Cargando…
Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms
Bacterial biofilm infections are a major liability of medical implants, due to their resistance to both antibiotics and host immune response. Thermal shock can kill established biofilms, and some evidence suggests antibiotics may enhance this efficacy, despite having an insufficient effect themselve...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388990/ https://www.ncbi.nlm.nih.gov/pubmed/34439066 http://dx.doi.org/10.3390/antibiotics10081017 |
_version_ | 1783742759942750208 |
---|---|
author | Aljaafari, Haydar Gu, Yuejia Chicchelly, Hannah Nuxoll, Eric |
author_facet | Aljaafari, Haydar Gu, Yuejia Chicchelly, Hannah Nuxoll, Eric |
author_sort | Aljaafari, Haydar |
collection | PubMed |
description | Bacterial biofilm infections are a major liability of medical implants, due to their resistance to both antibiotics and host immune response. Thermal shock can kill established biofilms, and some evidence suggests antibiotics may enhance this efficacy, despite having an insufficient effect themselves. The nature of this interaction is unclear, however, complicating efforts to integrate thermal shock into implant infection treatment. This study aimed to determine whether these treatments were truly synergistic or simply orthogonal (i.e., independent). Pseudomonas aeruginosa biofilms of different architectures and stationary-phase population density were subjected to various thermal shocks, antibiotic exposures, or combinations thereof, and examined either immediately after treatment or after subsequent reincubation. Population decreases from the combination treatment matched the product of the decreases of individual treatments, indicating their orthogonality. However, reincubation showed binary behavior, where biofilms with an immediate population decrease beyond a critical factor (~10(4)) died off completely during reincubation, while biofilms with a smaller immediate decrease regrew. This critical factor was independent of the initial population density and the combination of treatments that achieved the immediate decrease. While antibiotics do not appear to enhance thermal shock directly, their contribution to achieving a critical population decrease for biofilm elimination can make the treatments appear strongly synergistic, strongly decreasing the intensity of thermal shock needed. |
format | Online Article Text |
id | pubmed-8388990 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83889902021-08-27 Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms Aljaafari, Haydar Gu, Yuejia Chicchelly, Hannah Nuxoll, Eric Antibiotics (Basel) Article Bacterial biofilm infections are a major liability of medical implants, due to their resistance to both antibiotics and host immune response. Thermal shock can kill established biofilms, and some evidence suggests antibiotics may enhance this efficacy, despite having an insufficient effect themselves. The nature of this interaction is unclear, however, complicating efforts to integrate thermal shock into implant infection treatment. This study aimed to determine whether these treatments were truly synergistic or simply orthogonal (i.e., independent). Pseudomonas aeruginosa biofilms of different architectures and stationary-phase population density were subjected to various thermal shocks, antibiotic exposures, or combinations thereof, and examined either immediately after treatment or after subsequent reincubation. Population decreases from the combination treatment matched the product of the decreases of individual treatments, indicating their orthogonality. However, reincubation showed binary behavior, where biofilms with an immediate population decrease beyond a critical factor (~10(4)) died off completely during reincubation, while biofilms with a smaller immediate decrease regrew. This critical factor was independent of the initial population density and the combination of treatments that achieved the immediate decrease. While antibiotics do not appear to enhance thermal shock directly, their contribution to achieving a critical population decrease for biofilm elimination can make the treatments appear strongly synergistic, strongly decreasing the intensity of thermal shock needed. MDPI 2021-08-21 /pmc/articles/PMC8388990/ /pubmed/34439066 http://dx.doi.org/10.3390/antibiotics10081017 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aljaafari, Haydar Gu, Yuejia Chicchelly, Hannah Nuxoll, Eric Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms |
title | Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms |
title_full | Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms |
title_fullStr | Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms |
title_full_unstemmed | Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms |
title_short | Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms |
title_sort | thermal shock and ciprofloxacin act orthogonally on pseudomonas aeruginosa biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388990/ https://www.ncbi.nlm.nih.gov/pubmed/34439066 http://dx.doi.org/10.3390/antibiotics10081017 |
work_keys_str_mv | AT aljaafarihaydar thermalshockandciprofloxacinactorthogonallyonpseudomonasaeruginosabiofilms AT guyuejia thermalshockandciprofloxacinactorthogonallyonpseudomonasaeruginosabiofilms AT chicchellyhannah thermalshockandciprofloxacinactorthogonallyonpseudomonasaeruginosabiofilms AT nuxolleric thermalshockandciprofloxacinactorthogonallyonpseudomonasaeruginosabiofilms |