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Mailing viable mouse freeze-dried spermatozoa on postcards

Freeze-drying techniques allow the preservation of mammalian spermatozoa without using liquid nitrogen. However, the current method requires the use of glass ampoules, which are breakable, expensive, and bulky to store or transport. In this study, we evaluated whether mouse freeze-dried (FD) spermat...

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Detalles Bibliográficos
Autores principales: Ito, Daiyu, Wakayama, Sayaka, Emura, Rina, Ooga, Masatoshi, Wakayama, Teruhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390851/
https://www.ncbi.nlm.nih.gov/pubmed/34471856
http://dx.doi.org/10.1016/j.isci.2021.102815
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author Ito, Daiyu
Wakayama, Sayaka
Emura, Rina
Ooga, Masatoshi
Wakayama, Teruhiko
author_facet Ito, Daiyu
Wakayama, Sayaka
Emura, Rina
Ooga, Masatoshi
Wakayama, Teruhiko
author_sort Ito, Daiyu
collection PubMed
description Freeze-drying techniques allow the preservation of mammalian spermatozoa without using liquid nitrogen. However, the current method requires the use of glass ampoules, which are breakable, expensive, and bulky to store or transport. In this study, we evaluated whether mouse freeze-dried (FD) spermatozoa can be preserved and transported on thin materials. In this study, we demonstrated that FD sperm can be preserved in thin plastic sheets. Its DNA integrity was comparable to that of glass ampoule spermatozoa, and healthy offspring were obtained after preservation at −30°C for more than 3 months. We attached preserved FD sperm to postcards, and transported these to other laboratory inexpensively at room temperatures without any protection. This method will facilitate the preservation of thousands of mouse strains in a single card holder, promote collaboration between laboratories, conservation of genetic resources, and assisted reproductive technology.
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spelling pubmed-83908512021-08-31 Mailing viable mouse freeze-dried spermatozoa on postcards Ito, Daiyu Wakayama, Sayaka Emura, Rina Ooga, Masatoshi Wakayama, Teruhiko iScience Article Freeze-drying techniques allow the preservation of mammalian spermatozoa without using liquid nitrogen. However, the current method requires the use of glass ampoules, which are breakable, expensive, and bulky to store or transport. In this study, we evaluated whether mouse freeze-dried (FD) spermatozoa can be preserved and transported on thin materials. In this study, we demonstrated that FD sperm can be preserved in thin plastic sheets. Its DNA integrity was comparable to that of glass ampoule spermatozoa, and healthy offspring were obtained after preservation at −30°C for more than 3 months. We attached preserved FD sperm to postcards, and transported these to other laboratory inexpensively at room temperatures without any protection. This method will facilitate the preservation of thousands of mouse strains in a single card holder, promote collaboration between laboratories, conservation of genetic resources, and assisted reproductive technology. Elsevier 2021-08-05 /pmc/articles/PMC8390851/ /pubmed/34471856 http://dx.doi.org/10.1016/j.isci.2021.102815 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ito, Daiyu
Wakayama, Sayaka
Emura, Rina
Ooga, Masatoshi
Wakayama, Teruhiko
Mailing viable mouse freeze-dried spermatozoa on postcards
title Mailing viable mouse freeze-dried spermatozoa on postcards
title_full Mailing viable mouse freeze-dried spermatozoa on postcards
title_fullStr Mailing viable mouse freeze-dried spermatozoa on postcards
title_full_unstemmed Mailing viable mouse freeze-dried spermatozoa on postcards
title_short Mailing viable mouse freeze-dried spermatozoa on postcards
title_sort mailing viable mouse freeze-dried spermatozoa on postcards
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390851/
https://www.ncbi.nlm.nih.gov/pubmed/34471856
http://dx.doi.org/10.1016/j.isci.2021.102815
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