Pathological Manifestations of Francisella orientalis in the Green Texas Cichlid (Herichthys cyanoguttatus)

SIMPLE SUMMARY: The following study demonstrates the pathological manifestations of an emerging virulent bacterium, Francisella orientalis, in an ornamental cichlid fish, the green Texas cichlid (Herichthys cyanoguttatus). This study was conducted to prove that Francisella orientalis can cause a disease in the green Texas cichlid that is similar to natural infection. Francisella orientalis was discovered for the first time in green Texas cichlid by our team in Taiwan in 2015. The present study simply tried to prove the susceptibility of Francisella orientalis in green Texas cichlid by conducting a challenge experiment, where healthy fish were injected with a dose of the bacteria. After the challenge, the healthy fish showed the same disease progression as was seen in the case of natural outbreak. The mortality rate, clinical symptoms, gross findings, and histopathological findings were similar to natural infection. Francisella orientalis could also be recovered in artificial media from challenged fish, thus indicating that the bacteria had multiplied inside the fish. These findings prove that green Texas cichlid (Herichthys cyanoguttatus) is susceptible to Francisela orientalis, and new management and vaccination strategies are necessary in the farming of this fish. This study also helps by adding to the knowledge of the growing host base for Francisella orientalis. ABSTRACT: Francisella orientalis (Fo) is considered to be one of the major pathogens of tilapia because of the high mortalities observed during outbreaks. Other cichlids belonging to the same family (Cichlidae) as tilapia are also quite susceptible to this pathogen. On various occasions, Fo has also been isolated from other warm water fish, including three-line grunt, hybrid striped bass, French grunt, Caesar grunt, and Indo-Pacific reef fish. However, only a few studies have reported the pathogenicity of Francisella orientalis in ornamental cichlid fish. This study fulfills Koch’s postulates by showing that a strain of Fo obtained from green Texas cichlid (Herichthys cyanoguttatus) was able to produce the same pathogenicity in healthy fish. A mortality of 100% was observed after healthy green Texas cichlid were experimentally injected with Fo at a dose of 8.95 × 10(5) CFU/fish. DNA extracted from the organs of predilection (spleen, head kidney) gave positive results by PCR for all fish that died during the experimental period. Spleen and head kidney presented with multifocal white nodules in the affected fish, corresponding to typical vacuolated granulomas on histopathological examination of the tissues. Based on the results of this study, it is evident that Fo can indeed infect green Texas cichlid and produce a disease typical of francisellosis.