Protocol for using fluorescent sensors targeted to endogenous proteins (FluoSTEPs) to measure microdomain-specific signaling events

Fluorescence-based sensors are powerful molecular tools for studying the spatiotemporal regulation of cell signaling, which is often organized into discrete microdomains. Here, we present a protocol for using fluorescent sensors targeted to endogenous proteins (FluoSTEPs), a new class of fluorescent...

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Detalles Bibliográficos
Autores principales: Zhang, Jason Z., Tenner, Brian, Mehta, Sohum, Zhang, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387570/
https://www.ncbi.nlm.nih.gov/pubmed/34467224
http://dx.doi.org/10.1016/j.xpro.2021.100693

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387570/
https://www.ncbi.nlm.nih.gov/pubmed/34467224
http://dx.doi.org/10.1016/j.xpro.2021.100693

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