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Whole-Genome Sequencing for Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Patients

Antibiotic resistance is a growing public health problem globally, incurring health and cost burdens. The occurrence of antibiotic-resistant bacterial infections has increased significantly over the years. Gram-negative bacteria display the broadest resistance range, with bacterial species expressin...

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Autores principales: Al Mana, Hassan, Sundararaju, Sathyavathi, Tsui, Clement K. M., Perez-Lopez, Andres, Yassine, Hadi, Al Thani, Asmaa, Al-Ansari, Khalid, Eltai, Nahla O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388976/
https://www.ncbi.nlm.nih.gov/pubmed/34439022
http://dx.doi.org/10.3390/antibiotics10080972
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author Al Mana, Hassan
Sundararaju, Sathyavathi
Tsui, Clement K. M.
Perez-Lopez, Andres
Yassine, Hadi
Al Thani, Asmaa
Al-Ansari, Khalid
Eltai, Nahla O.
author_facet Al Mana, Hassan
Sundararaju, Sathyavathi
Tsui, Clement K. M.
Perez-Lopez, Andres
Yassine, Hadi
Al Thani, Asmaa
Al-Ansari, Khalid
Eltai, Nahla O.
author_sort Al Mana, Hassan
collection PubMed
description Antibiotic resistance is a growing public health problem globally, incurring health and cost burdens. The occurrence of antibiotic-resistant bacterial infections has increased significantly over the years. Gram-negative bacteria display the broadest resistance range, with bacterial species expressing extended-spectrum β-lactamases (ESBLs), AmpC, and carbapenemases. All carbapenem-resistant Enterobacteriaceae (CRE) isolates from pediatric urinary tract infections (UTIs) between October 2015 and November 2019 (n = 30). All isolates underwent antimicrobial resistance phenotypic testing using the Phoenix NMIC/ID-5 panel, and carbapenemase production was confirmed using the NG-Test CARBA 5 assay. Whole-genome sequencing was performed on the CREs. The sequence type was identified using the Achtman multi-locus sequence typing scheme, and antimicrobial resistance markers were identified using ResFinder and the CARD database. The most common pathogens causing CRE UTIs were E. coli (63.3%) and K. pneumoniae (30%). The most common carbapenemases produced were OXA-48-like enzymes (46.6%) and NDM enzymes (40%). Additionally, one E. coli harbored IMP-26, and two K. pneumoniae possessed mutations in ompK37 and/or ompK36. Lastly, one E. coli had a mutation in the marA porin and efflux pump regulator. The findings highlight the difference in CRE epidemiology in the pediatric population compared to Qatar’s adult population, where NDM carbapenemases are more common.
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spelling pubmed-83889762021-08-27 Whole-Genome Sequencing for Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Patients Al Mana, Hassan Sundararaju, Sathyavathi Tsui, Clement K. M. Perez-Lopez, Andres Yassine, Hadi Al Thani, Asmaa Al-Ansari, Khalid Eltai, Nahla O. Antibiotics (Basel) Article Antibiotic resistance is a growing public health problem globally, incurring health and cost burdens. The occurrence of antibiotic-resistant bacterial infections has increased significantly over the years. Gram-negative bacteria display the broadest resistance range, with bacterial species expressing extended-spectrum β-lactamases (ESBLs), AmpC, and carbapenemases. All carbapenem-resistant Enterobacteriaceae (CRE) isolates from pediatric urinary tract infections (UTIs) between October 2015 and November 2019 (n = 30). All isolates underwent antimicrobial resistance phenotypic testing using the Phoenix NMIC/ID-5 panel, and carbapenemase production was confirmed using the NG-Test CARBA 5 assay. Whole-genome sequencing was performed on the CREs. The sequence type was identified using the Achtman multi-locus sequence typing scheme, and antimicrobial resistance markers were identified using ResFinder and the CARD database. The most common pathogens causing CRE UTIs were E. coli (63.3%) and K. pneumoniae (30%). The most common carbapenemases produced were OXA-48-like enzymes (46.6%) and NDM enzymes (40%). Additionally, one E. coli harbored IMP-26, and two K. pneumoniae possessed mutations in ompK37 and/or ompK36. Lastly, one E. coli had a mutation in the marA porin and efflux pump regulator. The findings highlight the difference in CRE epidemiology in the pediatric population compared to Qatar’s adult population, where NDM carbapenemases are more common. MDPI 2021-08-12 /pmc/articles/PMC8388976/ /pubmed/34439022 http://dx.doi.org/10.3390/antibiotics10080972 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al Mana, Hassan
Sundararaju, Sathyavathi
Tsui, Clement K. M.
Perez-Lopez, Andres
Yassine, Hadi
Al Thani, Asmaa
Al-Ansari, Khalid
Eltai, Nahla O.
Whole-Genome Sequencing for Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Patients
title Whole-Genome Sequencing for Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Patients
title_full Whole-Genome Sequencing for Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Patients
title_fullStr Whole-Genome Sequencing for Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Patients
title_full_unstemmed Whole-Genome Sequencing for Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Patients
title_short Whole-Genome Sequencing for Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Patients
title_sort whole-genome sequencing for molecular characterization of carbapenem-resistant enterobacteriaceae causing lower urinary tract infection among pediatric patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388976/
https://www.ncbi.nlm.nih.gov/pubmed/34439022
http://dx.doi.org/10.3390/antibiotics10080972
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