong Zhou, Chaoxi Ren, Shuguang Wang, Jun Yang, Yafan Wang, Guiying Front Oncol Oncology Neoadjuvant chemoradiotherapy has been widely used in the treatment of locally advanced rectal cancer due to the excellent advantages of irradiation in cancer therapy. Unfortunately, not every patient can benefit from this treatment, therefore, it is of great significance to explore biomarkers that can predict irradiation sensitivity. In this study, we screened microRNAs (miRNAs) which were positively correlated with irradiation resistance and found that miRNA-552 and miRNA-183 families were positively correlated with the irradiation resistance of rectal cancer, and found that high expression of miRNA-96-5p enhanced the irradiation resistance of rectal cancer cells through direct regulation of the GPC3 gene and abnormal activation of the canonical Wnt signal transduction pathway. Based on the radioreactivity results of patient-derived xenograft models, this is the first screening report for radio-resistant biomarkers in rectal cancer. Our results suggest that miRNA-96-5p expression is an important factor affecting the radiation response of colorectal cancer cells. Frontiers Media S.A. 2021-08-11 /pmc/articles/PMC8386172/ /pubmed/34458143 http://dx.doi.org/10.3389/fonc.2021.699475 Text en Copyright © 2021 Wu, Wu, Zhang, Yang, Zhou, Ren, Wang, Yang and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Wu, Fengpeng
Wu, Bingyue
Zhang, Xiaoxiao
Yang, Congrong
Zhou, Chaoxi
Ren, Shuguang
Wang, Jun
Yang, Yafan
Wang, Guiying
Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells
title Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells
title_full Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells
title_fullStr Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells
title_full_unstemmed Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells
title_short Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells
title_sort screening of microrna related to irradiation response and the regulation mechanism of mirna-96-5p in rectal cancer cells
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386172/
https://www.ncbi.nlm.nih.gov/pubmed/34458143
http://dx.doi.org/10.3389/fonc.2021.699475
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