Toxicity of Zinc Oxide Nanoparticles on the Embryo of Javanese Medaka (Oryzias javanicus Bleeker, 1854): A Comparative Study

SIMPLE SUMMARY: In recent years, the production and distribution of ZnO NPs have gradually increased. As the number of ZnO NPs containing products grows, and the release of these products into the environment—particularly to the aquatic environment—has increased, several questions about their toxic effects on aquatic organisms have arisen. In this study, we explore the embryotoxicity of ZnO NPs by using the newly introduced model organism Oryzias javanicus (Javanese medaka). We found that the 96 h LC(50) of ZnO NPs on the embryo of Javanese medaka were 0.643 mg/L, 1.333 mg/L, and 2.370 mg/L in ultra-pure, deionized, and dechlorinated tap water. The toxicity of ZnO NPs increased as both the concentration and time of exposure increased. The results of this study demonstrate that ZnO NPs are extremely toxic for the early life stage of Javanese medaka. ABSTRACT: (1) Background: Zinc oxide nanoparticles (ZnO NPs) are widely applied in various human products. However, they can be extremely toxic for aquatic organisms, particularly fish. This research was conducted to determine the LC(50) of ZnO NPs on the embryos of Javanese medaka (Oryzias javanicus) in ultra-pure, deionized, and dechlorinated tap water; (2) Methods: The experiments were conducted in a completely randomized design (CRD) with three replicates for six treatments for acute (0.100, 0.250, 0.500, 1.00, 5.00, and 10.00 mg/L) exposures for each type of water; (3) Results: The LC(50) of ZnO NPs at 96 h was determined as 0.643 mg/L in ultra-pure water, 1.333 mg/L in deionized water, and 2.370 in dechlorinated tap water. In addition to concentration-dependent toxicity, we also observed time-dependent toxicity for ZnO NPs. In addition, the sizes of ZnO NPs increased immediately after dispersion and were 1079 nm, 3209 nm, and 3652 nm in ultra-pure, deionized, and dechlorinated tap water. The highest concentration of measured Zn(2+) in exposure concentrations was found in ultra-pure water, followed by deionized and dechlorinated tap water suspensions. Furthermore, Javanese medaka showed high sensitivity to acute exposure of ZnO NPs in all types of water.