Common occurrence of Enterocytozoon bieneusi genotypes SHR1 and PL2 in farmed masked palm civet (Paguma larvata) in China

Enterocytozoon bieneusi is a common pathogen in a broad range of vertebrate hosts. To assess the prevalence and genotypes of E. bieneusi in farmed masked palm civet (Paguma larvata), 537 fecal samples from seven provinces in China were tested by nested PCR of the polymorphic internal transcribed spa...

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Autores principales: Lin, Xuhui, Xin, Luyao, Cao, Yangwenna, Hou, Minyu, Qiao, Fei, Li, Juan, Qi, Meng, Sun, Mingfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387836/
https://www.ncbi.nlm.nih.gov/pubmed/34471599
http://dx.doi.org/10.1016/j.ijppaw.2021.08.009
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author Lin, Xuhui
Xin, Luyao
Cao, Yangwenna
Hou, Minyu
Qiao, Fei
Li, Juan
Qi, Meng
Sun, Mingfei
author_facet Lin, Xuhui
Xin, Luyao
Cao, Yangwenna
Hou, Minyu
Qiao, Fei
Li, Juan
Qi, Meng
Sun, Mingfei
author_sort Lin, Xuhui
collection PubMed
description Enterocytozoon bieneusi is a common pathogen in a broad range of vertebrate hosts. To assess the prevalence and genotypes of E. bieneusi in farmed masked palm civet (Paguma larvata), 537 fecal samples from seven provinces in China were tested by nested PCR of the polymorphic internal transcribed spacer (ITS) region. Among of all the samples, 60.0% (325/531) were positive for E. bieneusi, with the highest prevalence in Hebei province (85.0%). Sequence analysis revealed the presence of nine E. bieneusi genotypes, including four known genotypes (SHR1, PL2, PL4, CHG19) and five novel genotypes (PL12 to PL16). Genotypes SHR1 and PL2 were the most common genotypes in seven provinces. Phylogenetic analysis showed that three genotypes (CHG19, PL4 and PL16) were distributed to Group 1, and six genotypes (SHR1, PL2, PL12, PL13, PL14 and PL15) formed a novel clade, which was named group 12. Findings highlight the need to conduct additional research to elucidate the epidemiology of E. bieneusi in farmed masked palm civet.
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spelling pubmed-83878362021-08-31 Common occurrence of Enterocytozoon bieneusi genotypes SHR1 and PL2 in farmed masked palm civet (Paguma larvata) in China Lin, Xuhui Xin, Luyao Cao, Yangwenna Hou, Minyu Qiao, Fei Li, Juan Qi, Meng Sun, Mingfei Int J Parasitol Parasites Wildl Article Enterocytozoon bieneusi is a common pathogen in a broad range of vertebrate hosts. To assess the prevalence and genotypes of E. bieneusi in farmed masked palm civet (Paguma larvata), 537 fecal samples from seven provinces in China were tested by nested PCR of the polymorphic internal transcribed spacer (ITS) region. Among of all the samples, 60.0% (325/531) were positive for E. bieneusi, with the highest prevalence in Hebei province (85.0%). Sequence analysis revealed the presence of nine E. bieneusi genotypes, including four known genotypes (SHR1, PL2, PL4, CHG19) and five novel genotypes (PL12 to PL16). Genotypes SHR1 and PL2 were the most common genotypes in seven provinces. Phylogenetic analysis showed that three genotypes (CHG19, PL4 and PL16) were distributed to Group 1, and six genotypes (SHR1, PL2, PL12, PL13, PL14 and PL15) formed a novel clade, which was named group 12. Findings highlight the need to conduct additional research to elucidate the epidemiology of E. bieneusi in farmed masked palm civet. Elsevier 2021-08-21 /pmc/articles/PMC8387836/ /pubmed/34471599 http://dx.doi.org/10.1016/j.ijppaw.2021.08.009 Text en © 2021 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Lin, Xuhui
Xin, Luyao
Cao, Yangwenna
Hou, Minyu
Qiao, Fei
Li, Juan
Qi, Meng
Sun, Mingfei
Common occurrence of Enterocytozoon bieneusi genotypes SHR1 and PL2 in farmed masked palm civet (Paguma larvata) in China
title Common occurrence of Enterocytozoon bieneusi genotypes SHR1 and PL2 in farmed masked palm civet (Paguma larvata) in China
title_full Common occurrence of Enterocytozoon bieneusi genotypes SHR1 and PL2 in farmed masked palm civet (Paguma larvata) in China
title_fullStr Common occurrence of Enterocytozoon bieneusi genotypes SHR1 and PL2 in farmed masked palm civet (Paguma larvata) in China
title_full_unstemmed Common occurrence of Enterocytozoon bieneusi genotypes SHR1 and PL2 in farmed masked palm civet (Paguma larvata) in China
title_short Common occurrence of Enterocytozoon bieneusi genotypes SHR1 and PL2 in farmed masked palm civet (Paguma larvata) in China
title_sort common occurrence of enterocytozoon bieneusi genotypes shr1 and pl2 in farmed masked palm civet (paguma larvata) in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387836/
https://www.ncbi.nlm.nih.gov/pubmed/34471599
http://dx.doi.org/10.1016/j.ijppaw.2021.08.009
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