Cargando…
Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus)
SIMPLE SUMMARY: In our study, different quantitative and qualitative molecular techniques were used to detect and genetically characterise Carnivore protoparvovirus 1, Canine adenovirus type 1 and 2 (CAdV-1 and CAdV-2), and Canine circovirus (CanineCV) in Italian wolves (Canis lupus italicus) of the...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388400/ https://www.ncbi.nlm.nih.gov/pubmed/34438655 http://dx.doi.org/10.3390/ani11082198 |
_version_ | 1783742633825271808 |
---|---|
author | Balboni, Andrea Urbani, Lorenza Delogu, Mauro Musto, Carmela Fontana, Maria Cristina Merialdi, Giuseppe Lucifora, Giuseppe Terrusi, Alessia Dondi, Francesco Battilani, Mara |
author_facet | Balboni, Andrea Urbani, Lorenza Delogu, Mauro Musto, Carmela Fontana, Maria Cristina Merialdi, Giuseppe Lucifora, Giuseppe Terrusi, Alessia Dondi, Francesco Battilani, Mara |
author_sort | Balboni, Andrea |
collection | PubMed |
description | SIMPLE SUMMARY: In our study, different quantitative and qualitative molecular techniques were used to detect and genetically characterise Carnivore protoparvovirus 1, Canine adenovirus type 1 and 2 (CAdV-1 and CAdV-2), and Canine circovirus (CanineCV) in Italian wolves (Canis lupus italicus) of the Italian Apennines. Carnivore protoparvoviruses were the most frequently detected viruses, followed by CanineCV and CAdV. All the wolves tested positive for at least one of the DNA viruses screened, and 47.8% of the subjects were coinfected with two or three viruses. From viral sequences analysis, close correlations emerged between the viruses identified in the wolves and those circulating in domestic dogs, suggesting that the same viruses infect wolves and domestic dogs. Further studies are needed to investigate if pathogens are transmitted between the two species. ABSTRACT: In this study, internal organs (tongue, intestine, and spleen) of 23 free-ranging Italian wolves (Canis lupus italicus) found dead between 2017 and 2019 were tested for Carnivore protoparvovirus 1, Canine adenovirus (CAdV), and Canine circovirus (CanineCV) using real-time PCR assays. Genetic characterisation of the identified viruses was carried out by amplification, sequencing, and analysis of the complete viral genome or informative viral genes. All the wolves tested positive for at least one of the DNA viruses screened, and 11/23 were coinfected. Carnivore protoparvoviruses were the most frequently detected viruses (21/23), followed by CanineCV (11/23) and CAdV (4/23). From the analysis of the partial VP2 gene of 13 carnivore protoparvoviruses, 12 were canine parvovirus type 2b, closely related to the strains detected in dogs and wild carnivores from Italy, and one was a feline panleukopenia-like virus. Of the four CAdV identified, two were CAdV-1 and two were CAdV-2. The complete genome of seven CanineCVs was sequenced and related to the CanineCV identified in dogs, wolves, and foxes worldwide. Close correlations emerged between the viruses identified in wolves and those circulating in domestic dogs. Further studies are needed to investigate if these pathogens may be potentially cross-transmitted between the two species. |
format | Online Article Text |
id | pubmed-8388400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83884002021-08-27 Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus) Balboni, Andrea Urbani, Lorenza Delogu, Mauro Musto, Carmela Fontana, Maria Cristina Merialdi, Giuseppe Lucifora, Giuseppe Terrusi, Alessia Dondi, Francesco Battilani, Mara Animals (Basel) Article SIMPLE SUMMARY: In our study, different quantitative and qualitative molecular techniques were used to detect and genetically characterise Carnivore protoparvovirus 1, Canine adenovirus type 1 and 2 (CAdV-1 and CAdV-2), and Canine circovirus (CanineCV) in Italian wolves (Canis lupus italicus) of the Italian Apennines. Carnivore protoparvoviruses were the most frequently detected viruses, followed by CanineCV and CAdV. All the wolves tested positive for at least one of the DNA viruses