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Gingival-Derived Mesenchymal Stem Cells Protect Against Sepsis and Its Complications

OBJECTIVE: In the present study, we separated and characterized mouse gingival-derived mesenchymal stem cells (GMSCs) and investigated whether GMSCs can improve lipopolysaccharide (LPS)-induced sepsis and its complications. METHODS: Ninety-six ICR mice were randomly divided into the following groups...

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Autores principales: Wang, Xishuai, Song, Hanan, Zhao, Shiyu, Guan, Weijun, Gao, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390887/
https://www.ncbi.nlm.nih.gov/pubmed/34456576
http://dx.doi.org/10.2147/IDR.S318304
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author Wang, Xishuai
Song, Hanan
Zhao, Shiyu
Guan, Weijun
Gao, Yang
author_facet Wang, Xishuai
Song, Hanan
Zhao, Shiyu
Guan, Weijun
Gao, Yang
author_sort Wang, Xishuai
collection PubMed
description OBJECTIVE: In the present study, we separated and characterized mouse gingival-derived mesenchymal stem cells (GMSCs) and investigated whether GMSCs can improve lipopolysaccharide (LPS)-induced sepsis and its complications. METHODS: Ninety-six ICR mice were randomly divided into the following groups: the control (Sham), LPS, and LPS + MSC groups. Mice received 5 mg/kg LPS intraperitoneally to induce sepsis. Histopathological micrographs illustrated organ injury. We detected systemic inflammation, blood glucose levels, and serum levels of high-mobility group box 1 (HMGB1) and lactate. In addition, pulmonary inflammation, lung permeability, and oxidative stress-related indicators in lung tissue were measured. RESULTS: We successfully separated a novel population of MSCs from mouse gingiva. These cells had MSC-associated properties, such as a typical fibroblast-like morphology, multiple differentiation potential, and certain phenotypes. Cell-based therapy using GMSCs significantly improved the survival rate, systemic inflammation, hypoglycemia, multiple organ dysfunction syndrome (MODS), and aortic injury during sepsis. GMSCs administration reduced pulmonary inflammation, lung permeability, and oxidative stress injury. GMSCs administration reduced neutrophil infiltration partly because GMSCs inhibited neutrophil chemoattractants tumor necrosis factor (TNF-α), C-X-C motif chemokine ligand (CXCL-1), and Interleukin (IL-8). GMSCs impaired LPS-induced HMGB1 and lactate release during sepsis. CONCLUSION: GMSCs administration is a novel therapeutic strategy targeting aerobic glycolysis for the treatment of sepsis because GMSCs impair LPS-induced HMGB1 and lactate release. GMSCs alleviate lung injury partly because GMSCs exert immune effects, inhibit neutrophilic inflammation, and reduce oxidative stress injury.
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spelling pubmed-83908872021-08-27 Gingival-Derived Mesenchymal Stem Cells Protect Against Sepsis and Its Complications Wang, Xishuai Song, Hanan Zhao, Shiyu Guan, Weijun Gao, Yang Infect Drug Resist Original Research OBJECTIVE: In the present study, we separated and characterized mouse gingival-derived mesenchymal stem cells (GMSCs) and investigated whether GMSCs can improve lipopolysaccharide (LPS)-induced sepsis and its complications. METHODS: Ninety-six ICR mice were randomly divided into the following groups: the control (Sham), LPS, and LPS + MSC groups. Mice received 5 mg/kg LPS intraperitoneally to induce sepsis. Histopathological micrographs illustrated organ injury. We detected systemic inflammation, blood glucose levels, and serum levels of high-mobility group box 1 (HMGB1) and lactate. In addition, pulmonary inflammation, lung permeability, and oxidative stress-related indicators in lung tissue were measured. RESULTS: We successfully separated a novel population of MSCs from mouse gingiva. These cells had MSC-associated properties, such as a typical fibroblast-like morphology, multiple differentiation potential, and certain phenotypes. Cell-based therapy using GMSCs significantly improved the survival rate, systemic inflammation, hypoglycemia, multiple organ dysfunction syndrome (MODS), and aortic injury during sepsis. GMSCs administration reduced pulmonary inflammation, lung permeability, and oxidative stress injury. GMSCs administration reduced neutrophil infiltration partly because GMSCs inhibited neutrophil chemoattractants tumor necrosis factor (TNF-α), C-X-C motif chemokine ligand (CXCL-1), and Interleukin (IL-8). GMSCs impaired LPS-induced HMGB1 and lactate release during sepsis. CONCLUSION: GMSCs administration is a novel therapeutic strategy targeting aerobic glycolysis for the treatment of sepsis because GMSCs impair LPS-induced HMGB1 and lactate release. GMSCs alleviate lung injury partly because GMSCs exert immune effects, inhibit neutrophilic inflammation, and reduce oxidative stress injury. Dove 2021-08-22 /pmc/articles/PMC8390887/ /pubmed/34456576 http://dx.doi.org/10.2147/IDR.S318304 Text en © 2021 Wang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wang, Xishuai
Song, Hanan
Zhao, Shiyu
Guan, Weijun
Gao, Yang
Gingival-Derived Mesenchymal Stem Cells Protect Against Sepsis and Its Complications
title Gingival-Derived Mesenchymal Stem Cells Protect Against Sepsis and Its Complications
title_full Gingival-Derived Mesenchymal Stem Cells Protect Against Sepsis and Its Complications
title_fullStr Gingival-Derived Mesenchymal Stem Cells Protect Against Sepsis and Its Complications
title_full_unstemmed Gingival-Derived Mesenchymal Stem Cells Protect Against Sepsis and Its Complications
title_short Gingival-Derived Mesenchymal Stem Cells Protect Against Sepsis and Its Complications
title_sort gingival-derived mesenchymal stem cells protect against sepsis and its complications
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390887/
https://www.ncbi.nlm.nih.gov/pubmed/34456576
http://dx.doi.org/10.2147/IDR.S318304
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