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Evaluation of the cargo contents and potential role of extracellular vesicles in osteoporosis

Osteoporosis is a common aging-related disease diagnosed primarily using bone mineral density (BMD). Extracellular vesicles (EVs) remain unexplored in the context of osteoporosis. Towards this, EVs were isolated from plasma of a discovery cohort with 8 non-osteoporotic and 8 osteoporotic individuals...

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Autores principales: Xu, Jifeng, Chen, Yu, Yu, Dongsheng, Zhang, Li, Dou, Xiaofan, Wu, Gang, Wang, Yaping, Zhang, Shuijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386556/
https://www.ncbi.nlm.nih.gov/pubmed/34375951
http://dx.doi.org/10.18632/aging.203264
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author Xu, Jifeng
Chen, Yu
Yu, Dongsheng
Zhang, Li
Dou, Xiaofan
Wu, Gang
Wang, Yaping
Zhang, Shuijun
author_facet Xu, Jifeng
Chen, Yu
Yu, Dongsheng
Zhang, Li
Dou, Xiaofan
Wu, Gang
Wang, Yaping
Zhang, Shuijun
author_sort Xu, Jifeng
collection PubMed
description Osteoporosis is a common aging-related disease diagnosed primarily using bone mineral density (BMD). Extracellular vesicles (EVs) remain unexplored in the context of osteoporosis. Towards this, EVs were isolated from plasma of a discovery cohort with 8 non-osteoporotic and 8 osteoporotic individuals, and nanoparticle tracking analysis (NTA) revealed a significantly higher EV concentration in osteoporotic individuals (P = 0.003). Moreover, EVs concentration showed a linear correlation with bone mineral density (BMD) values (linear correlation coefficient r = 0.9542, deviation from zero, p < 0.001). Results using a mouse model of osteoporosis confirmed that the number of EVs in mice from hindlimb unloading group was significantly higher than that from the age-matched control group (p = 0.015). TaqMan Real-Time PCR demonstrated that miR-335-5p, -320a, -483-5p, and miR-21-5p, were significantly higher expressed in osteoporotic patients compared with non-osteoporotic individuals. Quantitative real-time PCR shown that Wnt1, Wnt5a, Wnt7a, and Wnt9a mRNAs were lower expressed in osteoporosis derived EVs. In vitro functional assay indicated that osteoporosis derived EVs resulted in reduced mineralization in SaOS-2 cells. In conclusion, these results suggest that osteoporosis increased the secretion of EVs which carry higher expression of miRNAs and decreased expression of Wnt signals, further decreased the mineralization capacity in human osteoblasts.
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spelling pubmed-83865562021-08-27 Evaluation of the cargo contents and potential role of extracellular vesicles in osteoporosis Xu, Jifeng Chen, Yu Yu, Dongsheng Zhang, Li Dou, Xiaofan Wu, Gang Wang, Yaping Zhang, Shuijun Aging (Albany NY) Research Paper Osteoporosis is a common aging-related disease diagnosed primarily using bone mineral density (BMD). Extracellular vesicles (EVs) remain unexplored in the context of osteoporosis. Towards this, EVs were isolated from plasma of a discovery cohort with 8 non-osteoporotic and 8 osteoporotic individuals, and nanoparticle tracking analysis (NTA) revealed a significantly higher EV concentration in osteoporotic individuals (P = 0.003). Moreover, EVs concentration showed a linear correlation with bone mineral density (BMD) values (linear correlation coefficient r = 0.9542, deviation from zero, p < 0.001). Results using a mouse model of osteoporosis confirmed that the number of EVs in mice from hindlimb unloading group was significantly higher than that from the age-matched control group (p = 0.015). TaqMan Real-Time PCR demonstrated that miR-335-5p, -320a, -483-5p, and miR-21-5p, were significantly higher expressed in osteoporotic patients compared with non-osteoporotic individuals. Quantitative real-time PCR shown that Wnt1, Wnt5a, Wnt7a, and Wnt9a mRNAs were lower expressed in osteoporosis derived EVs. In vitro functional assay indicated that osteoporosis derived EVs resulted in reduced mineralization in SaOS-2 cells. In conclusion, these results suggest that osteoporosis increased the secretion of EVs which carry higher expression of miRNAs and decreased expression of Wnt signals, further decreased the mineralization capacity in human osteoblasts. Impact Journals 2021-08-10 /pmc/articles/PMC8386556/ /pubmed/34375951 http://dx.doi.org/10.18632/aging.203264 Text en Copyright: © 2021 Xu et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Xu, Jifeng
Chen, Yu
Yu, Dongsheng
Zhang, Li
Dou, Xiaofan
Wu, Gang
Wang, Yaping
Zhang, Shuijun
Evaluation of the cargo contents and potential role of extracellular vesicles in osteoporosis
title Evaluation of the cargo contents and potential role of extracellular vesicles in osteoporosis
title_full Evaluation of the cargo contents and potential role of extracellular vesicles in osteoporosis
title_fullStr Evaluation of the cargo contents and potential role of extracellular vesicles in osteoporosis
title_full_unstemmed Evaluation of the cargo contents and potential role of extracellular vesicles in osteoporosis
title_short Evaluation of the cargo contents and potential role of extracellular vesicles in osteoporosis
title_sort evaluation of the cargo contents and potential role of extracellular vesicles in osteoporosis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386556/
https://www.ncbi.nlm.nih.gov/pubmed/34375951
http://dx.doi.org/10.18632/aging.203264
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