Bacterial Community Characteristics in the Gastrointestinal Tract of Yak (Bos grunniens) Fully Grazed on Pasture of the Qinghai-Tibetan Plateau of China

SIMPLE SUMMARY: The Qinghai–Tibetan plateau is considered as the third Pole of the world and is characterized by low oxygen, high altitude, extreme cold weather and strong ultraviolet radiation. Yak, as the main domestic animals raised on the plateau, play various roles in local herdsmen’s lives by supplying necessities such as meat, milk and fuel. Yak are adapted to the harsh environment on the plateau; microbiota in gut equip the hosts with special abilities including adaptability, as illustrated by numerous research projects. Accordingly, the microbes in the gastrointestinal tract of yak must be characteristically profiled as a strategy to adapt to the environment. However, little is known about the microbial community in whole tract of yak; almost all of reported researches focused on rumen. Therefore, in the current study the bacterial community in the gastrointestinal tract of yak was explored using 16S rDNA amplicon sequencing technology, and the community profiling characteristic in each section was clearly elucidated. ABSTRACT: In the current research, samples of yak gastrointestinal tracts (GITs) were used to profile the bacterial compositional characteristics using high through-put sequencing technology of 16S RNA amplicon. A total of 6959 OTUs was obtained from 20,799,614 effective tags, among which 751 OTUs were shared by ten sections. A total of 16 known phyla were obtained in all samples—the most abundant phyla were Firmicutes (34.58%), Bacteroidetes (33.96%) and Verrucomicrobia (11.70%). At the genus level, a total of 66 genera were obtained—Rikenellaceae_RC9_gut_group (7.24%), Akkermansia (6.32%) and Ruminococcaceae_UCG-005 (6.14%) were the most abundant. Species of Observed (Sob), Shannon and Chao values of the Stomach were the greatest, followed by the large intestine, while small intestine had the lowest diversity (p < 0.05). Bacteroidete were more abundant in sections from rumen to duodenum; while Firmicutes were the most abundant in sections from jejunum. ABC transporters (7.82%), Aminoacyl-tRNA biosynthesis (4.85%) and Purine metabolism (3.77%) were the most abundant level-3 pathways in all samples. The results of associated correlation analysis indicated that rectum samples might be used as an estimator of rumen bacterial communities and fermentation. The results of this research enrich the current knowledge about the unique animals of the QTP and extend our insight into GITs microecology of various animals.