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Levels of Main Bacterial Phyla in the Gastrointestinal Tract of Sheep Depending on Parity and Age
SIMPLE SUMMARY: The ruminant microbiome is considered a specific ecosystem found within the gastrointestinal tract. A balanced intestinal microbiota is important not only for maintaining gut homeostasis, but also for regulating immune function and has a direct impact on the gut–brain axis. Factors,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388517/ https://www.ncbi.nlm.nih.gov/pubmed/34438660 http://dx.doi.org/10.3390/ani11082203 |
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author | Smoliński, Jakub Szeligowska, Natalia Cholewińska, Paulina Czyż, Katarzyna Janczak, Marzena |
author_facet | Smoliński, Jakub Szeligowska, Natalia Cholewińska, Paulina Czyż, Katarzyna Janczak, Marzena |
author_sort | Smoliński, Jakub |
collection | PubMed |
description | SIMPLE SUMMARY: The ruminant microbiome is considered a specific ecosystem found within the gastrointestinal tract. A balanced intestinal microbiota is important not only for maintaining gut homeostasis, but also for regulating immune function and has a direct impact on the gut–brain axis. Factors, such as pregnancy, age, or genetics, can influence the microbial composition of the digestive system. The results of the study suggest that the parity, as well as the age of the animals, may affect the level of microorganisms in the digestive system. ABSTRACT: During pregnancy and parturition, the homeostasis of the body is disturbed, and the immune system is undermined, which is associated with hormonal changes within the body. Recently, it has also been suggested that physiological and hormonal changes associated with pregnancy may affect the composition of the gastrointestinal microbiome. Therefore, the aim of this study was to determine the composition of the microbiome in the third month of pregnancy in sheep in their first and second parity. Eighteen females in total were selected for the experiment, and they were divided into two groups: primiparous (aged 1 year) and multiparous ones (aged 2 years). The animals were fed the same fodder, and did not show any disease symptoms. Fecal samples were collected individually from each female (n = 20), and then bacterial DNA isolation and real-time PCR were performed for the main bacterial phyla (Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria) and families (Lactobacillaceae and Clostridia). The obtained results showed the differentiation in the microbiome between the primiparous and multiparous ewes with respect to the following groups: Bacteroidetes, Proteobacteria, and Actinobacteria—the level was higher in the case of the primiparas. These results suggest that the parity and age of the females may affect the gastrointestinal microbiome, but further studies are recommended. |
format | Online Article Text |
id | pubmed-8388517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83885172021-08-27 Levels of Main Bacterial Phyla in the Gastrointestinal Tract of Sheep Depending on Parity and Age Smoliński, Jakub Szeligowska, Natalia Cholewińska, Paulina Czyż, Katarzyna Janczak, Marzena Animals (Basel) Article SIMPLE SUMMARY: The ruminant microbiome is considered a specific ecosystem found within the gastrointestinal tract. A balanced intestinal microbiota is important not only for maintaining gut homeostasis, but also for regulating immune function and has a direct impact on the gut–brain axis. Factors, such as pregnancy, age, or genetics, can influence the microbial composition of the digestive system. The results of the study suggest that the parity, as well as the age of the animals, may affect the level of microorganisms in the digestive system. ABSTRACT: During pregnancy and parturition, the homeostasis of the body is disturbed, and the immune system is undermined, which is associated with hormonal changes within the body. Recently, it has also been suggested that physiological and hormonal changes associated with pregnancy may affect the composition of the gastrointestinal microbiome. Therefore, the aim of this study was to determine the composition of the microbiome in the third month of pregnancy in sheep in their first and second parity. Eighteen females in total were selected for the experiment, and they were divided into two groups: primiparous (aged 1 year) and multiparous ones (aged 2 years). The animals were fed the same fodder, and did not show any disease symptoms. Fecal samples were collected individually from each female (n = 20), and then bacterial DNA isolation and real-time PCR were performed for the main bacterial phyla (Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria) and families (Lactobacillaceae and Clostridia). The obtained results showed the differentiation in the microbiome between the primiparous and multiparous ewes with respect to the following groups: Bacteroidetes, Proteobacteria, and Actinobacteria—the level was higher in the case of the primiparas. These results suggest that the parity and age of the females may affect the gastrointestinal microbiome, but further studies are recommended. MDPI 2021-07-25 /pmc/articles/PMC8388517/ /pubmed/34438660 http://dx.doi.org/10.3390/ani11082203 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Smoliński, Jakub Szeligowska, Natalia Cholewińska, Paulina Czyż, Katarzyna Janczak, Marzena Levels of Main Bacterial Phyla in the Gastrointestinal Tract of Sheep Depending on Parity and Age |
title | Levels of Main Bacterial Phyla in the Gastrointestinal Tract of Sheep Depending on Parity and Age |
title_full | Levels of Main Bacterial Phyla in the Gastrointestinal Tract of Sheep Depending on Parity and Age |
title_fullStr | Levels of Main Bacterial Phyla in the Gastrointestinal Tract of Sheep Depending on Parity and Age |
title_full_unstemmed | Levels of Main Bacterial Phyla in the Gastrointestinal Tract of Sheep Depending on Parity and Age |
title_short | Levels of Main Bacterial Phyla in the Gastrointestinal Tract of Sheep Depending on Parity and Age |
title_sort | levels of main bacterial phyla in the gastrointestinal tract of sheep depending on parity and age |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388517/ https://www.ncbi.nlm.nih.gov/pubmed/34438660 http://dx.doi.org/10.3390/ani11082203 |
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