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Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments

Dental procedures produce a large amount of spatter and aerosols that create concern for the transmission of airborne diseases, such as Covid-19. This study established a methodology with the objective of evaluating new associated strategies to reduce the risk of cross-transmission in a health envir...

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Autores principales: Montalli, Victor Angelo Martins, de Freitas, Patrícia Rejane, Torres, Milenna de Figueiredo, Torres Junior, Oscar de Figueiredo, Vilhena, Dienne Hellen Moutinho De, Junqueira, José Luiz Cintra, Napimoga, Marcelo Henrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389494/
https://www.ncbi.nlm.nih.gov/pubmed/34437589
http://dx.doi.org/10.1371/journal.pone.0255533
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author Montalli, Victor Angelo Martins
de Freitas, Patrícia Rejane
Torres, Milenna de Figueiredo
Torres Junior, Oscar de Figueiredo
Vilhena, Dienne Hellen Moutinho De
Junqueira, José Luiz Cintra
Napimoga, Marcelo Henrique
author_facet Montalli, Victor Angelo Martins
de Freitas, Patrícia Rejane
Torres, Milenna de Figueiredo
Torres Junior, Oscar de Figueiredo
Vilhena, Dienne Hellen Moutinho De
Junqueira, José Luiz Cintra
Napimoga, Marcelo Henrique
author_sort Montalli, Victor Angelo Martins
collection PubMed
description Dental procedures produce a large amount of spatter and aerosols that create concern for the transmission of airborne diseases, such as Covid-19. This study established a methodology with the objective of evaluating new associated strategies to reduce the risk of cross-transmission in a health environment by simulating spread of potentially contaminated dispersion particles (PCDP) in the environment. This crossover study, was conducted in a school clinic environment (4 clinics containing 12 dental chairs each). As a positive control group (without barriers), 12 professionals activated at the same time the turbine of dental drill, for one minute, with a bacterial solution (Lactobacillus casei Shirota, 1.5x10(8) CFU/mL), which had been added in the cooling reservoir of the dental equipment. In the experimental groups, the professionals made use of; a) an individual biosafety barrier in dentistry (IBBD) which consists of a metal support covered by a disposable PVC film barrier; b) a Mobile Unit of Disinfection by Ultraviolet-C, consisting of 8 UV lamps-C of 95W, of 304μW/cm(2) of irradiance each, connected for 15 minutes (UV-C) and; c) the association between the two methods (IBBD + UV-C). In each clinic, 56 Petri dishes containing MRS agar were positioned on the lamps, benches and on the floor. In addition, plates were placed prior to each test (negative control group) and plates were also placed in the corridor that connects the four clinics. In the groups without barrier and IBBD + UV-C the passive air microorganisms in Petri dishes was also evaluated at times of 30, 60, 90 and 120 minutes after the end of the dental’s drill activation. The mean (standard deviation) of CFU of L. casei Shirota for the positive control group was 3905 (1521), while in the experimental groups the mean using the IBBD was 940 (466) CFU, establishing a reduction on average, of 75% (p<0.0001). For the UV-C group, the mean was 260 (309) CFU and the association of the use of IBBD + UV-C promoted an overall average count of 152 (257) CFU, establishing a reduction on average of 93% and 96%, respectively (p<0.0001). Considering these results and the study model used, the individual biosafety barrier associated with UV-C technology showed to be efficient strategies to reduce the dispersion of bioaerosols generated in an environment with high rate of PCDP generation and may be an alternative for the improvement of biosafety in different healthy environment.
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spelling pubmed-83894942021-08-27 Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments Montalli, Victor Angelo Martins de Freitas, Patrícia Rejane Torres, Milenna de Figueiredo Torres Junior, Oscar de Figueiredo Vilhena, Dienne Hellen Moutinho De Junqueira, José Luiz Cintra Napimoga, Marcelo Henrique PLoS One Research Article Dental procedures produce a large amount of spatter and aerosols that create concern for the transmission of airborne diseases, such as Covid-19. This study established a methodology with the objective of evaluating new associated strategies to reduce the risk of cross-transmission in a health environment by simulating spread of potentially contaminated dispersion particles (PCDP) in the environment. This crossover study, was conducted in a school clinic environment (4 clinics containing 12 dental chairs each). As a positive control group (without barriers), 12 professionals activated at the same time the turbine of dental drill, for one minute, with a bacterial solution (Lactobacillus casei Shirota, 1.5x10(8) CFU/mL), which had been added in the cooling reservoir of the dental equipment. In the experimental groups, the professionals made use of; a) an individual biosafety barrier in dentistry (IBBD) which consists of a metal support covered by a disposable PVC film barrier; b) a Mobile Unit of Disinfection by Ultraviolet-C, consisting of 8 UV lamps-C of 95W, of 304μW/cm(2) of irradiance each, connected for 15 minutes (UV-C) and; c) the association between the two methods (IBBD + UV-C). In each clinic, 56 Petri dishes containing MRS agar were positioned on the lamps, benches and on the floor. In addition, plates were placed prior to each test (negative control group) and plates were also placed in the corridor that connects the four clinics. In the groups without barrier and IBBD + UV-C the passive air microorganisms in Petri dishes was also evaluated at times of 30, 60, 90 and 120 minutes after the end of the dental’s drill activation. The mean (standard deviation) of CFU of L. casei Shirota for the positive control group was 3905 (1521), while in the experimental groups the mean using the IBBD was 940 (466) CFU, establishing a reduction on average, of 75% (p<0.0001). For the UV-C group, the mean was 260 (309) CFU and the association of the use of IBBD + UV-C promoted an overall average count of 152 (257) CFU, establishing a reduction on average of 93% and 96%, respectively (p<0.0001). Considering these results and the study model used, the individual biosafety barrier associated with UV-C technology showed to be efficient strategies to reduce the dispersion of bioaerosols generated in an environment with high rate of PCDP generation and may be an alternative for the improvement of biosafety in different healthy environment. Public Library of Science 2021-08-26 /pmc/articles/PMC8389494/ /pubmed/34437589 http://dx.doi.org/10.1371/journal.pone.0255533 Text en © 2021 Montalli et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Montalli, Victor Angelo Martins
de Freitas, Patrícia Rejane
Torres, Milenna de Figueiredo
Torres Junior, Oscar de Figueiredo
Vilhena, Dienne Hellen Moutinho De
Junqueira, José Luiz Cintra
Napimoga, Marcelo Henrique
Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments
title Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments
title_full Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments
title_fullStr Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments
title_full_unstemmed Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments
title_short Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments
title_sort biosafety devices to control the spread of potentially contaminated dispersion particles. new associated strategies for health environments
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389494/
https://www.ncbi.nlm.nih.gov/pubmed/34437589
http://dx.doi.org/10.1371/journal.pone.0255533
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