Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability

Haloarchaea are extreme halophilic microorganisms belonging to the domain Archaea, phylum Euryarchaeota, and are producers of interesting antioxidant carotenoid compounds. In this study, four new strains of Haloarcula sp., isolated from saline lakes of the Atacama Desert, are reported and studied by...

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Autores principales: Lizama, Catherine, Romero-Parra, Javier, Andrade, Daniel, Riveros, Felipe, Bórquez, Jorge, Ahmed, Shakeel, Venegas-Salas, Luis, Cabalín, Carolina, Simirgiotis, Mario J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389013/
https://www.ncbi.nlm.nih.gov/pubmed/34439478
http://dx.doi.org/10.3390/antiox10081230
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author Lizama, Catherine
Romero-Parra, Javier
Andrade, Daniel
Riveros, Felipe
Bórquez, Jorge
Ahmed, Shakeel
Venegas-Salas, Luis
Cabalín, Carolina
Simirgiotis, Mario J.
author_facet Lizama, Catherine
Romero-Parra, Javier
Andrade, Daniel
Riveros, Felipe
Bórquez, Jorge
Ahmed, Shakeel
Venegas-Salas, Luis
Cabalín, Carolina
Simirgiotis, Mario J.
author_sort Lizama, Catherine
collection PubMed
description Haloarchaea are extreme halophilic microorganisms belonging to the domain Archaea, phylum Euryarchaeota, and are producers of interesting antioxidant carotenoid compounds. In this study, four new strains of Haloarcula sp., isolated from saline lakes of the Atacama Desert, are reported and studied by high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) for the first time. In addition, determination of the carotenoid pigment profile from the new strains of Haloarcula sp., plus two strains of Halorubrum tebenquichense, and their antioxidant activity by means of several methods is reported. The effect of biomass on cellular viability in skin cell lines was also evaluated by MTT assay. The cholinesterase inhibition capacity of six haloarchaea (Haloarcula sp. ALT-23; Haloarcula sp. TeSe-41; Haloarcula sp. TeSe-51; Haloarcula sp. Te Se-89 and Halorubrum tebenquichense strains TeSe-85 and Te Se-86) is also reported for the first time. AChE inhibition IC(50) was 2.96 ± 0.08 μg/mL and BuChE inhibition IC(50) was 2.39 ± 0.09 μg/mL for the most active strain, Halorubrum tebenquichense Te Se-85, respectively, which is more active in BuCHe than that of the standard galantamine. Docking calculation showed that carotenoids can exert their inhibitory activity fitting into the enzyme pocket by their halves, in the presence of cholinesterase dimers.
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spelling pubmed-83890132021-08-27 Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability Lizama, Catherine Romero-Parra, Javier Andrade, Daniel Riveros, Felipe Bórquez, Jorge Ahmed, Shakeel Venegas-Salas, Luis Cabalín, Carolina Simirgiotis, Mario J. Antioxidants (Basel) Article Haloarchaea are extreme halophilic microorganisms belonging to the domain Archaea, phylum Euryarchaeota, and are producers of interesting antioxidant carotenoid compounds. In this study, four new strains of Haloarcula sp., isolated from saline lakes of the Atacama Desert, are reported and studied by high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) for the first time. In addition, determination of the carotenoid pigment profile from the new strains of Haloarcula sp., plus two strains of Halorubrum tebenquichense, and their antioxidant activity by means of several methods is reported. The effect of biomass on cellular viability in skin cell lines was also evaluated by MTT assay. The cholinesterase inhibition capacity of six haloarchaea (Haloarcula sp. ALT-23; Haloarcula sp. TeSe-41; Haloarcula sp. TeSe-51; Haloarcula sp. Te Se-89 and Halorubrum tebenquichense strains TeSe-85 and Te Se-86) is also reported for the first time. AChE inhibition IC(50) was 2.96 ± 0.08 μg/mL and BuChE inhibition IC(50) was 2.39 ± 0.09 μg/mL for the most active strain, Halorubrum tebenquichense Te Se-85, respectively, which is more active in BuCHe than that of the standard galantamine. Docking calculation showed that carotenoids can exert their inhibitory activity fitting into the enzyme pocket by their halves, in the presence of cholinesterase dimers. MDPI 2021-07-30 /pmc/articles/PMC8389013/ /pubmed/34439478 http://dx.doi.org/10.3390/antiox10081230 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lizama, Catherine
Romero-Parra, Javier
Andrade, Daniel
Riveros, Felipe
Bórquez, Jorge
Ahmed, Shakeel
Venegas-Salas, Luis
Cabalín, Carolina
Simirgiotis, Mario J.
Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability
title Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability
title_full Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability
title_fullStr Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability
title_full_unstemmed Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability
title_short Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability
title_sort analysis of carotenoids in haloarchaea species from atacama saline lakes by high resolution uhplc-q-orbitrap-mass spectrometry: antioxidant potential and biological effect on cell viability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389013/
https://www.ncbi.nlm.nih.gov/pubmed/34439478
http://dx.doi.org/10.3390/antiox10081230
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