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Predictive Antibiotic Susceptibility Testing by Next-Generation Sequencing for Periprosthetic Joint Infections: Potential and Limitations

Joint replacement surgeries are one of the most frequent medical interventions globally. Infections of prosthetic joints are a major health challenge and typically require prolonged or even indefinite antibiotic treatment. As multidrug-resistant pathogens continue to rise globally, novel diagnostics...

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Autores principales: Lüftinger, Lukas, Ferreira, Ines, Frank, Bernhard J. H., Beisken, Stephan, Weinberger, Johannes, von Haeseler, Arndt, Rattei, Thomas, Hofstaetter, Jochen G., Posch, Andreas E., Materna, Arne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389688/
https://www.ncbi.nlm.nih.gov/pubmed/34440114
http://dx.doi.org/10.3390/biomedicines9080910
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author Lüftinger, Lukas
Ferreira, Ines
Frank, Bernhard J. H.
Beisken, Stephan
Weinberger, Johannes
von Haeseler, Arndt
Rattei, Thomas
Hofstaetter, Jochen G.
Posch, Andreas E.
Materna, Arne
author_facet Lüftinger, Lukas
Ferreira, Ines
Frank, Bernhard J. H.
Beisken, Stephan
Weinberger, Johannes
von Haeseler, Arndt
Rattei, Thomas
Hofstaetter, Jochen G.
Posch, Andreas E.
Materna, Arne
author_sort Lüftinger, Lukas
collection PubMed
description Joint replacement surgeries are one of the most frequent medical interventions globally. Infections of prosthetic joints are a major health challenge and typically require prolonged or even indefinite antibiotic treatment. As multidrug-resistant pathogens continue to rise globally, novel diagnostics are critical to ensure appropriate treatment and help with prosthetic joint infections (PJI) management. To this end, recent studies have shown the potential of molecular methods such as next-generation sequencing to complement established phenotypic, culture-based methods. Together with advanced bioinformatics approaches, next-generation sequencing can provide comprehensive information on pathogen identity as well as antimicrobial susceptibility, potentially enabling rapid diagnosis and targeted therapy of PJIs. In this review, we summarize current developments in next generation sequencing based predictive antibiotic susceptibility testing and discuss potential and limitations for common PJI pathogens.
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spelling pubmed-83896882021-08-27 Predictive Antibiotic Susceptibility Testing by Next-Generation Sequencing for Periprosthetic Joint Infections: Potential and Limitations Lüftinger, Lukas Ferreira, Ines Frank, Bernhard J. H. Beisken, Stephan Weinberger, Johannes von Haeseler, Arndt Rattei, Thomas Hofstaetter, Jochen G. Posch, Andreas E. Materna, Arne Biomedicines Review Joint replacement surgeries are one of the most frequent medical interventions globally. Infections of prosthetic joints are a major health challenge and typically require prolonged or even indefinite antibiotic treatment. As multidrug-resistant pathogens continue to rise globally, novel diagnostics are critical to ensure appropriate treatment and help with prosthetic joint infections (PJI) management. To this end, recent studies have shown the potential of molecular methods such as next-generation sequencing to complement established phenotypic, culture-based methods. Together with advanced bioinformatics approaches, next-generation sequencing can provide comprehensive information on pathogen identity as well as antimicrobial susceptibility, potentially enabling rapid diagnosis and targeted therapy of PJIs. In this review, we summarize current developments in next generation sequencing based predictive antibiotic susceptibility testing and discuss potential and limitations for common PJI pathogens. MDPI 2021-07-29 /pmc/articles/PMC8389688/ /pubmed/34440114 http://dx.doi.org/10.3390/biomedicines9080910 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Lüftinger, Lukas
Ferreira, Ines
Frank, Bernhard J. H.
Beisken, Stephan
Weinberger, Johannes
von Haeseler, Arndt
Rattei, Thomas
Hofstaetter, Jochen G.
Posch, Andreas E.
Materna, Arne
Predictive Antibiotic Susceptibility Testing by Next-Generation Sequencing for Periprosthetic Joint Infections: Potential and Limitations
title Predictive Antibiotic Susceptibility Testing by Next-Generation Sequencing for Periprosthetic Joint Infections: Potential and Limitations
title_full Predictive Antibiotic Susceptibility Testing by Next-Generation Sequencing for Periprosthetic Joint Infections: Potential and Limitations
title_fullStr Predictive Antibiotic Susceptibility Testing by Next-Generation Sequencing for Periprosthetic Joint Infections: Potential and Limitations
title_full_unstemmed Predictive Antibiotic Susceptibility Testing by Next-Generation Sequencing for Periprosthetic Joint Infections: Potential and Limitations
title_short Predictive Antibiotic Susceptibility Testing by Next-Generation Sequencing for Periprosthetic Joint Infections: Potential and Limitations
title_sort predictive antibiotic susceptibility testing by next-generation sequencing for periprosthetic joint infections: potential and limitations
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389688/
https://www.ncbi.nlm.nih.gov/pubmed/34440114
http://dx.doi.org/10.3390/biomedicines9080910
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