Cargando…
OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human
Understanding the molecular underpinnings of pluripotency is a prerequisite for optimal maintenance and application of embryonic stem cells (ESCs). While the protein-protein interactions of core pluripotency factors have been identified in mouse ESCs, their interactome in human ESCs (hESCs) has not...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390644/ https://www.ncbi.nlm.nih.gov/pubmed/34446700 http://dx.doi.org/10.1038/s41467-021-25107-3 |
_version_ | 1783743119999631360 |
---|---|
author | Huang, Xin Park, Kyoung-mi Gontarz, Paul Zhang, Bo Pan, Joshua McKenzie, Zachary Fischer, Laura A. Dong, Chen Dietmann, Sabine Xing, Xiaoyun Shliaha, Pavel V. Yang, Jihong Li, Dan Ding, Junjun Lungjangwa, Tenzin Mitalipova, Maya Khan, Shafqat A. Imsoonthornruksa, Sumeth Jensen, Nick Wang, Ting Kadoch, Cigall Jaenisch, Rudolf Wang, Jianlong Theunissen, Thorold W. |
author_facet | Huang, Xin Park, Kyoung-mi Gontarz, Paul Zhang, Bo Pan, Joshua McKenzie, Zachary Fischer, Laura A. Dong, Chen Dietmann, Sabine Xing, Xiaoyun Shliaha, Pavel V. Yang, Jihong Li, Dan Ding, Junjun Lungjangwa, Tenzin Mitalipova, Maya Khan, Shafqat A. Imsoonthornruksa, Sumeth Jensen, Nick Wang, Ting Kadoch, Cigall Jaenisch, Rudolf Wang, Jianlong Theunissen, Thorold W. |
author_sort | Huang, Xin |
collection | PubMed |
description | Understanding the molecular underpinnings of pluripotency is a prerequisite for optimal maintenance and application of embryonic stem cells (ESCs). While the protein-protein interactions of core pluripotency factors have been identified in mouse ESCs, their interactome in human ESCs (hESCs) has not to date been explored. Here we mapped the OCT4 interactomes in naïve and primed hESCs, revealing extensive connections to mammalian ATP-dependent nucleosome remodeling complexes. In naïve hESCs, OCT4 is associated with both BRG1 and BRM, the two paralog ATPases of the BAF complex. Genome-wide location analyses and genetic studies reveal that these two enzymes cooperate in a functionally redundant manner in the transcriptional regulation of blastocyst-specific genes. In contrast, in primed hESCs, OCT4 cooperates with BRG1 and SOX2 to promote chromatin accessibility at ectodermal genes. This work reveals how a common transcription factor utilizes differential BAF complexes to control distinct transcriptional programs in naïve and primed hESCs. |
format | Online Article Text |
id | pubmed-8390644 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83906442021-09-22 OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human Huang, Xin Park, Kyoung-mi Gontarz, Paul Zhang, Bo Pan, Joshua McKenzie, Zachary Fischer, Laura A. Dong, Chen Dietmann, Sabine Xing, Xiaoyun Shliaha, Pavel V. Yang, Jihong Li, Dan Ding, Junjun Lungjangwa, Tenzin Mitalipova, Maya Khan, Shafqat A. Imsoonthornruksa, Sumeth Jensen, Nick Wang, Ting Kadoch, Cigall Jaenisch, Rudolf Wang, Jianlong Theunissen, Thorold W. Nat Commun Article Understanding the molecular underpinnings of pluripotency is a prerequisite for optimal maintenance and application of embryonic stem cells (ESCs). While the protein-protein interactions of core pluripotency factors have been identified in mouse ESCs, their interactome in human ESCs (hESCs) has not to date been explored. Here we mapped the OCT4 interactomes in naïve and primed hESCs, revealing extensive connections to mammalian ATP-dependent nucleosome remodeling complexes. In naïve hESCs, OCT4 is associated with both BRG1 and BRM, the two paralog ATPases of the BAF complex. Genome-wide location analyses and genetic studies reveal that these two enzymes cooperate in a functionally redundant manner in the transcriptional regulation of blastocyst-specific genes. In contrast, in primed hESCs, OCT4 cooperates with BRG1 and SOX2 to promote chromatin accessibility at ectodermal genes. This work reveals how a common transcription factor utilizes differential BAF complexes to control distinct transcriptional programs in naïve and primed hESCs. Nature Publishing Group UK 2021-08-26 /pmc/articles/PMC8390644/ /pubmed/34446700 http://dx.doi.org/10.1038/s41467-021-25107-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Huang, Xin Park, Kyoung-mi Gontarz, Paul Zhang, Bo Pan, Joshua McKenzie, Zachary Fischer, Laura A. Dong, Chen Dietmann, Sabine Xing, Xiaoyun Shliaha, Pavel V. Yang, Jihong Li, Dan Ding, Junjun Lungjangwa, Tenzin Mitalipova, Maya Khan, Shafqat A. Imsoonthornruksa, Sumeth Jensen, Nick Wang, Ting Kadoch, Cigall Jaenisch, Rudolf Wang, Jianlong Theunissen, Thorold W. OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
title | OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
title_full | OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
title_fullStr | OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
title_full_unstemmed | OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
title_short | OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human |
title_sort | oct4 cooperates with distinct atp-dependent chromatin remodelers in naïve and primed pluripotent states in human |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390644/ https://www.ncbi.nlm.nih.gov/pubmed/34446700 http://dx.doi.org/10.1038/s41467-021-25107-3 |
work_keys_str_mv | AT huangxin oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT parkkyoungmi oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT gontarzpaul oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT zhangbo oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT panjoshua oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT mckenziezachary oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT fischerlauraa oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT dongchen oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT dietmannsabine oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT xingxiaoyun oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT shliahapavelv oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT yangjihong oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT lidan oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT dingjunjun oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT lungjangwatenzin oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT mitalipovamaya oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT khanshafqata oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT imsoonthornruksasumeth oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT jensennick oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT wangting oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT kadochcigall oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT jaenischrudolf oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT wangjianlong oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman AT theunissenthoroldw oct4cooperateswithdistinctatpdependentchromatinremodelersinnaiveandprimedpluripotentstatesinhuman |