Phosphorus-Induced Adaptation Mechanisms of Rye Grown on Post-Flotation Copper Tailings

SIMPLE SUMMARY: The mining activities for the production of copper, lead, zinc, and others are inevitably associated with the generation of an enormous volume of waste materials, i.e., tailings. The global area covered with tailings is on the order of hundreds of millions of hectares, with this being predicted to rise significantly in the coming decades. Importantly, the physicochemical properties of tailings, such as high content of heavy metals and high pH, pose a serious threat to the surrounding ecological environment. This, combined with low available nutrients, makes revegetation of degraded lands very difficult, with the number of field trials demonstrating successful revitalisation remaining very low. In this study, we investigate in rye, as a model plant, a wide array of physiological processes and their significance in determining survival on the copper tailings. We demonstrate that limitations in plant growth on such wastes is not simply related to high copper content. Rather, we present data that the low availability of phosphorus and activity of the mechanisms involved in phosphorus extraction from the rhizosphere are important determinants of the plant growth and survival rate. With these results, we make a direct and significant contribution towards meeting future demands for effective revitalisation techniques of degraded lands. ABSTRACT: Although a considerable effort has been made over the last decades to develop cost-effective phytotechnologies as an alternative to conventional techniques for the management of contaminated lands, successful revegetation of the tailings still represents a major challenge. Here, we evaluate the potential of rye (Secale cereale L.) for growth and survival on the tailings after copper (Cu) ore processing. Four rye varieties were cultivated in a pot experiment on the post-flotation sediment with increasing phosphorus (P) doses (22, 44, 66, 88, and 110 mg·kg(−1)). The resistance of the studied rye genotypes to stress was assessed by observing the growth and development of plants, determining the dry mass accumulation, the Cu and P uptake and content, and a number of physiological parameters related mainly to P mobilisation. Exposure of tested rye varieties to high Cu concentrations in the tailings did not result in any significant plant mortality, with the intracellular Cu concentrations being below the critical toxic level. In contrast, the low availability of P due to alkaline properties of the tailings and the mechanisms involved in the mobilisation of sparingly soluble forms of this element (i.e., H(+)-ATPase-driven proton efflux in roots and organic acid exudation), were identified as main factor determining the level of tolerance. The efficiency of the photosynthetic activity was a key determinant for the P-mobilising capacity of rye. We further showed that rye varieties with more primitive genetic background might be potentially more suitable for growth on the post-flotation copper tailings. The results provide important and novel knowledge that will certainly support future works in developing strategies for successful revitalisation of degraded lands.