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A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination
Incomplete remyelination is frequent in multiple sclerosis (MS)-lesions, but there is no established marker for recent remyelination. We investigated the role of the oligodendrocyte/myelin protein ermin in de- and remyelination in the cuprizone (CPZ) mouse model, and in MS. The density of ermin+ oli...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389439/ https://www.ncbi.nlm.nih.gov/pubmed/34437581 http://dx.doi.org/10.1371/journal.pone.0256155 |
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author | Ahmad, Intakhar Wergeland, Stig Oveland, Eystein Bø, Lars |
author_facet | Ahmad, Intakhar Wergeland, Stig Oveland, Eystein Bø, Lars |
author_sort | Ahmad, Intakhar |
collection | PubMed |
description | Incomplete remyelination is frequent in multiple sclerosis (MS)-lesions, but there is no established marker for recent remyelination. We investigated the role of the oligodendrocyte/myelin protein ermin in de- and remyelination in the cuprizone (CPZ) mouse model, and in MS. The density of ermin+ oligodendrocytes in the brain was significantly decreased after one week of CPZ exposure (p < 0.02). The relative proportion of ermin+ cells compared to cells positive for the late-stage oligodendrocyte marker Nogo-A increased at the onset of remyelination in the corpus callosum (p < 0.02). The density of ermin-positive cells increased in the corpus callosum during the CPZ-phase of extensive remyelination (p < 0.0001). In MS, the density of ermin+ cells was higher in remyelinated lesion areas compared to non-remyelinated areas both in white- (p < 0.0001) and grey matter (p < 0.0001) and compared to normal-appearing white matter (p < 0.001). Ermin immunopositive cells in MS-lesions were not immunopositive for the early-stage oligodendrocyte markers O4 and O1, but a subpopulation was immunopositive for Nogo-A. The data suggest a relatively higher proportion of ermin immunopositivity in oligodendrocytes compared to Nogo-A indicates recent or ongoing remyelination. |
format | Online Article Text |
id | pubmed-8389439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-83894392021-08-27 A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination Ahmad, Intakhar Wergeland, Stig Oveland, Eystein Bø, Lars PLoS One Research Article Incomplete remyelination is frequent in multiple sclerosis (MS)-lesions, but there is no established marker for recent remyelination. We investigated the role of the oligodendrocyte/myelin protein ermin in de- and remyelination in the cuprizone (CPZ) mouse model, and in MS. The density of ermin+ oligodendrocytes in the brain was significantly decreased after one week of CPZ exposure (p < 0.02). The relative proportion of ermin+ cells compared to cells positive for the late-stage oligodendrocyte marker Nogo-A increased at the onset of remyelination in the corpus callosum (p < 0.02). The density of ermin-positive cells increased in the corpus callosum during the CPZ-phase of extensive remyelination (p < 0.0001). In MS, the density of ermin+ cells was higher in remyelinated lesion areas compared to non-remyelinated areas both in white- (p < 0.0001) and grey matter (p < 0.0001) and compared to normal-appearing white matter (p < 0.001). Ermin immunopositive cells in MS-lesions were not immunopositive for the early-stage oligodendrocyte markers O4 and O1, but a subpopulation was immunopositive for Nogo-A. The data suggest a relatively higher proportion of ermin immunopositivity in oligodendrocytes compared to Nogo-A indicates recent or ongoing remyelination. Public Library of Science 2021-08-26 /pmc/articles/PMC8389439/ /pubmed/34437581 http://dx.doi.org/10.1371/journal.pone.0256155 Text en © 2021 Ahmad et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ahmad, Intakhar Wergeland, Stig Oveland, Eystein Bø, Lars A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination |
title | A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination |
title_full | A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination |
title_fullStr | A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination |
title_full_unstemmed | A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination |
title_short | A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination |
title_sort | higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389439/ https://www.ncbi.nlm.nih.gov/pubmed/34437581 http://dx.doi.org/10.1371/journal.pone.0256155 |
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