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Broth Microdilution and Gradient Diffusion Strips vs. Reference Agar Dilution Method: First Evaluation for Clostridiales Species Antimicrobial Susceptibility Testing

Antimicrobial susceptibility testing of anaerobes is challenging. Because MIC determination is recommended by both CLSI and EUCAST, commercial broth microdilution and diffusion strip tests have been developed. The reliability of broth microdilution methods has not been assessed yet using the agar di...

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Autores principales: Baquer, Florian, Ali Sawan, Asma, Auzou, Michel, Grillon, Antoine, Jaulhac, Benoît, Join-Lambert, Olivier, Boyer, Pierre H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388896/
https://www.ncbi.nlm.nih.gov/pubmed/34439025
http://dx.doi.org/10.3390/antibiotics10080975
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author Baquer, Florian
Ali Sawan, Asma
Auzou, Michel
Grillon, Antoine
Jaulhac, Benoît
Join-Lambert, Olivier
Boyer, Pierre H.
author_facet Baquer, Florian
Ali Sawan, Asma
Auzou, Michel
Grillon, Antoine
Jaulhac, Benoît
Join-Lambert, Olivier
Boyer, Pierre H.
author_sort Baquer, Florian
collection PubMed
description Antimicrobial susceptibility testing of anaerobes is challenging. Because MIC determination is recommended by both CLSI and EUCAST, commercial broth microdilution and diffusion strip tests have been developed. The reliability of broth microdilution methods has not been assessed yet using the agar dilution reference method. In this work, we evaluated two broth microdilution kits (MICRONAUT-S Anaerobes(®) MIC and Sensititre Anaerobe MIC(®)) and one gradient diffusion strip method (Liofilchem(®)) for antimicrobial susceptibility testing of 47 Clostridiales isolates (Clostridium, Clostridioides and Hungatella species) using the agar dilution method as a reference. The evaluation focused on comparing six antimicrobial molecules available in both microdilution kits. Analytical performances were evaluated according to the Food and Drug Administration (FDA) recommendations. Essential agreements (EA) and categorical agreements (CA) varied greatly according to the molecule and the evaluated method. Vancomycin had values of essential and categorical agreements above 90% for the three methods. The CA fulfilled the FDA criteria for three major molecules in the treatment of Gram-positive anaerobic infections (metronidazole, piperacillin/tazobactam and vancomycin). The highest rate of error was observed for clindamycin. Multicenter studies are needed to further validate these results.
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spelling pubmed-83888962021-08-27 Broth Microdilution and Gradient Diffusion Strips vs. Reference Agar Dilution Method: First Evaluation for Clostridiales Species Antimicrobial Susceptibility Testing Baquer, Florian Ali Sawan, Asma Auzou, Michel Grillon, Antoine Jaulhac, Benoît Join-Lambert, Olivier Boyer, Pierre H. Antibiotics (Basel) Article Antimicrobial susceptibility testing of anaerobes is challenging. Because MIC determination is recommended by both CLSI and EUCAST, commercial broth microdilution and diffusion strip tests have been developed. The reliability of broth microdilution methods has not been assessed yet using the agar dilution reference method. In this work, we evaluated two broth microdilution kits (MICRONAUT-S Anaerobes(®) MIC and Sensititre Anaerobe MIC(®)) and one gradient diffusion strip method (Liofilchem(®)) for antimicrobial susceptibility testing of 47 Clostridiales isolates (Clostridium, Clostridioides and Hungatella species) using the agar dilution method as a reference. The evaluation focused on comparing six antimicrobial molecules available in both microdilution kits. Analytical performances were evaluated according to the Food and Drug Administration (FDA) recommendations. Essential agreements (EA) and categorical agreements (CA) varied greatly according to the molecule and the evaluated method. Vancomycin had values of essential and categorical agreements above 90% for the three methods. The CA fulfilled the FDA criteria for three major molecules in the treatment of Gram-positive anaerobic infections (metronidazole, piperacillin/tazobactam and vancomycin). The highest rate of error was observed for clindamycin. Multicenter studies are needed to further validate these results. MDPI 2021-08-12 /pmc/articles/PMC8388896/ /pubmed/34439025 http://dx.doi.org/10.3390/antibiotics10080975 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baquer, Florian
Ali Sawan, Asma
Auzou, Michel
Grillon, Antoine
Jaulhac, Benoît
Join-Lambert, Olivier
Boyer, Pierre H.
Broth Microdilution and Gradient Diffusion Strips vs. Reference Agar Dilution Method: First Evaluation for Clostridiales Species Antimicrobial Susceptibility Testing
title Broth Microdilution and Gradient Diffusion Strips vs. Reference Agar Dilution Method: First Evaluation for Clostridiales Species Antimicrobial Susceptibility Testing
title_full Broth Microdilution and Gradient Diffusion Strips vs. Reference Agar Dilution Method: First Evaluation for Clostridiales Species Antimicrobial Susceptibility Testing
title_fullStr Broth Microdilution and Gradient Diffusion Strips vs. Reference Agar Dilution Method: First Evaluation for Clostridiales Species Antimicrobial Susceptibility Testing
title_full_unstemmed Broth Microdilution and Gradient Diffusion Strips vs. Reference Agar Dilution Method: First Evaluation for Clostridiales Species Antimicrobial Susceptibility Testing
title_short Broth Microdilution and Gradient Diffusion Strips vs. Reference Agar Dilution Method: First Evaluation for Clostridiales Species Antimicrobial Susceptibility Testing
title_sort broth microdilution and gradient diffusion strips vs. reference agar dilution method: first evaluation for clostridiales species antimicrobial susceptibility testing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388896/
https://www.ncbi.nlm.nih.gov/pubmed/34439025
http://dx.doi.org/10.3390/antibiotics10080975
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