screened, and 47.8% of the subjects were coinfected with two or three viruses. From viral sequences analysis, close correlations emerged between the viruses identified in the wolves and those circulating in domestic dogs, suggesting that the same viruses infect wolves and domestic dogs. Further studies are needed to investigate if pathogens are transmitted between the two species. ABSTRACT: In this study, internal organs (tongue, intestine, and spleen) of 23 free-ranging Italian wolves (Canis lupus italicus) found dead between 2017 and 2019 were tested for Carnivore protoparvovirus 1, Canine adenovirus (CAdV), and Canine circovirus (CanineCV) using real-time PCR assays. Genetic characterisation of the identified viruses was carried out by amplification, sequencing, and analysis of the complete viral genome or informative viral genes. All the wolves tested positive for at least one of the DNA viruses screened, and 11/23 were coinfected. Carnivore protoparvoviruses were the most frequently detected viruses (21/23), followed by CanineCV (11/23) and CAdV (4/23). From the analysis of the partial VP2 gene of 13 carnivore protoparvoviruses, 12 were canine parvovirus type 2b, closely related to the strains detected in dogs and wild carnivores from Italy, and one was a feline panleukopenia-like virus. Of the four CAdV identified, two were CAdV-1 and two were CAdV-2. The complete genome of seven CanineCVs was sequenced and related to the CanineCV identified in dogs, wolves, and foxes worldwide. Close correlations emerged between the viruses identified in wolves and those circulating in domestic dogs. Further studies are needed to investigate if these pathogens may be potentially cross-transmitted between the two species. MDPI 2021-07-24 /pmc/articles/PMC8388400/ /pubmed/34438655 http://dx.doi.org/10.3390/ani11082198 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Balboni, Andrea Urbani, Lorenza Delogu, Mauro Musto, Carmela Fontana, Maria Cristina Merialdi, Giuseppe Lucifora, Giuseppe Terrusi, Alessia Dondi, Francesco Battilani, Mara Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus) |
title | Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus) |
title_full | Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus) |
title_fullStr | Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus) |
title_full_unstemmed | Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus) |
title_short | Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus) |
title_sort | integrated use of molecular techniques to detect and genetically characterise dna viruses in italian wolves (canis lupus italicus) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388400/ https://www.ncbi.nlm.nih.gov/pubmed/34438655 http://dx.doi.org/10.3390/ani11082198 |
work_keys_str_mv | AT balboniandrea integrateduseofmoleculartechniquestodetectandgeneticallycharacterisednavirusesinitalianwolvescanislupusitalicus AT urbanilorenza integrateduseofmoleculartechniquestodetectandgeneticallycharacterisednavirusesinitalianwolvescanislupusitalicus AT delogumauro integrateduseofmoleculartechniquestodetectandgeneticallycharacterisednavirusesinitalianwolvescanislupusitalicus AT mustocarmela integrateduseofmoleculartechniquestodetectandgeneticallycharacterisednavirusesinitalianwolvescanislupusitalicus AT fontanamariacristina integrateduseofmoleculartechniquestodetectandgeneticallycharacterisednavirusesinitalianwolvescanislupusitalicus AT merialdigiuseppe integrateduseofmoleculartechniquestodetectandgeneticallycharacterisednavirusesinitalianwolvescanislupusitalicus AT luciforagiuseppe integrateduseofmoleculartechniquestodetectandgeneticallycharacterisednavirusesinitalianwolvescanislupusitalicus AT terrusialessia integrateduseofmoleculartechniquestodetectandgeneticallycharacterisednavirusesinitalianwolvescanislupusitalicus AT dondifrancesco integrateduseofmoleculartechniquestodetectandgeneticallycharacterisednavirusesinitalianwolvescanislupusitalicus AT battilanimara integrateduseofmoleculartechniquestodetectandgeneticallycharacterisednavirusesinitalianwolvescanislupusitalicus |