A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells

For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures. Here, a simple and tissue culture infectious dose-deriv...

Descripción completa

Detalles Bibliográficos
Autores principales: Hasler, Fabio, Duda, Agathe, Kündig, Thomas M., Johansen, Pål
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390364/
https://www.ncbi.nlm.nih.gov/pubmed/34467223
http://dx.doi.org/10.1016/j.xpro.2021.100824
_version_ 1783743072924860416
author Hasler, Fabio
Duda, Agathe
Kündig, Thomas M.
Johansen, Pål
author_facet Hasler, Fabio
Duda, Agathe
Kündig, Thomas M.
Johansen, Pål
author_sort Hasler, Fabio
collection PubMed
description For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures. Here, a simple and tissue culture infectious dose-derived protocol for assessment of neutralization of SARS-CoV-2 is described. Compared with the often applied plaque-forming unit assay, the working load is lower, and fewer manipulations of the infected cultures are required. Hence, the method is safer for the personnel.
format Online
Article
Text
id pubmed-8390364
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-83903642021-08-27 A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells Hasler, Fabio Duda, Agathe Kündig, Thomas M. Johansen, Pål STAR Protoc Protocol For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures. Here, a simple and tissue culture infectious dose-derived protocol for assessment of neutralization of SARS-CoV-2 is described. Compared with the often applied plaque-forming unit assay, the working load is lower, and fewer manipulations of the infected cultures are required. Hence, the method is safer for the personnel. Elsevier 2021-08-27 /pmc/articles/PMC8390364/ /pubmed/34467223 http://dx.doi.org/10.1016/j.xpro.2021.100824 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Hasler, Fabio
Duda, Agathe
Kündig, Thomas M.
Johansen, Pål
A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells
title A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells
title_full A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells
title_fullStr A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells
title_full_unstemmed A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells
title_short A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells
title_sort tissue culture infectious dose-derived protocol for testing of sars-cov-2 neutralization of serum antibodies on adherent cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390364/
https://www.ncbi.nlm.nih.gov/pubmed/34467223
http://dx.doi.org/10.1016/j.xpro.2021.100824
work_keys_str_mv AT haslerfabio atissuecultureinfectiousdosederivedprotocolfortestingofsarscov2neutralizationofserumantibodiesonadherentcells
AT dudaagathe atissuecultureinfectiousdosederivedprotocolfortestingofsarscov2neutralizationofserumantibodiesonadherentcells
AT kundigthomasm atissuecultureinfectiousdosederivedprotocolfortestingofsarscov2neutralizationofserumantibodiesonadherentcells
AT johansenpal atissuecultureinfectiousdosederivedprotocolfortestingofsarscov2neutralizationofserumantibodiesonadherentcells
AT haslerfabio tissuecultureinfectiousdosederivedprotocolfortestingofsarscov2neutralizationofserumantibodiesonadherentcells
AT dudaagathe tissuecultureinfectiousdosederivedprotocolfortestingofsarscov2neutralizationofserumantibodiesonadherentcells
AT kundigthomasm tissuecultureinfectiousdosederivedprotocolfortestingofsarscov2neutralizationofserumantibodiesonadherentcells
AT johansenpal tissuecultureinfectiousdosederivedprotocolfortestingofsarscov2neutralizationofserumantibodiesonadherentcells

Ejemplares similares