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Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea
Early detection of obstructive sleep apnea (OSA) is needed to reduce cardiovascular sequelae and mortality. Full-night polysomnography has been used for diagnosing OSA, but it is too expensive and inconvenient for patients to handle. Metabolome-wide analyses were performed to find and validate surro...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389315/ https://www.ncbi.nlm.nih.gov/pubmed/34439490 http://dx.doi.org/10.3390/antiox10081242 |
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author | Shin, Hyun-Woo Cho, Kumsun Rhee, Chae-Seo Hong, Il-Hee Cho, Seok Hyun Kim, Sung Wan Kim, Jiyoung So, Daeho Cho, Joo-Youn Park, Jong-Wan |
author_facet | Shin, Hyun-Woo Cho, Kumsun Rhee, Chae-Seo Hong, Il-Hee Cho, Seok Hyun Kim, Sung Wan Kim, Jiyoung So, Daeho Cho, Joo-Youn Park, Jong-Wan |
author_sort | Shin, Hyun-Woo |
collection | PubMed |
description | Early detection of obstructive sleep apnea (OSA) is needed to reduce cardiovascular sequelae and mortality. Full-night polysomnography has been used for diagnosing OSA, but it is too expensive and inconvenient for patients to handle. Metabolome-wide analyses were performed to find and validate surrogate markers for OSA. We further investigated the mechanism underlying hypoxic induction of the markers in human cells and mice. Arachidonic acid derivatives 5-HETE and 5-oxoETE were detected in urine samples. The levels (mean ± SD, ng per mg creatinine) of 5-HETE and 5-oxoETE were 56.4 ± 26.2 and 46.9 ± 18.4 in OSA patients, respectively, which were significantly higher than those in controls (22.5 ± 4.6 and 18.7 ± 3.6). Both levels correlated with the apnea-hypopnea index and the lowest oxygen saturation on polysomnography. After the treatment with the continuous positive airway pressure, the metabolite levels were significantly reduced compared with those before the treatment. In human mononuclear cells subjected to intermittent hypoxia, 5-HETE and 5-oxoETE productions were induced by hypoxia-inducible factor 1 and glutathione peroxidase. When mice were exposed to intermittent hypoxia, 5-HETE and 5-oxoETE were excreted more in urine. They were identified and verified as new OSA markers reflecting hypoxic stress. The OSA markers could be used for OSA diagnosis and therapeutic evaluation. |
format | Online Article Text |
id | pubmed-8389315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83893152021-08-27 Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea Shin, Hyun-Woo Cho, Kumsun Rhee, Chae-Seo Hong, Il-Hee Cho, Seok Hyun Kim, Sung Wan Kim, Jiyoung So, Daeho Cho, Joo-Youn Park, Jong-Wan Antioxidants (Basel) Article Early detection of obstructive sleep apnea (OSA) is needed to reduce cardiovascular sequelae and mortality. Full-night polysomnography has been used for diagnosing OSA, but it is too expensive and inconvenient for patients to handle. Metabolome-wide analyses were performed to find and validate surrogate markers for OSA. We further investigated the mechanism underlying hypoxic induction of the markers in human cells and mice. Arachidonic acid derivatives 5-HETE and 5-oxoETE were detected in urine samples. The levels (mean ± SD, ng per mg creatinine) of 5-HETE and 5-oxoETE were 56.4 ± 26.2 and 46.9 ± 18.4 in OSA patients, respectively, which were significantly higher than those in controls (22.5 ± 4.6 and 18.7 ± 3.6). Both levels correlated with the apnea-hypopnea index and the lowest oxygen saturation on polysomnography. After the treatment with the continuous positive airway pressure, the metabolite levels were significantly reduced compared with those before the treatment. In human mononuclear cells subjected to intermittent hypoxia, 5-HETE and 5-oxoETE productions were induced by hypoxia-inducible factor 1 and glutathione peroxidase. When mice were exposed to intermittent hypoxia, 5-HETE and 5-oxoETE were excreted more in urine. They were identified and verified as new OSA markers reflecting hypoxic stress. The OSA markers could be used for OSA diagnosis and therapeutic evaluation. MDPI 2021-08-03 /pmc/articles/PMC8389315/ /pubmed/34439490 http://dx.doi.org/10.3390/antiox10081242 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shin, Hyun-Woo Cho, Kumsun Rhee, Chae-Seo Hong, Il-Hee Cho, Seok Hyun Kim, Sung Wan Kim, Jiyoung So, Daeho Cho, Joo-Youn Park, Jong-Wan Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea |
title | Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea |
title_full | Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea |
title_fullStr | Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea |
title_full_unstemmed | Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea |
title_short | Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea |
title_sort | urine 5-eicosatetraenoic acids as diagnostic markers for obstructive sleep apnea |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389315/ https://www.ncbi.nlm.nih.gov/pubmed/34439490 http://dx.doi.org/10.3390/antiox10081242 |
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