Characterisation of the Behavioural Effects of a Thoracic Squeeze in Healthy Newborn Piglets

SIMPLE SUMMARY: Firmly squeezing the chests of newborn foals and calves that are showing abnormal behaviours after birth causes them to enter a less-responsive state, characterised by lying down with eyes closed and no limb movements. Once the squeeze is removed, the newborns immediately ‘wake up’ and begin to display more normal behaviours. This response to the thoracic squeeze has also been observed in healthy, normally behaving foals. However, no studies have looked at the effects of the thoracic squeeze in healthy newborns of other mammalian species. We aimed to characterise the behavioural responses of healthy newborn piglets to a thoracic squeeze using the following two methods: a soft fabric rope, or a purpose-made inflation cuff. Behavioural data indicated that all piglets initially became less responsive, with reduced or absent reflex responses to a toe pinch or touch of the eyelid observed in over half of the piglets. The piglets squeezed with the inflation cuff appeared to enter a less-responsive state faster than the piglets squeezed with the rope. These findings suggest that the piglets responded to the thoracic squeeze in a similar way to healthy foals and that this may be a response conserved across multiple precocial mammalian species. Furthermore, the squeeze was found to be safe for inducing a less-responsive state in healthy piglets. This study provides a foundation for exploring the mechanisms underlying the responses to the thoracic squeeze and potential applications whilst performing husbandry procedures. ABSTRACT: A thoracic squeeze has been observed to cause both healthy and low vigour neonatal foals to enter a ‘less-responsive state’, characterised by loss of posture, eye closure and cessation of movement, from which they rapidly recover to express normal healthy behaviours when the squeeze is released. To date, there have been no systematic studies characterising the responses of healthy neonates of other mammalian species to a thoracic squeeze. We describe the responses of healthy newborn piglets (n = 17) to a standardised application of the thoracic squeeze and evaluate the effect of the method of squeeze application on the response. Neonatal piglets were squeezed around the chest with either a soft fabric rope as has been used in foals (n = 8) or a novel purpose-made inflation cuff (n = 9). Both methods were effective at inducing a less-responsive behavioural state in all piglets, with neural reflexes reduced or absent in over half of them. The inflation cuff appeared to induce the less-responsive state faster than the rope, and more piglets squeezed with the cuff remained in this state for the full 10-min squeeze. These findings suggest that the behavioural response of foals to thoracic squeezing can be generalised to neonates of other precocial mammalian species. This initial study provides a foundation for further research using the inflation cuff to explore mechanisms underlying the thoracic squeeze and ways in which it may be applied whilst performing husbandry procedures.