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Prednisone prevents particle induced bone loss in the calvaria mouse model

INTRODUCTION: Glucocorticoids are essential in the treatment of many chronic inflammatory and malignant diseases but are known to have detrimental effects on bone. This study aimed to investigate the effects of prednisone on osteoclast functioning in vivo in the calvaria particle-induced bone loss m...

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Autores principales: Schündeln, Michael M., Höppner, Jakob, Meyer, Felix L., Schmuck, Wiebke, Kauther, Max D., Hilken, Gero, Levkau, Bodo, Rauner, Martina, Grasemann, Corinna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387912/
https://www.ncbi.nlm.nih.gov/pubmed/34471710
http://dx.doi.org/10.1016/j.heliyon.2021.e07828
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author Schündeln, Michael M.
Höppner, Jakob
Meyer, Felix L.
Schmuck, Wiebke
Kauther, Max D.
Hilken, Gero
Levkau, Bodo
Rauner, Martina
Grasemann, Corinna
author_facet Schündeln, Michael M.
Höppner, Jakob
Meyer, Felix L.
Schmuck, Wiebke
Kauther, Max D.
Hilken, Gero
Levkau, Bodo
Rauner, Martina
Grasemann, Corinna
author_sort Schündeln, Michael M.
collection PubMed
description INTRODUCTION: Glucocorticoids are essential in the treatment of many chronic inflammatory and malignant diseases but are known to have detrimental effects on bone. This study aimed to investigate the effects of prednisone on osteoclast functioning in vivo in the calvaria particle-induced bone loss mouse model. METHODS: 12-week-old male C57BL6/J mice received subcutaneously implanted prednisone (2.5 mg/d, 60 day release (n = 14)) or placebo pellets (n = 10). Osteolysis of the calvaria bone was induced two weeks later by application of ultra-high-molecular-weight polyethylene- (UHMWPE) particles to the dome (vs sham operation). The extent of osteolysis was determined histologically and by micro-computer tomography. RESULTS: Prednisone significantly inhibited particle-induced osteolysis in the skull. No significant difference in osteoclast numbers was seen in mice with prednisone vs placebo treatment. Prednisone treatment alone without particle application did not reduce bone mineral density or deterioration in bone microarchitecture parameters. CONCLUSIONS: The calvaria particle-induced bone loss mouse model can be adapted to investigate osteoclast activity in vivo and the effect of prednisone on osteoclasts. In this preventive experimental design, the application of short-term low-dose prednisone has osteoprotective effects without measurable systemic side effects on bone parameters.
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spelling pubmed-83879122021-08-31 Prednisone prevents particle induced bone loss in the calvaria mouse model Schündeln, Michael M. Höppner, Jakob Meyer, Felix L. Schmuck, Wiebke Kauther, Max D. Hilken, Gero Levkau, Bodo Rauner, Martina Grasemann, Corinna Heliyon Research Article INTRODUCTION: Glucocorticoids are essential in the treatment of many chronic inflammatory and malignant diseases but are known to have detrimental effects on bone. This study aimed to investigate the effects of prednisone on osteoclast functioning in vivo in the calvaria particle-induced bone loss mouse model. METHODS: 12-week-old male C57BL6/J mice received subcutaneously implanted prednisone (2.5 mg/d, 60 day release (n = 14)) or placebo pellets (n = 10). Osteolysis of the calvaria bone was induced two weeks later by application of ultra-high-molecular-weight polyethylene- (UHMWPE) particles to the dome (vs sham operation). The extent of osteolysis was determined histologically and by micro-computer tomography. RESULTS: Prednisone significantly inhibited particle-induced osteolysis in the skull. No significant difference in osteoclast numbers was seen in mice with prednisone vs placebo treatment. Prednisone treatment alone without particle application did not reduce bone mineral density or deterioration in bone microarchitecture parameters. CONCLUSIONS: The calvaria particle-induced bone loss mouse model can be adapted to investigate osteoclast activity in vivo and the effect of prednisone on osteoclasts. In this preventive experimental design, the application of short-term low-dose prednisone has osteoprotective effects without measurable systemic side effects on bone parameters. Elsevier 2021-08-18 /pmc/articles/PMC8387912/ /pubmed/34471710 http://dx.doi.org/10.1016/j.heliyon.2021.e07828 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Schündeln, Michael M.
Höppner, Jakob
Meyer, Felix L.
Schmuck, Wiebke
Kauther, Max D.
Hilken, Gero
Levkau, Bodo
Rauner, Martina
Grasemann, Corinna
Prednisone prevents particle induced bone loss in the calvaria mouse model
title Prednisone prevents particle induced bone loss in the calvaria mouse model
title_full Prednisone prevents particle induced bone loss in the calvaria mouse model
title_fullStr Prednisone prevents particle induced bone loss in the calvaria mouse model
title_full_unstemmed Prednisone prevents particle induced bone loss in the calvaria mouse model
title_short Prednisone prevents particle induced bone loss in the calvaria mouse model
title_sort prednisone prevents particle induced bone loss in the calvaria mouse model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387912/
https://www.ncbi.nlm.nih.gov/pubmed/34471710
http://dx.doi.org/10.1016/j.heliyon.2021.e07828
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