Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability
Haloarchaea are extreme halophilic microorganisms belonging to the domain Archaea, phylum Euryarchaeota, and are producers of interesting antioxidant carotenoid compounds. In this study, four new strains of Haloarcula sp., isolated from saline lakes of the Atacama Desert, are reported and studied by...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389013/ https://www.ncbi.nlm.nih.gov/pubmed/34439478 http://dx.doi.org/10.3390/antiox10081230 |
_version_ | 1783742766966112256 |
---|---|
author | Lizama, Catherine Romero-Parra, Javier Andrade, Daniel Riveros, Felipe Bórquez, Jorge Ahmed, Shakeel Venegas-Salas, Luis Cabalín, Carolina Simirgiotis, Mario J. |
author_facet | Lizama, Catherine Romero-Parra, Javier Andrade, Daniel Riveros, Felipe Bórquez, Jorge Ahmed, Shakeel Venegas-Salas, Luis Cabalín, Carolina Simirgiotis, Mario J. |
author_sort | Lizama, Catherine |
collection | PubMed |
description | Haloarchaea are extreme halophilic microorganisms belonging to the domain Archaea, phylum Euryarchaeota, and are producers of interesting antioxidant carotenoid compounds. In this study, four new strains of Haloarcula sp., isolated from saline lakes of the Atacama Desert, are reported and studied by high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) for the first time. In addition, determination of the carotenoid pigment profile from the new strains of Haloarcula sp., plus two strains of Halorubrum tebenquichense, and their antioxidant activity by means of several methods is reported. The effect of biomass on cellular viability in skin cell lines was also evaluated by MTT assay. The cholinesterase inhibition capacity of six haloarchaea (Haloarcula sp. ALT-23; Haloarcula sp. TeSe-41; Haloarcula sp. TeSe-51; Haloarcula sp. Te Se-89 and Halorubrum tebenquichense strains TeSe-85 and Te Se-86) is also reported for the first time. AChE inhibition IC(50) was 2.96 ± 0.08 μg/mL and BuChE inhibition IC(50) was 2.39 ± 0.09 μg/mL for the most active strain, Halorubrum tebenquichense Te Se-85, respectively, which is more active in BuCHe than that of the standard galantamine. Docking calculation showed that carotenoids can exert their inhibitory activity fitting into the enzyme pocket by their halves, in the presence of cholinesterase dimers. |
format | Online Article Text |
id | pubmed-8389013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83890132021-08-27 Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability Lizama, Catherine Romero-Parra, Javier Andrade, Daniel Riveros, Felipe Bórquez, Jorge Ahmed, Shakeel Venegas-Salas, Luis Cabalín, Carolina Simirgiotis, Mario J. Antioxidants (Basel) Article Haloarchaea are extreme halophilic microorganisms belonging to the domain Archaea, phylum Euryarchaeota, and are producers of interesting antioxidant carotenoid compounds. In this study, four new strains of Haloarcula sp., isolated from saline lakes of the Atacama Desert, are reported and studied by high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) for the first time. In addition, determination of the carotenoid pigment profile from the new strains of Haloarcula sp., plus two strains of Halorubrum tebenquichense, and their antioxidant activity by means of several methods is reported. The effect of biomass on cellular viability in skin cell lines was also evaluated by MTT assay. The cholinesterase inhibition capacity of six haloarchaea (Haloarcula sp. ALT-23; Haloarcula sp. TeSe-41; Haloarcula sp. TeSe-51; Haloarcula sp. Te Se-89 and Halorubrum tebenquichense strains TeSe-85 and Te Se-86) is also reported for the first time. AChE inhibition IC(50) was 2.96 ± 0.08 μg/mL and BuChE inhibition IC(50) was 2.39 ± 0.09 μg/mL for the most active strain, Halorubrum tebenquichense Te Se-85, respectively, which is more active in BuCHe than that of the standard galantamine. Docking calculation showed that carotenoids can exert their inhibitory activity fitting into the enzyme pocket by their halves, in the presence of cholinesterase dimers. MDPI 2021-07-30 /pmc/articles/PMC8389013/ /pubmed/34439478 http://dx.doi.org/10.3390/antiox10081230 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lizama, Catherine Romero-Parra, Javier Andrade, Daniel Riveros, Felipe Bórquez, Jorge Ahmed, Shakeel Venegas-Salas, Luis Cabalín, Carolina Simirgiotis, Mario J. Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability |
title | Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability |
title_full | Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability |
title_fullStr | Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability |
title_full_unstemmed | Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability |
title_short | Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability |
title_sort | analysis of carotenoids in haloarchaea species from atacama saline lakes by high resolution uhplc-q-orbitrap-mass spectrometry: antioxidant potential and biological effect on cell viability |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389013/ https://www.ncbi.nlm.nih.gov/pubmed/34439478 http://dx.doi.org/10.3390/antiox10081230 |
work_keys_str_mv | AT lizamacatherine analysisofcarotenoidsinhaloarchaeaspeciesfromatacamasalinelakesbyhighresolutionuhplcqorbitrapmassspectrometryantioxidantpotentialandbiologicaleffectoncellviability AT romeroparrajavier analysisofcarotenoidsinhaloarchaeaspeciesfromatacamasalinelakesbyhighresolutionuhplcqorbitrapmassspectrometryantioxidantpotentialandbiologicaleffectoncellviability AT andradedaniel analysisofcarotenoidsinhaloarchaeaspeciesfromatacamasalinelakesbyhighresolutionuhplcqorbitrapmassspectrometryantioxidantpotentialandbiologicaleffectoncellviability AT riverosfelipe analysisofcarotenoidsinhaloarchaeaspeciesfromatacamasalinelakesbyhighresolutionuhplcqorbitrapmassspectrometryantioxidantpotentialandbiologicaleffectoncellviability AT borquezjorge analysisofcarotenoidsinhaloarchaeaspeciesfromatacamasalinelakesbyhighresolutionuhplcqorbitrapmassspectrometryantioxidantpotentialandbiologicaleffectoncellviability AT ahmedshakeel analysisofcarotenoidsinhaloarchaeaspeciesfromatacamasalinelakesbyhighresolutionuhplcqorbitrapmassspectrometryantioxidantpotentialandbiologicaleffectoncellviability AT venegassalasluis analysisofcarotenoidsinhaloarchaeaspeciesfromatacamasalinelakesbyhighresolutionuhplcqorbitrapmassspectrometryantioxidantpotentialandbiologicaleffectoncellviability AT cabalincarolina analysisofcarotenoidsinhaloarchaeaspeciesfromatacamasalinelakesbyhighresolutionuhplcqorbitrapmassspectrometryantioxidantpotentialandbiologicaleffectoncellviability AT simirgiotismarioj analysisofcarotenoidsinhaloarchaeaspeciesfromatacamasalinelakesbyhighresolutionuhplcqorbitrapmassspectrometryantioxidantpotentialandbiologicaleffectoncellviability |