Benchmarking laboratory processes to characterise low-biomass respiratory microbiota

The low biomass of respiratory samples makes it difficult to accurately characterise the microbial community composition. PCR conditions and contaminating microbial DNA can alter the biological profile. The objective of this study was to benchmark the currently available laboratory protocols to accu...

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Detalles Bibliográficos
Autores principales: Hasrat, Raiza, Kool, Jolanda, de Steenhuijsen Piters, Wouter A. A., Chu, Mei Ling J. N., Kuiling, Sjoerd, Groot, James A., van Logchem, Elske M., Fuentes, Susana, Franz, Eelco, Bogaert, Debby, Bosch, Thijs
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387476/
https://www.ncbi.nlm.nih.gov/pubmed/34433845
http://dx.doi.org/10.1038/s41598-021-96556-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387476/
https://www.ncbi.nlm.nih.gov/pubmed/34433845
http://dx.doi.org/10.1038/s41598-021-96556-5

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