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Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO(2) reduction to formate

ABSTRACT: In times of global climate change and the fear of dwindling resources, we are facing different considerable challenges such as the replacement of fossil fuel–based energy carriers with the coincident maintenance of the increasing energy supply of our growing world population. Therefore, CO...

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Autores principales: Leo, Felix, Schwarz, Fabian M., Schuchmann, Kai, Müller, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390402/
https://www.ncbi.nlm.nih.gov/pubmed/34331557
http://dx.doi.org/10.1007/s00253-021-11463-z
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author Leo, Felix
Schwarz, Fabian M.
Schuchmann, Kai
Müller, Volker
author_facet Leo, Felix
Schwarz, Fabian M.
Schuchmann, Kai
Müller, Volker
author_sort Leo, Felix
collection PubMed
description ABSTRACT: In times of global climate change and the fear of dwindling resources, we are facing different considerable challenges such as the replacement of fossil fuel–based energy carriers with the coincident maintenance of the increasing energy supply of our growing world population. Therefore, CO(2) capturing and H(2) storing solutions are urgently needed. In this study, we demonstrate the production of a functional and biotechnological interesting enzyme complex from acetogenic bacteria, the hydrogen-dependent CO(2) reductase (HDCR), in the well-known model organism Escherichia coli. We identified the metabolic bottlenecks of the host organisms for the production of the HDCR enzyme complex. Here we show that the recombinant expression of a heterologous enzyme complex transforms E. coli into a whole-cell biocatalyst for hydrogen-driven CO(2) reduction to formate without the need of any external co-factors or endogenous enzymes in the reaction process. This shifts the industrial platform organism E. coli more and more into the focus as biocatalyst for CO(2)-capturing and H(2)-storage. KEY POINTS: • A functional HDCR enzyme complex was heterologously produced in E. coli. • The metabolic bottlenecks for HDCR production were identified. • HDCR enabled E. coli cell to capture and store H(2) and CO(2) in the form of formate. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-021-11463-z.
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spelling pubmed-83904022021-09-14 Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO(2) reduction to formate Leo, Felix Schwarz, Fabian M. Schuchmann, Kai Müller, Volker Appl Microbiol Biotechnol Biotechnologically Relevant Enzymes and Proteins ABSTRACT: In times of global climate change and the fear of dwindling resources, we are facing different considerable challenges such as the replacement of fossil fuel–based energy carriers with the coincident maintenance of the increasing energy supply of our growing world population. Therefore, CO(2) capturing and H(2) storing solutions are urgently needed. In this study, we demonstrate the production of a functional and biotechnological interesting enzyme complex from acetogenic bacteria, the hydrogen-dependent CO(2) reductase (HDCR), in the well-known model organism Escherichia coli. We identified the metabolic bottlenecks of the host organisms for the production of the HDCR enzyme complex. Here we show that the recombinant expression of a heterologous enzyme complex transforms E. coli into a whole-cell biocatalyst for hydrogen-driven CO(2) reduction to formate without the need of any external co-factors or endogenous enzymes in the reaction process. This shifts the industrial platform organism E. coli more and more into the focus as biocatalyst for CO(2)-capturing and H(2)-storage. KEY POINTS: • A functional HDCR enzyme complex was heterologously produced in E. coli. • The metabolic bottlenecks for HDCR production were identified. • HDCR enabled E. coli cell to capture and store H(2) and CO(2) in the form of formate. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-021-11463-z. Springer Berlin Heidelberg 2021-07-31 2021 /pmc/articles/PMC8390402/ /pubmed/34331557 http://dx.doi.org/10.1007/s00253-021-11463-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biotechnologically Relevant Enzymes and Proteins
Leo, Felix
Schwarz, Fabian M.
Schuchmann, Kai
Müller, Volker
Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO(2) reduction to formate
title Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO(2) reduction to formate
title_full Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO(2) reduction to formate
title_fullStr Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO(2) reduction to formate
title_full_unstemmed Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO(2) reduction to formate
title_short Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO(2) reduction to formate
title_sort capture of carbon dioxide and hydrogen by engineered escherichia coli: hydrogen-dependent co(2) reduction to formate
topic Biotechnologically Relevant Enzymes and Proteins
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390402/
https://www.ncbi.nlm.nih.gov/pubmed/34331557
http://dx.doi.org/10.1007/s00253-021-11463-z
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