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Benchmarking laboratory processes to characterise low-biomass respiratory microbiota
The low biomass of respiratory samples makes it difficult to accurately characterise the microbial community composition. PCR conditions and contaminating microbial DNA can alter the biological profile. The objective of this study was to benchmark the currently available laboratory protocols to accu...
Autores principales: | Hasrat, Raiza, Kool, Jolanda, de Steenhuijsen Piters, Wouter A. A., Chu, Mei Ling J. N., Kuiling, Sjoerd, Groot, James A., van Logchem, Elske M., Fuentes, Susana, Franz, Eelco, Bogaert, Debby, Bosch, Thijs |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387476/ https://www.ncbi.nlm.nih.gov/pubmed/34433845 http://dx.doi.org/10.1038/s41598-021-96556-5 |
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