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Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea

Early detection of obstructive sleep apnea (OSA) is needed to reduce cardiovascular sequelae and mortality. Full-night polysomnography has been used for diagnosing OSA, but it is too expensive and inconvenient for patients to handle. Metabolome-wide analyses were performed to find and validate surro...

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Autores principales: Shin, Hyun-Woo, Cho, Kumsun, Rhee, Chae-Seo, Hong, Il-Hee, Cho, Seok Hyun, Kim, Sung Wan, Kim, Jiyoung, So, Daeho, Cho, Joo-Youn, Park, Jong-Wan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389315/
https://www.ncbi.nlm.nih.gov/pubmed/34439490
http://dx.doi.org/10.3390/antiox10081242
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author Shin, Hyun-Woo
Cho, Kumsun
Rhee, Chae-Seo
Hong, Il-Hee
Cho, Seok Hyun
Kim, Sung Wan
Kim, Jiyoung
So, Daeho
Cho, Joo-Youn
Park, Jong-Wan
author_facet Shin, Hyun-Woo
Cho, Kumsun
Rhee, Chae-Seo
Hong, Il-Hee
Cho, Seok Hyun
Kim, Sung Wan
Kim, Jiyoung
So, Daeho
Cho, Joo-Youn
Park, Jong-Wan
author_sort Shin, Hyun-Woo
collection PubMed
description Early detection of obstructive sleep apnea (OSA) is needed to reduce cardiovascular sequelae and mortality. Full-night polysomnography has been used for diagnosing OSA, but it is too expensive and inconvenient for patients to handle. Metabolome-wide analyses were performed to find and validate surrogate markers for OSA. We further investigated the mechanism underlying hypoxic induction of the markers in human cells and mice. Arachidonic acid derivatives 5-HETE and 5-oxoETE were detected in urine samples. The levels (mean ± SD, ng per mg creatinine) of 5-HETE and 5-oxoETE were 56.4 ± 26.2 and 46.9 ± 18.4 in OSA patients, respectively, which were significantly higher than those in controls (22.5 ± 4.6 and 18.7 ± 3.6). Both levels correlated with the apnea-hypopnea index and the lowest oxygen saturation on polysomnography. After the treatment with the continuous positive airway pressure, the metabolite levels were significantly reduced compared with those before the treatment. In human mononuclear cells subjected to intermittent hypoxia, 5-HETE and 5-oxoETE productions were induced by hypoxia-inducible factor 1 and glutathione peroxidase. When mice were exposed to intermittent hypoxia, 5-HETE and 5-oxoETE were excreted more in urine. They were identified and verified as new OSA markers reflecting hypoxic stress. The OSA markers could be used for OSA diagnosis and therapeutic evaluation.
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spelling pubmed-83893152021-08-27 Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea Shin, Hyun-Woo Cho, Kumsun Rhee, Chae-Seo Hong, Il-Hee Cho, Seok Hyun Kim, Sung Wan Kim, Jiyoung So, Daeho Cho, Joo-Youn Park, Jong-Wan Antioxidants (Basel) Article Early detection of obstructive sleep apnea (OSA) is needed to reduce cardiovascular sequelae and mortality. Full-night polysomnography has been used for diagnosing OSA, but it is too expensive and inconvenient for patients to handle. Metabolome-wide analyses were performed to find and validate surrogate markers for OSA. We further investigated the mechanism underlying hypoxic induction of the markers in human cells and mice. Arachidonic acid derivatives 5-HETE and 5-oxoETE were detected in urine samples. The levels (mean ± SD, ng per mg creatinine) of 5-HETE and 5-oxoETE were 56.4 ± 26.2 and 46.9 ± 18.4 in OSA patients, respectively, which were significantly higher than those in controls (22.5 ± 4.6 and 18.7 ± 3.6). Both levels correlated with the apnea-hypopnea index and the lowest oxygen saturation on polysomnography. After the treatment with the continuous positive airway pressure, the metabolite levels were significantly reduced compared with those before the treatment. In human mononuclear cells subjected to intermittent hypoxia, 5-HETE and 5-oxoETE productions were induced by hypoxia-inducible factor 1 and glutathione peroxidase. When mice were exposed to intermittent hypoxia, 5-HETE and 5-oxoETE were excreted more in urine. They were identified and verified as new OSA markers reflecting hypoxic stress. The OSA markers could be used for OSA diagnosis and therapeutic evaluation. MDPI 2021-08-03 /pmc/articles/PMC8389315/ /pubmed/34439490 http://dx.doi.org/10.3390/antiox10081242 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shin, Hyun-Woo
Cho, Kumsun
Rhee, Chae-Seo
Hong, Il-Hee
Cho, Seok Hyun
Kim, Sung Wan
Kim, Jiyoung
So, Daeho
Cho, Joo-Youn
Park, Jong-Wan
Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea
title Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea
title_full Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea
title_fullStr Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea
title_full_unstemmed Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea
title_short Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea
title_sort urine 5-eicosatetraenoic acids as diagnostic markers for obstructive sleep apnea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389315/
https://www.ncbi.nlm.nih.gov/pubmed/34439490
http://dx.doi.org/10.3390/antiox10081242
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