Sequential staining of HIV gp140 to capture antigen-specific human B cells via flow cytometry

Protocols for efficient capture of antigen-specific B cells (ASBCs) are useful for understanding pathogen-specific B-cell responses during natural infection or vaccination. Fluorescently labeled tetramerized probes are classically used to capture ASBCs, but many occlude valuable epitopes available f...

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Detalles Bibliográficos
Autores principales: Townsley, Samantha M., Leggat, David J., Prabhakaran, Madhu, McDermott, Adrian B., Krebs, Shelly J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390768/
https://www.ncbi.nlm.nih.gov/pubmed/34471908
http://dx.doi.org/10.1016/j.xpro.2021.100771

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