Impact of Different Durations of Fasting on Intestinal Autophagy and Serum Metabolome in Broiler Chicken

SIMPLE SUMMARY: Fasting is usually used before metabolizable energy assessment in poultry. Recently, fasting-induced autophagy has been of concern because of the beneficial function of autophagy. In this study, we found that the intestinal autophagy gene Atg7 has a good quadratic fitting with fasting duration. We found that the serum metabolism pathways involved in glycerophospholipid, phenylalanine, GnRH signaling pathways, glycosylphosphatidylinositol anchor biosynthesis, autophagy, and ferroptosis changed with fasting. Furthermore, we found a correlation between intestinal autophagy and serum metabolite PE (18:3(9Z,12Z,15Z)/P-18:0). ABSTRACT: Fasting-induced autophagy in the intestine is beneficial for body health. This study was designed to explore the relationship between the host metabolism and intestinal autophagy. Broilers were randomly assigned into 48 cages. At 0 (CT), 12 (FH12), 24 (FH24), 36 (FH36), 48(FH48), and 72 h (FH72) before 09:00 a.m. on day 25, eight cages of birds were randomly allotted to each fasting time point using completely random design, and their food was removed. At 09:00 a.m. on day 25, the blood and jejunum were sampled for serum metabolome and autophagy gene analyses, respectively. The results showed that the autophagy gene Atg7 has a good quadratic fit with fasting duration (R(2) = 0.432, p < 0.001). Serum phosphatidylethanolamine (PE) and lyso-PE were decreased in the birds that were fasted for 24 h or longer. Conversely, the serum phosphatidylcholine (PC) and lyso-PC were increased in the birds that were fasted for 36 h or longer. Metabolism pathway analysis showed that the serum glycerophospholipid, phenylalanine, and GnRH signaling pathways were downregulated with the extended fasting duration. The serum metabolites involved in glycosylphosphatidylinositol anchor biosynthesis, autophagy, and ferroptosis were upregulated in all of the fasted groups. Correlation analysis showed that serum PE (18:3(9Z,12Z,15Z)/P-18:0) was a potential biomarker for intestinal autophagy. Our findings provide a potential biomarker related to intestinal autophagy.