Treadmill-based task for assessing spatial memory in head-fixed mice

Several mouse in vivo neuronal recording techniques require head fixation. Head-fixed treadmill walking can be used to design tasks that enable the study of neural activity in the context of behavior. Here, we provide a detailed protocol for constructing a treadmill with tactile spatial cues, traini...

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Detalles Bibliográficos
Autores principales: Jordan, Jake T., McDermott, Kelsey D., Frechou, M. Agustina, Shtrahman, Matthew, Gonçalves, J. Tiago
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387801/
https://www.ncbi.nlm.nih.gov/pubmed/34471907
http://dx.doi.org/10.1016/j.xpro.2021.100770
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author Jordan, Jake T.
McDermott, Kelsey D.
Frechou, M. Agustina
Shtrahman, Matthew
Gonçalves, J. Tiago
author_facet Jordan, Jake T.
McDermott, Kelsey D.
Frechou, M. Agustina
Shtrahman, Matthew
Gonçalves, J. Tiago
author_sort Jordan, Jake T.
collection PubMed
description Several mouse in vivo neuronal recording techniques require head fixation. Head-fixed treadmill walking can be used to design tasks that enable the study of neural activity in the context of behavior. Here, we provide a detailed protocol for constructing a treadmill with tactile spatial cues, training mice on a rewarded behavioral task, and analyzing behavioral data. We discuss common problems and solutions we have developed to optimize training. Finally, we demonstrate how to test spatial memory performance using this task.
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spelling pubmed-83878012021-08-31 Treadmill-based task for assessing spatial memory in head-fixed mice Jordan, Jake T. McDermott, Kelsey D. Frechou, M. Agustina Shtrahman, Matthew Gonçalves, J. Tiago STAR Protoc Protocol Several mouse in vivo neuronal recording techniques require head fixation. Head-fixed treadmill walking can be used to design tasks that enable the study of neural activity in the context of behavior. Here, we provide a detailed protocol for constructing a treadmill with tactile spatial cues, training mice on a rewarded behavioral task, and analyzing behavioral data. We discuss common problems and solutions we have developed to optimize training. Finally, we demonstrate how to test spatial memory performance using this task. Elsevier 2021-08-21 /pmc/articles/PMC8387801/ /pubmed/34471907 http://dx.doi.org/10.1016/j.xpro.2021.100770 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Jordan, Jake T.
McDermott, Kelsey D.
Frechou, M. Agustina
Shtrahman, Matthew
Gonçalves, J. Tiago
Treadmill-based task for assessing spatial memory in head-fixed mice
title Treadmill-based task for assessing spatial memory in head-fixed mice
title_full Treadmill-based task for assessing spatial memory in head-fixed mice
title_fullStr Treadmill-based task for assessing spatial memory in head-fixed mice
title_full_unstemmed Treadmill-based task for assessing spatial memory in head-fixed mice
title_short Treadmill-based task for assessing spatial memory in head-fixed mice
title_sort treadmill-based task for assessing spatial memory in head-fixed mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387801/
https://www.ncbi.nlm.nih.gov/pubmed/34471907
http://dx.doi.org/10.1016/j.xpro.2021.100770
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