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A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination

Incomplete remyelination is frequent in multiple sclerosis (MS)-lesions, but there is no established marker for recent remyelination. We investigated the role of the oligodendrocyte/myelin protein ermin in de- and remyelination in the cuprizone (CPZ) mouse model, and in MS. The density of ermin+ oli...

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Autores principales: Ahmad, Intakhar, Wergeland, Stig, Oveland, Eystein, Bø, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389439/
https://www.ncbi.nlm.nih.gov/pubmed/34437581
http://dx.doi.org/10.1371/journal.pone.0256155
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author Ahmad, Intakhar
Wergeland, Stig
Oveland, Eystein
Bø, Lars
author_facet Ahmad, Intakhar
Wergeland, Stig
Oveland, Eystein
Bø, Lars
author_sort Ahmad, Intakhar
collection PubMed
description Incomplete remyelination is frequent in multiple sclerosis (MS)-lesions, but there is no established marker for recent remyelination. We investigated the role of the oligodendrocyte/myelin protein ermin in de- and remyelination in the cuprizone (CPZ) mouse model, and in MS. The density of ermin+ oligodendrocytes in the brain was significantly decreased after one week of CPZ exposure (p < 0.02). The relative proportion of ermin+ cells compared to cells positive for the late-stage oligodendrocyte marker Nogo-A increased at the onset of remyelination in the corpus callosum (p < 0.02). The density of ermin-positive cells increased in the corpus callosum during the CPZ-phase of extensive remyelination (p < 0.0001). In MS, the density of ermin+ cells was higher in remyelinated lesion areas compared to non-remyelinated areas both in white- (p < 0.0001) and grey matter (p < 0.0001) and compared to normal-appearing white matter (p < 0.001). Ermin immunopositive cells in MS-lesions were not immunopositive for the early-stage oligodendrocyte markers O4 and O1, but a subpopulation was immunopositive for Nogo-A. The data suggest a relatively higher proportion of ermin immunopositivity in oligodendrocytes compared to Nogo-A indicates recent or ongoing remyelination.
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spelling pubmed-83894392021-08-27 A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination Ahmad, Intakhar Wergeland, Stig Oveland, Eystein Bø, Lars PLoS One Research Article Incomplete remyelination is frequent in multiple sclerosis (MS)-lesions, but there is no established marker for recent remyelination. We investigated the role of the oligodendrocyte/myelin protein ermin in de- and remyelination in the cuprizone (CPZ) mouse model, and in MS. The density of ermin+ oligodendrocytes in the brain was significantly decreased after one week of CPZ exposure (p < 0.02). The relative proportion of ermin+ cells compared to cells positive for the late-stage oligodendrocyte marker Nogo-A increased at the onset of remyelination in the corpus callosum (p < 0.02). The density of ermin-positive cells increased in the corpus callosum during the CPZ-phase of extensive remyelination (p < 0.0001). In MS, the density of ermin+ cells was higher in remyelinated lesion areas compared to non-remyelinated areas both in white- (p < 0.0001) and grey matter (p < 0.0001) and compared to normal-appearing white matter (p < 0.001). Ermin immunopositive cells in MS-lesions were not immunopositive for the early-stage oligodendrocyte markers O4 and O1, but a subpopulation was immunopositive for Nogo-A. The data suggest a relatively higher proportion of ermin immunopositivity in oligodendrocytes compared to Nogo-A indicates recent or ongoing remyelination. Public Library of Science 2021-08-26 /pmc/articles/PMC8389439/ /pubmed/34437581 http://dx.doi.org/10.1371/journal.pone.0256155 Text en © 2021 Ahmad et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ahmad, Intakhar
Wergeland, Stig
Oveland, Eystein
Bø, Lars
A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination
title A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination
title_full A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination
title_fullStr A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination
title_full_unstemmed A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination
title_short A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination
title_sort higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389439/
https://www.ncbi.nlm.nih.gov/pubmed/34437581
http://dx.doi.org/10.1371/journal.pone.0256155
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